Whole-genome sequencing of an NDM-1- and OXA-58-producing Acinetobacter towneri isolate from hospital sewage in Sichuan Province, China

Jiang, Nan; Zhang, Xinzhuo; Zhou, Yingshun; Zhang, Zhikun; Zheng, Xiaoli

doi:10.1016/j.jgar.2018.11.015

Cited by 14 publications

(14 citation statements)

References 5 publications

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“…One Citrobacter braakii strain was identified co-harboring bla NDM-4 and bla OXA-48 from hospital sewage in northern India [15]. An Acinetobacter towneri strain was identified co-harboring bla NDM-1 and bla OXA-58 from hospital sewage from one of our sampling sites (hospital A) [23]. In our work, the coexistence of bla NDM-1 and bla OXA-58 genes was identified in six Acinetobacter isolates, of which five were from hospital C and one from hospital B. Additionally, one Acinetobacter strain was found to co-harbor the bla NDM-5 and bla OXA-58 genes.…”

Section: Discussionmentioning

confidence: 90%

“…The first report concerning carbapenemases-producing bacteria (CPB) in hospital sewage in China in 2012 identified bla KPC-2 -carrying Citrobacter freundii and Enterobacter cloacae in the influx of the wastewater treatment plants (WWTPs) [19]. After that, studies have detected a rich variety of resistant bacteria that produce carbapenemases, including NDM-1, KPC-2, and OXA-58 in hospital sewage in China [20][21][22][23]. Rivers are natural receptacles for large amounts of microbial contaminants from domestic sewage, livestock wastewater, and hospital effluents.…”

Section: Introductionmentioning

confidence: 99%

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The Prevalence and Characterization of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Bacteria from Hospital Sewage, Treated Effluents and Receiving Rivers

Zhang

Luo

et al. 2020

IJERPH

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Hospital sewage plays a key role in the dissemination of antibiotic-resistant genes (ARGs) by serving as an environmental antimicrobial resistance reservoir. In this study, we aimed to characterize the cephalosporin- and carbapenem-resistant isolates from hospital sewage and receiving rivers. The results showed that ESBL (blaCTX-M) and carbapenemase genes (blaNDM and blaKPC) were widely detected in a number of different bacterial species. These resistance genes were mainly harbored in Enterobacteriaceae, followed by Acinetobacter and Aeromonas isolates. More attention should be given to these bacteria as important vectors of ARGs in the environment. Furthermore, we showed that the multidrug resistance phenotype was highly prevalent, which was found in 85.5% Enterobacteriaceae and 75% Acinetobacter strains. Notably, the presence of carbapenemase genes in isolates from treated effluents and receiving rivers indicates that the discharges of wastewater treatment plants could be an important source for high-risk resistance genes propagation to the environment. In conclusion, this study shows a high prevalence of ESBL- and carbapenemase-producing bacteria in hospital sewage and receiving rivers in China. These findings have serious implications for human health, and also suggest the need for more efforts to control the dissemination of resistant bacteria from hospital sewage into the environment.

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Section: Discussionmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

The Prevalence and Characterization of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Bacteria from Hospital Sewage, Treated Effluents and Receiving Rivers

Zhang

Luo

et al. 2020

IJERPH

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show abstract

“…This indicates the need for further investigations in the context of genomic epidemiological characteristics of OXA-58 as well as its clinical and microbiological significance on carbapenem resistance in Acinetobacter species. A third observation is the detection of OXA-58 in Acinetobacter towneri and in 9 environmental genera of bacteria from hospital sewage [154] and coastal water [155], respectively.…”

Section: Ambler Class D Carbapenemases (Oxacillinases)mentioning

confidence: 96%

“…Xin et al reported pollution of estuarine water with bacterial genera including Rheinheimera, Stenotrophomonas, Shewanella, Raoultella, Vibrio, Pseudoalteromonas, Algoriphagus, Bowmanella, and Thalassospira, which all harbor bla OXA-58 [155] conferring resistance to carbapenems and penicillin and an ability to hydrolyze cefpirome and cephalothin but not ceftazidime, cefotaxime, or cefepime. Citrobacter freundii OXA-204 [171] Hospital wastewater E. coli KPC-2 [64] Raoltella ornitholitica KPC-2 [270] Acinetobacter towneri OXA-58 [154] Coastal water A. towneri OXA-58 [155] Municipal water Acinetobacter lwoffii OXA-235 and OXA-278 [163] River water Klebsiella pneumoniae KPC-2 [216] Estuarine water…”

Section: Other Gram-negative Organismsmentioning

confidence: 99%

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

2020

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Carbapenemases are β-lactamases belonging to different Ambler classes (A, B, D) and can be encoded by both chromosomal and plasmid-mediated genes. These enzymes represent the most potent β-lactamases, which hydrolyze a broad variety of β-lactams, including carbapenems, cephalosporins, penicillin, and aztreonam. The major issues associated with carbapenemase production are clinical due to compromising the activity of the last resort antibiotics used for treating serious infections, and epidemiological due to their dissemination into various bacteria across almost all geographic regions. Carbapenemase-producing Enterobacteriaceae have received more attention upon their first report in the early 1990s. Currently, there is increased awareness of the impact of nonfermenting bacteria, such as Acinetobacter baumannii and Pseudomonas aeruginosa, as well as other Gram-negative bacteria that are carbapenemase-producers. Outside the scope of clinical importance, carbapenemases are also detected in bacteria from environmental and zoonotic niches, which raises greater concerns over their prevalence, and the need for public health measures to control consequences of their propagation. The aims of the current review are to define and categorize the different families of carbapenemases, and to overview the main lines of their spread across different bacterial groups.

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“…In various bacteria, over 400 OXA-type enzymes are already known, of which many are carbapenemases [133]. A. baumannii isolates harboring plasmid-encoded OXA-23, OXA-24/40, OXA-58, OXA-143, and OXA-235 families have appeared from the 1980s onwards; chromosomal loci encoding some of these enzymes were also identified [20,[160][161][162][163].…”

Section: Ambler Class D Enzymesmentioning

confidence: 99%

Insights into Acinetobacter baumannii: A Review of Microbiological, Virulence, and Resistance Traits in a Threatening Nosocomial Pathogen

2020

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Being a multidrug-resistant and an invasive pathogen, Acinetobacter baumannii is one of the major causes of nosocomial infections in the current healthcare system. It has been recognized as an agent of pneumonia, septicemia, meningitis, urinary tract and wound infections, and is associated with high mortality. Pathogenesis in A. baumannii infections is an outcome of multiple virulence factors, including porins, capsules, and cell wall lipopolysaccharide, enzymes, biofilm production, motility, and iron-acquisition systems, among others. Such virulence factors help the organism to resist stressful environmental conditions and enable development of severe infections. Parallel to increased prevalence of infections caused by A. baumannii, challenging and diverse resistance mechanisms in this pathogen are well recognized, with major classes of antibiotics becoming minimally effective. Through a wide array of antibiotic-hydrolyzing enzymes, efflux pump changes, impermeability, and antibiotic target mutations, A. baumannii models a unique ability to maintain a multidrug-resistant phenotype, further complicating treatment. Understanding mechanisms behind diseases, virulence, and resistance acquisition are central to infectious disease knowledge about A. baumannii. The aims of this review are to highlight infections and disease-producing factors in A. baumannii and to touch base on mechanisms of resistance to various antibiotic classes.

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Whole-genome sequencing of an NDM-1- and OXA-58-producing Acinetobacter towneri isolate from hospital sewage in Sichuan Province, China

Cited by 14 publications

References 5 publications

The Prevalence and Characterization of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Bacteria from Hospital Sewage, Treated Effluents and Receiving Rivers

The Prevalence and Characterization of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing Bacteria from Hospital Sewage, Treated Effluents and Receiving Rivers

The Current Burden of Carbapenemases: Review of Significant Properties and Dissemination among Gram-Negative Bacteria

Insights into Acinetobacter baumannii: A Review of Microbiological, Virulence, and Resistance Traits in a Threatening Nosocomial Pathogen

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