Whole-genome sequencing of bacterial sexually transmitted infections

Seth-Smith, Helena M. B.; Thomson, Nicholas R.

doi:10.1097/qco.0b013e32835c2159

Cited by 17 publications

(15 citation statements)

References 79 publications

(69 reference statements)

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“…Comparative genomic studies on chlamydia have demonstrated that it undergoes extensive recombination. Genome projects can also help us understand the phenomenon of antibiotic resistance in STD agents, particularly in the cases of C. trachomatis, where very little was known about the genetics of these organisms because of a lack of molecular tools and the inability to grow them outside cell cultures or host organisms [14].…”

Section: Discussionmentioning

confidence: 99%

“…Increased usage of nucleic acid amplification tests (NAATs) in the diagnosis of STD leads to the elimination of culture-based techniques, depleting the number of live strains available for resistance testing. A lack of routine test-of-cure makes it impossible to distinguish treatment failure from reinfection [14]. The cost of health care for patients with resistant infections is higher due to longer duration of illness, additional tests and use of more expensive drugs.…”

Section: Introductionmentioning

confidence: 99%

“…Following the endocytosis of the small infectious bodies into the host cell and their inclusion into specialized vesicles, C. trachomatis strains differentiate into metabolically active reticular bodies and replicate until the inclusion fills the host cell. At this stage, the reticular bodies differentiate into elementary bodies and are being released from the cell to continue the infection [14].…”

Section: Introductionmentioning

confidence: 99%

“…However, N. gonorrhoeae infections tend to be more acute, with symptoms including purulent discharge and acute local inflammation. Rashes and septic arthritis may occur if the bacterium disseminates through the host [14].…”

Section: Introductionmentioning

confidence: 99%

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Determining the Antibiotic Resistance of Bacterial Pathogens in Sexually Transmitted Diseases

Laura¹,

Vladi²,

Vasile³

2017

Antibacterial Agents

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Sexually transmitted diseases (STD) are among the most common infections worldwide. These bacterial infections have spread predominantly in the developing/underdeveloped countries, the most common being syphilis, gonorrhea and those induced by Chlamydia trachomatis, Ureaplasma urealyticum or Mycoplasma spp. Due to extensive usage of antibiotics in the recent past, these bacteria developed resistance to those commonly used for treatment, such resistant strains becoming a public health problem in a number of countries. It is well documented that bacterial STD agents are difficult to detect using standard culture media because these methods require special conditions and adequate nutrients. Antimicrobial susceptibility testing is, therefore, difficult to obtain in such cases. In recent years, genetic tests have been frequently employed in STD diagnosis. The study of genes that induce resistance to antibiotics using DNA isolated from these bacteria may prove to be a viable alternative. Genetic methods enable the DNA extraction from different biological samples, and both the presence of the bacteria and their resistance to one or more antibiotics can be determined from a single DNA sample. By studying the genes that induce antibiotic resistance and the plasmids that transfer such genes, the mechanism that leads to antibiotic resistance can be elucidated.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Determining the Antibiotic Resistance of Bacterial Pathogens in Sexually Transmitted Diseases

Laura¹,

Vladi²,

Vasile³

2017

Antibacterial Agents

View full text Add to dashboard Cite

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“…This method enriches for the targeted bacteria in low burden samples and includes an amplification of the target DNA in sufficient quantities suitable for whole-genome sequencing. The method has the advantages of being nonculture based, highly portable and very well suited for de novo sequence assembly The IMS-MDA has been successfully implemented with respect to the obligate intracellular pathogen Chlamydia trachomatis Seth-Smith & Thomson, 2013).…”

Section: Future Directionsmentioning

confidence: 99%

Whole-Genome Sequencing for Rapid and Accurate Identification of Bacterial Transmission Pathways

Harris

Okoro

2014

Methods in Microbiology

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Diagnosis of Chlamydia trachomatis genital infections in the era of genomic medicine

Shetty

Kouskouti

Schoen³

et al. 2021

Braz J Microbiol

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Purpose Chlamydial genital infections constitute significant sexually transmitted infections worldwide. The often asymptomatic status of C. trachomatis (CT) infections leads to an increased burden on human reproductive health, especially in middle- and low-income settings. Early detection and management of these infections could play a decisive role in controlling this public health burden. The objective of this review is to provide an insight into the evolution of diagnostic methods for CT infections through the development of new molecular technologies, emphasizing on -omics’ technologies and their significance as diagnostic tools both for effective patient management and control of disease transmission. Methods Narrative review of the diagnostic methodologies of CT infections and the impact of the introduction of -omics’ technologies on their diagnosis by review of the literature. Results Various methodologies are discussed with respect to working principles, required specifications, advantages, and disadvantages. Implementing the most accurate methods in diagnosis is highlighted as the cornerstone in managing CT infections. Conclusion Diagnostics based on -omics’ technologies are considered to be the most pertinent modalities in CT testing when compared to other available methods. There is a need to modify these effective and accurate diagnostic tools in order to render them more available and feasible in all settings, especially aiming on turning them to rapid point-of-care tests for effective patient management and disease control.

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Whole-genome sequencing of bacterial sexually transmitted infections

Cited by 17 publications

References 79 publications

Determining the Antibiotic Resistance of Bacterial Pathogens in Sexually Transmitted Diseases

Determining the Antibiotic Resistance of Bacterial Pathogens in Sexually Transmitted Diseases

Whole-Genome Sequencing for Rapid and Accurate Identification of Bacterial Transmission Pathways

Diagnosis of Chlamydia trachomatis genital infections in the era of genomic medicine

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