Whole genome sequencing of field isolates reveals extensive genetic diversity in Plasmodium vivax from Colombia

Winter, David J.; Pacheco, M. Andreína; Vallejo, Andrés F.; Schwartz, Rachel S.; Arévalo‐Herrera, Myriam; Cartwright, R.A.; Escalante, Ananías A.

doi:10.1101/025338

Cited by 3 publications

(1 citation statement)

References 71 publications

(126 reference statements)

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“…However, the cost is a significant impediment to implementing WGS and it remains impractical in many situations due to technical constraints connected with large-scale data storage and processing. These studies cannot usually report diversity statistics due to multiple infections in their samples ( 82 ).…”

Section: Genomic Approaches and Tools For Measuring Malaria Transmiss...mentioning

confidence: 99%

Genomic approaches for monitoring transmission dynamics of malaria: A case for malaria molecular surveillance in Sub–Saharan Africa

Mensah

Akyea-Bobi²,

Ghansah³

2022

Front. Epidemiol.

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Transmission dynamics is an important indicator for malaria control and elimination. As we move closer to eliminating malaria in Sub-Saharan Africa (sSA), transmission indices with higher resolution (genomic approaches) will complement our current measurements of transmission. Most of the present programmatic knowledge of malaria transmission patterns are derived from assessments of epidemiologic and clinical data, such as case counts, parasitological estimates of parasite prevalence, and Entomological Inoculation Rates (EIR). However, to eliminate malaria from endemic areas, we need to track changes in the parasite population and how they will impact transmission. This is made possible through the evolving field of genomics and genetics, as well as the development of tools for more in-depth studies on the diversity of parasites and the complexity of infections, among other topics. If malaria elimination is to be achieved globally, country-specific elimination activities should be supported by parasite genomic data from regularly collected blood samples for diagnosis, surveillance and possibly from other programmatic interventions. This presents a unique opportunity to track the spread of malaria parasites and shed additional light on intervention efficacy. In this review, various genetic techniques are highlighted along with their significance for an enhanced understanding of transmission patterns in distinct topological settings throughout Sub-Saharan Africa. The importance of these methods and their limitations in malaria surveillance to guide control and elimination strategies, are explored.

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Section: Genomic Approaches and Tools For Measuring Malaria Transmiss...mentioning

confidence: 99%

Genomic approaches for monitoring transmission dynamics of malaria: A case for malaria molecular surveillance in Sub–Saharan Africa

Mensah

Akyea-Bobi²,

Ghansah³

2022

Front. Epidemiol.

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Whole Genome Sequencing Contributions and Challenges in Disease Reduction Focused on Malaria

Akoniyon

Maharaj

Roux

et al. 2022

Biology

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Malaria elimination remains an important goal that requires the adoption of sophisticated science and management strategies in the era of the COVID-19 pandemic. The advent of next generation sequencing (NGS) is making whole genome sequencing (WGS) a standard today in the field of life sciences, as PCR genotyping and targeted sequencing provide insufficient information compared to the whole genome. Thus, adapting WGS approaches to malaria parasites is pertinent to studying the epidemiology of the disease, as different regions are at different phases in their malaria elimination agenda. Therefore, this review highlights the applications of WGS in disease management, challenges of WGS in controlling malaria parasites, and in furtherance, provides the roles of WGS in pursuit of malaria reduction and elimination. WGS has invaluable impacts in malaria research and has helped countries to reach elimination phase rapidly by providing required information needed to thwart transmission, pathology, and drug resistance. However, to eliminate malaria in sub-Saharan Africa (SSA), with high malaria transmission, we recommend that WGS machines should be readily available and affordable in the region.

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Malaria in the ‘Omics Era’

Pegoraro

Weedall

2021

Genes

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Genomics has revolutionised the study of the biology of parasitic diseases. The first Eukaryotic parasite to have its genome sequenced was the malaria parasite Plasmodium falciparum. Since then, Plasmodium genomics has continued to lead the way in the study of the genome biology of parasites, both in breadth—the number of Plasmodium species’ genomes sequenced—and in depth—massive-scale genome re-sequencing of several key species. Here, we review some of the insights into the biology, evolution and population genetics of Plasmodium gained from genome sequencing, and look at potential new avenues in the future genome-scale study of its biology.

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Whole genome sequencing of field isolates reveals extensive genetic diversity in Plasmodium vivax from Colombia

Cited by 3 publications

References 71 publications

Genomic approaches for monitoring transmission dynamics of malaria: A case for malaria molecular surveillance in Sub–Saharan Africa

Genomic approaches for monitoring transmission dynamics of malaria: A case for malaria molecular surveillance in Sub–Saharan Africa

Whole Genome Sequencing Contributions and Challenges in Disease Reduction Focused on Malaria

Malaria in the ‘Omics Era’

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