2018
DOI: 10.1128/genomea.01424-17
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Whole-Genome Sequencing of Six Borrelia miyamotoi Clinical Strains Isolated in Russia

Abstract: Here, we report the whole-genome sequence of six clinical Borrelia miyamotoi isolates from the Russian Federation. Using two independent next-generation sequencing platforms, we determined the complete sequence of the chromosome and several plasmids. All strains have an Asian genotype with 99.8% chromosome nucleotide similarity with B. miyamotoi strain FR64b.

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Cited by 20 publications
(18 citation statements)
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“…1). The first three non-chromosomal fragments with sizes ranging from 72 kb to 64 kb were similar among all Russian B. miyamotoi isolates [44] (data not shown). The remaining bands indicated the presence of additional six plasmids with sizes ranging from approx.…”
Section: Pfge Analysis Of B Miyamotoi Izh-4 Strainmentioning
confidence: 73%
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“…1). The first three non-chromosomal fragments with sizes ranging from 72 kb to 64 kb were similar among all Russian B. miyamotoi isolates [44] (data not shown). The remaining bands indicated the presence of additional six plasmids with sizes ranging from approx.…”
Section: Pfge Analysis Of B Miyamotoi Izh-4 Strainmentioning
confidence: 73%
“…For some bacteria the assembly of completed genomes had been reported using Nanopore sequencing as sole method [57] or combining long-read with Illumina short-read technology [43,58,59]. Initial assemblies combining Illumina and Nanopore reads of B. miyamotoi genomes from Russia gave unsatisfactory results, as not all genomic elements (plasmids) were properly assembled [44]. Similar issues were encountered before with B. miyamotoi genomes, i.e.…”
Section: Discussionmentioning
confidence: 92%
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“…miyamotoi populations, comparative genomics should be employed, but this requires high quality reference genomes [56]. Although several B. miyamotoi draft genomes from North America, Russia and Japan exist [11,14,42,44], a completed genome has not been finished for the Asian genotype of B. miyamotoi. Therefore, to provide a basis for comparative genomics studies, here we assembled a reference genome for the Russian B. miyamotoi isolate Izh-4 using currently available long-read and short-read technologies.…”
Section: Discussionmentioning
confidence: 99%