Whole Agrocybe cylindracea Prevented Obesity Linking with Modification of Gut Microbiota and Associated Fecal Metabolites in High‐Fat Diet‐Fed Mice

Abstract

Scope: Whole-food-based strategies to prevent metabolic diseases are growing interests. Agrocybe cylindracea (AC) is a major edible mushroom with high values of nutrition, but little is known about its health benefits as a portion of whole food. Methods and Results: Diet-induced obese, C57BL/6J mice are fed an high-fat diet (HFD) with or without AC (3% or 5%, w/w in the diet) for 9 weeks. The results show that dietary AC reduced body weight, adipose accumulation, impairment of glucose tolerance, lipid levels, and liver injury in HFD-fed mice. Moreover, AC not only prevents HFD-induced gut disorder, as indicates by the enriched probiotic Bifidobacterium and reduced endotoxin-bearingProteobacteria, but also improve the lipopolysaccharide (LPS) level and gut tissue structure. Fecal metabolites such as harmine and harmanine are also remarkably altered by AC. Spearman's correlation analysis reveals that the AC-altered microbes and metabolites are strongly correlated with obesity-related indexes. Conclusion: These findings suggest that dietary AC prevents HFD-induced obesity and its complications in association with modulating gut microbiota and associated fecal metabolites.

Modulatory Effects of Lactarius hatsudake on Obesity and Gut Microbiota in High-Fat Diet-Fed C57BL/6 Mice

“…A growing body of research has demonstrated that edible mushrooms and their extracts can ameliorate obesity [ 19 , 20 ]. Consistent with findings in prior studies, adding whole Agrocybe cylindracea [ 5 , 19 ] and Pleurotus ostreatus [ 21 ] to the diet of mice fed a HFD resulted in decreased weight gain and liver weight in a manner that depended on the dosage. Conversely, a study found that consuming a combination of Japanese mushrooms at levels of 0.5% and 3% in a HFD did not lead to a significant reduction in the body weight of mice, similar to LH, but did result in a decrease in the weight of white adipose tissue and perinephric adipose tissue [ 22 ].…”

“…There are now almost 2 billion overweight adults in the world, and this trend continues to worsen, making obesity a global epidemic [ 3 ]. Obesity and its aggravation of a number of major diseases have become an issue of public health threat, which negatively impacts the quality and longevity of human life and the cost of healthcare [ 4 , 5 ]. Thus, it is a major challenge for us to prevent or ameliorate obesity.…”

“…For instance, switching animal diets from low-fat to high-fat leads to a decrease in Bacteroides and an increase in Firmicutes in the gut microbiota, which is associated with higher energy absorption, fat accumulation, and ultimately gut inflammation and permeability [ 11 ]. Research has demonstrated that certain food items and their active ingredients (like antibiotics, prebiotics, and probiotics) can be targeted to modify the makeup and arrangement of gut bacteria, potentially impeding the onset of obesity [ 2 , 4 , 5 ].…”

Selenium-containing polysaccharide from Spirulina platensis alleviates Cd-induced toxicity in mice by inhibiting liver inflammation mediated by gut microbiota

“…β-Diversity analysis is used to evaluate the similarity of the community structure between different groups ( 29 ). Principal co-ordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS) analysis were used to represent community changes in different samples.…”

Therapeutic applications of gut microbes in cardiometabolic diseases: current state and perspectives

“…An increasing body of evidence suggests that gut flora is associated with the development of obesity and associated metabolic diseases (Torres-Fuentes et al 2017 ; Zhu et al 2022 ). Gut flora is closely associated to host food digestion, nutrient absorption, energy metabolism, and central appetite, which are all associated with obesity (Wijdeveld et al 2020 ).…”