Whole mount preparations of rat and mouse mammary glands

Samoǐlov, V. I.; Chebyshev, A. P.

doi:10.1007/bf00792969

Cited by 3 publications

(2 citation statements)

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“…Various dilutions and staining times were evaluated for cresyl violet, neutral red [15] and thionin, and as described by Samoilov and Chebyshev [21], simultaneous postfixation and thionin staining were attempted using various dilutions of thionin and formaldehyde.…”

Section: Methodsmentioning

confidence: 99%

Subgross breast pathology in the twenty-first century

Smith

Going

2012

Virchows Arch

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Subgross studies on breast morphology in cleared thick sections are informative, but lengthy protocols could clash with diagnostic timescales and interfere with immunohistochemistry (IHC) or molecular analysis. We sought to speed up staining and tissue clearing to improve compatibility with diagnostic needs without detriment to histology or other assays. Thick sections (2-3 mm) of normal human breast tissue and whole mouse mammary glands were stained with alum-carmine, aceto-carmine, Harris's haematoxylin, cresyl violet, neutral red, thionin, 4',6-diamidino-2-phenylindole (DAPI), ethidium bromide (EB), or propidium iodide (PI) and cleared in xylene (refractive index ν = 1.50), benzyl alcohol/benzyl benzoate (BABB; ν = 1.59), xylene-BABB (X-BABB), thiodiglycol (2,2'-thiodiethanol, ν = 1.52) or anethole (ν = 1.56). Tissue was then paraffin embedded for IHC for ER, PR, E-cadherin, CD31 or cytokeratin 7. Haematoxylin and alum-carmine are excellent non-fluorescent subgross stains giving strong nuclear staining and minimal background. DAPI and EB permeate thick sections poorly but PI penetrates well, with a high signal-to-noise ratio after clearing in BABB or X-BABB. Other clearing agents were less effective, including thiodiglycol. Anethole's unpleasant odour precluded further evaluation. All evaluated clearing agents preserved comparable immunoreactivity for all markers. PI is a promising stain for subgross breast studies, compatible with BABB clearing. The hope that the water-miscible thioalcohol, thiodiglycol, might accelerate tissue clearing was not realised. There is scope for further streamlining to make subgross techniques more acceptable in combined research/diagnostic settings.

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Section: Methodsmentioning

confidence: 99%

Subgross breast pathology in the twenty-first century

Smith

Going

2012

Virchows Arch

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“…Whole mounts allow for three‐dimensional assessment of the entire gland and fat pad and can offer quantitative assessment of the mammary gland, including descriptions of longitudinal growth, lateral growth, TEB counts, and more recently, quantification of the mammary gland branching characteristics using Scholl analysis (Stanko, Easterling, & Fenton, ). Mammary gland whole mounts have been utilized to study changes in the structure of these developing glands since at least the 1940s (Samoilov and Cherbyshev, ). These methods are particularly useful as a disruption in normal mammary gland morphology, which may result in functional change in the gland or an increased susceptibility to tumor formation later in life (Fenton, ; Rudel, Fenton, Ackerman, Euling, & Makris, ).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the Estrous Cycle, Reproductive Tract, and Mammary Gland in Female Mice

Vidal

Filgo

2017

CP Mouse Biology

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Evaluation of the female reproductive system is an important part of basic reproductive biology research, toxicology testing, and mutant mouse phenotype assessment. The female reproductive system is dynamic and the onset of puberty and the normal changes observed during estrous cyclicity can create challenges for an investigator. Experimental work in the female mouse requires an understanding of the potential impact of the estrous cycle and tracking normal changes throughout the cycle allows for control of this key variable. The estrous cycle can be evaluated using vaginal cytology, which provides the researcher a daily assessment of the entire reproductive endocrine axis and allows for samples to be collected at precise times within the cycle. These protocols describe the basic approach to evaluating the onset of cyclicity, tracking the normal cycle, and collection and microscopic evaluation of the reproductive system and mammary gland in the mouse. © 2017 by John Wiley & Sons, Inc.

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Pathology of the Male and Female Reproductive System and Mammary Gland

Vidal

2019

Toxicologic Pathology for Non-Pathologists

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Whole mount preparations of rat and mouse mammary glands

Cited by 3 publications

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Subgross breast pathology in the twenty-first century

Subgross breast pathology in the twenty-first century

Evaluation of the Estrous Cycle, Reproductive Tract, and Mammary Gland in Female Mice

Pathology of the Male and Female Reproductive System and Mammary Gland

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