Whole Root Transcriptomic Analysis Suggests a Role for Auxin Pathways in Resistance to<i>Ralstonia solanacearum</i>in Tomato

French, Elizabeth; Kim, Bong-Suk; Rivera-Zuluaga, Katherine; Iyer‐Pascuzzi, Anjali S.

doi:10.1094/mpmi-08-17-0209-r

Cited by 64 publications

(69 citation statements)

References 83 publications

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“…Because many of the genes that were activated or inactivated also suffered changes in protein abundance, we checked their expression levels on R. solanacearum natural infection. Available gene expression data (55) showed that gene expression correlated with protein abundance in our study (supplemental Fig. S7A).…”

Section: The Tomato Cultivar Hawaii 7996 Survives Leaf Necrosis Causesupporting

confidence: 58%

Protease Activities Triggered by Ralstonia solanacearum Infection in Susceptible and Tolerant Tomato Lines

Planas-Marquès

Bernardo-Faura

Paulus

et al. 2018

Molecular & Cellular Proteomics

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Activity-based protein profiling (ABPP) is a powerful proteomic technique to display protein activities in a proteome. It is based on the use of small molecular probes that react with the active site of proteins in an activity-dependent manner. We used ABPP to dissect the protein activity changes that occur in the intercellular spaces of tolerant (Hawaii 7996) and susceptible (Marmande) tomato plants in response to R. solanacearum, the causing agent of bacterial wilt, one of the most destructive bacterial diseases in plants. The intercellular space -or apoplast- is the first battlefield where the plant faces R. solanacearum. Here, we explore the possibility that the limited R. solanacearum colonization reported in the apoplast of tolerant tomato is partly determined by its active proteome. Our work reveals specific activation of papain-like cysteine proteases (PLCPs) and serine hydrolases (SHs) in the leaf apoplast of the tolerant tomato Hawaii 7996 on R. solanacearum infection. The P69 family members P69C and P69F, and an unannotated lipase (Solyc02g077110.2.1), were found to be post-translationally activated. In addition, protein network analysis showed that deeper changes in network topology take place in the susceptible tomato variety, suggesting that the tolerant cultivar might be more prepared to face R. solanacearum in its basal state. Altogether this work identifies significant changes in the activity of 4 PLCPs and 27 SHs in the tomato leaf apoplast in response to R. solanacearum, most of which are yet to be characterized. Our findings denote the importance of novel proteomic approaches such as ABPP to provide new insights on old and elusive questions regarding the molecular basis of resistance to R. solanacearum.

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Section: The Tomato Cultivar Hawaii 7996 Survives Leaf Necrosis Causesupporting

confidence: 58%

Protease Activities Triggered by Ralstonia solanacearum Infection in Susceptible and Tolerant Tomato Lines

Planas-Marquès

Bernardo-Faura

Paulus

et al. 2018

Molecular & Cellular Proteomics

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“…The gene Araip.ZD3PG was predicted to code for auxin response factor, which may involve in the regulation of auxin pathway. Recent reports showed that the repression of auxin pathway improved the resistance to bacterial pathogen (French et al ., ; Navarro, ). The gene Araip.CMC8E might code for calcium‐dependent protein kinase, which could perceive changes in intracellular calcium concentrations to transmit defence signal in response to PAMPs and pathogen effectors (Liu et al ., ; Wang et al ., 2018a).…”

Section: Discussionmentioning

confidence: 99%

Next‐generation sequencing identified genomic region and diagnostic markers for resistance to bacterial wilt on chromosome B02 in peanut (Arachis hypogaea L.)

Luo

Pandey

Khan

et al. 2019

Plant Biotechnology Journal

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Summary Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting over 350 plant species. A few peanut cultivars were found to possess stable and durable bacterial wilt resistance (BWR). Genomics‐assisted breeding can accelerate the process of developing resistant cultivars by using diagnostic markers. Here, we deployed sequencing‐based trait mapping approach, QTL‐seq, to discover genomic regions, candidate genes and diagnostic markers for BWR in a recombination inbred line population (195 progenies) of peanut. The QTL‐seq analysis identified one candidate genomic region on chromosome B02 significantly associated with BWR. Mapping of newly developed single nucleotide polymorphism (SNP) markers narrowed down the region to 2.07 Mb and confirmed its major effects and stable expressions across three environments. This candidate genomic region had 49 nonsynonymous SNPs affecting 19 putative candidate genes including seven putative resistance genes (R‐genes). Two diagnostic markers were successfully validated in diverse breeding lines and cultivars and could be deployed in genomics‐assisted breeding of varieties with enhanced BWR.

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“…Our understanding of the role of auxin in plant-microbe interactions is limited and research efforts have largely included genetic and molecular investigations of pathogenic interactions, transcriptomics or proteomics changes associated with plant-microbe interactions, and a limited number of host genetic and mutant evaluations with beneficial microbes such as under nodulation (Grunewald et al 2009;Zhao 2010;Sukumar et al 2013;Contreras-Cornejo et al 2015;Boivin et al 2016;Poupin et al 2016;French et al 2018;Estenson et al 2018). Auxin action can be regulated at the levels of biosynthesis, transport, and signal transduction (Lavy and Estelle, 2016).…”

Section: Introductionmentioning

confidence: 99%

Host auxin machinery is important in mediating root architecture changes induced byPseudomonas fluorescensGM30 andSerendipita indica

Matthiadis

Sukumar

DeLeon

et al. 2020

Preprint

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Auxin is a key phytohormone that is integral to plant developmental processes including those underlying root initiation, elongation, and branching. Beneficial microbes have been shown to have an impact on root development, potentially mediated through auxin. In this study, we explore the role of host auxin signaling and transport components in mediating the root growth promoting effects of beneficial microbes. Towards this end, we undertook co-culture studies of Arabidopsis thaliana plants with microbes previously reported to promote lateral root proliferation and produce auxin. Two types of beneficial microbes were included in the present study; a plant growth promoting bacterial species of interest, Pseudomonas fluorescens GM30, and a well-studied plant growth promoting fungal species, Serendipita indica (Piriformospora indica). Following co-culture, lateral root production was found partially restored in auxin transport inhibitor-treated plants, suggesting involvement of microbe and/or microbially-produced auxin in altering plant auxin levels. In order to clarify the role of host auxin signaling and transport pathways in mediating interactions with bacterial and fungal species, we employed a suite of auxin genetic mutants as hosts in co-culture screens. Our result show that the transport proteins PIN2, PIN3, and PIN7 and the signaling protein ARF19, are required for mediating root architecture effects by the bacterial and/or fungal species. Mutants corresponding to these proteins did not significantly respond to co-culture treatment and did not show increases in lateral root production and lateral root density. These results implicate the importance of host auxin signaling in both bacterial and fungal induced changes in root architecture and serve as a driver for future research on understanding the role of auxin-dependent and auxin-independent pathways in mediating plant-microbe interactions in economically important crop species.

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Whole Root Transcriptomic Analysis Suggests a Role for Auxin Pathways in Resistance toRalstonia solanacearumin Tomato

Cited by 64 publications

References 83 publications

Protease Activities Triggered by Ralstonia solanacearum Infection in Susceptible and Tolerant Tomato Lines

Protease Activities Triggered by Ralstonia solanacearum Infection in Susceptible and Tolerant Tomato Lines

Next‐generation sequencing identified genomic region and diagnostic markers for resistance to bacterial wilt on chromosome B02 in peanut (Arachis hypogaea L.)

Host auxin machinery is important in mediating root architecture changes induced byPseudomonas fluorescensGM30 andSerendipita indica

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