Whole-transcriptome analysis of endothelial to hematopoietic stem cell transition reveals a requirement for Gpr56 in HSC generation

Abstract

Using highly sensitive RNAseq to examine the whole transcriptome of enriched aortic hematopoietic stem cells and endothelial cells, the authors find G-protein–coupled receptor, Gpr56, is required to generate the first HSCs during endothelial to hematopoietic cell transition.

Identification of novel regulators of developmental hematopoiesis using Endoglin regulatory elements as molecular probes

“…[16][17][18] Endoglin (ENG) is an accessory receptor and modulator of TGF-b superfamily signaling. 19 ENG is expressed on FLK11 mesoderm and is required for normal BL-CFC development, and its expression facilitates the hematopoietic program in these cells.…”

Functional and molecular characterization of mouse Gata2-independent hematopoietic progenitors

“…Analysis of FPKMs for heptad transcription factors previously described as expressed in AGM HSCs and HPCs [1][2][3] showed expression in both the Venus 1 and Venus 2 AGM fractions (data not shown). Also, other Gata factors were expressed in both fractions.…”

“…1 It is upregulated in vivo in Ly6aGFP 1 cells undergoing endothelial-to-hematopoietic cell transition (EHT), a process by which definitive hematopoietic progenitors (HPCs) and hematopoietic stem cells (HSCs) are generated in the embryo. 2,3 As one of the major regulators of HPC and HSC generation, germline deficiency of Gata2 results in embryonic lethality between embryonic day (E)10 and E10.5 and an anemic phenotype, with a decreased number of primitive and definitive HPCs in the yolk sac (YS) and in Gata2 2/2 embryonic stem (ES) cell hematopoietic differentiation cultures. [4][5][6] Chimeric embryo generation with Gata2 2/2 ES cells revealed defective production of all hematopoietic lineages.…”

Interplay between SOX7 and RUNX1 regulates hemogenic endothelial fate in the yolk sac

“…(C,D) Ingenuity pathway analysis of the genes suppressed by SOX7 at 6 h. (E) Ingenuity upstream analysis (IUA) of the genes suppressed by SOX7 at 6 h. Genes suppressed by SOX7 are indicated in green. GPR56 was not picked up in IUA, but has been shown to be regulated by RUNX1 (Solaimani Kartalaei et al, 2015). (F) Overlap between the genes suppressed by SOX7 at 6 h, with genes bound by RUNX1 and TAL1 in HPC-7 (HPC-7 ChIP dataset from Wilson et al, 2010).…”