2017
DOI: 10.1038/labinvest.2017.82
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Whole tumor section quantitative image analysis maximizes between-pathologists' reproducibility for clinical immunohistochemistry-based biomarkers

Abstract: Pathologists have had increasing responsibility for quantitating immunohistochemistry (IHC) biomarkers with the expectation of high between-reader reproducibility due to clinical decision-making especially for patient therapy. Digital imaging-based quantitation of IHC clinical slides offers a potential aid for improvement; however, its clinical adoption is limited potentially due to a conventional field-of-view annotation approach. In this study, we implemented a novel solely morphology-based whole tumor secti… Show more

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Cited by 20 publications
(21 citation statements)
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“…Hematoxylin and eosin (H&E)-stained sections along with the immunostained slides were scanned on VENTANA iSCAN HT scanner (Figure 1). Three independent pathologists manually annotated H&E sections to outline the entire tumor region containing invasive cancer ( i.e., core of the tumor [CT]) using a whole tumor section approach (31). They further demarcated the invasive margin (IM) without knowledge of clinical characteristics or outcome by indicating sections of the tumor outline involved in the invasive process.…”
Section: Methodsmentioning
confidence: 99%
“…Hematoxylin and eosin (H&E)-stained sections along with the immunostained slides were scanned on VENTANA iSCAN HT scanner (Figure 1). Three independent pathologists manually annotated H&E sections to outline the entire tumor region containing invasive cancer ( i.e., core of the tumor [CT]) using a whole tumor section approach (31). They further demarcated the invasive margin (IM) without knowledge of clinical characteristics or outcome by indicating sections of the tumor outline involved in the invasive process.…”
Section: Methodsmentioning
confidence: 99%
“…To address need to work from home during the COVID-19 pandemic, in March 2020 the College of American Pathologists (CAP) issued "COVID-19 Remote Sign-Out Guidance" that stated pathologists "may use a non-FDA approved system as long as it has been properly validated" for primary diagnosis (Borowsky et al, 2020). This statement also has implications for remote biomarker assessment, because although several digital tools were being used for this purpose (Barnes et al, 2017;Pantanowitz et al, 2020) experience with this technology was still limited.…”
Section: Validationmentioning
confidence: 99%
“…As tumors frequently harbor significant cellular and spatial heterogeneity (e.g., stroma, tumor-stroma interface, intratumoral), in particular for immune markers such as PD-L1 or CD8 infiltrates [95], it is essential to perform high-resolution multiplexed analysis across whole tumor sections. It has been demonstrate that the analysis of small ROIs generates significant variation and errors in the assessment of tumor and immune markers in cancer [96,97]. Hence, there is a need for integrated mIHC systems enabling high-degree of multiplexing coupled with digital analysis for high-resolution analyses on whole tumor slides [98].…”
Section: Advantages and Current Limitations Of Multiplexed Immunohmentioning
confidence: 99%