Why the move to microfluidics for protein analysis?

Lion, Niels; Reymond, Frédéric; Girault, Hubert H.; Rossier, Joël S.

doi:10.1016/j.copbio.2004.01.001

Cited by 106 publications

(87 citation statements)

References 49 publications

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“…For example, analytical separations such as capillary electrophoresis (CE) [3,4], two-dimensional electrokinetic separations [5], liquid chromatography (LC) [6], reaction and detection microchambers [7][8][9] have been implemented in the microfluidic format. More recently, analytical microfluidic systems have caught interest in the particular field of proteomics [10,11] because of the high analytical constraints demanded by this field, namely the low amount of sample usually available, the high complexity of the initial protein mixture, and the need for high-throughput analysis [12]. The potential success of analytical systems also lies in the low production costs, opening the way for single use and contamination-free analytical devices.…”

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confidence: 99%

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Roussel

Dayon

Lion

et al. 2004

J. Am. Soc. Mass Spectrom.

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We present herein a review of our work on the on-line electrochemical generation of mass tags toward cysteine residues in peptides and proteins. Taking advantage of the inherent electrochemical nature of electrospray generated from a microfabricated microspray emitter, selective probes for cysteine were developed and tested for on-line nonquantitative mass tagging of peptides and proteins. The nonquantitative aspect of the covalent tagging thus allows direct counting of free cysteines in the mass spectrum of a biomolecule through additional adduct peaks. Several substituted hydroquinones were investigated in terms of electrochemical properties, and their usefulness for on-line mass tagging during microspray experiments were assessed with L-cysteine, peptides, and intact proteins. Complementarily, numerical simulations were performed to properly understand the respective roles of mass transport, kinetics of electrochemical-chemical reactions, and design of the microspray emitter in the mass tagging overall efficiency. Finally, the on-line electrochemical tagging of cysteine residues was applied to the analysis of tryptic peptides of purified model proteins for protein identification through peptide mass fingerprinting. (J Am Soc Mass Spectrom 2004, 15, 1767-1779

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confidence: 99%

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Roussel

Dayon

Lion

et al. 2004

J. Am. Soc. Mass Spectrom.

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“…Among various miRNA detection methods, microfluidic chip-based methods meet the requirements other than portability. The chip itself is small and enables a short detection time and a small required sample volume due to its microscale channel structure; 14 however, relatively large external power sources for fluid pumping are needed and they prevent portability of the microfluidic chip. Hence, we utilized our power-free microfluidic chip driven by energy that is stored in degassed poly(dimethylsiloxane) (PDMS) to overcome this drawback because it eliminates the need for external power sources for fluid pumping.…”

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Multiplex MicroRNA Detection on a Power-free Microfluidic Chip with Laminar Flow-assisted Dendritic Amplification

et al. 2015

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MicroRNA (miRNA) profile-based point-of-care (POC) diagnostic methods have attracted considerable attention. In our laboratory, singleplex miRNA detection on a power-free poly(dimethylsiloxane) (PDMS) microfluidic chip with laminar flow-assisted dendritic amplification (LFDA) has been developed. In this study, to obtain the miRNA profile and to improve the reliability of the diagnosis, multiplex miRNA detection on the same system is demonstrated without compromising any advantages of the singleplex miRNA detection. The limit of detection (LOD) was at the femto-to picomolar level and the assay time was 20 min. The sensitivity, rapidity, and portability of the microfluidic chip are adequate for POC diagnosis.

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“…Winzor and Chan and Chen highlight the biosensor's contributions in affinity chromatography [93] and characterization of protein-fouled membranes [11]. Brandt and Hoheisel, Campàs and Katakis, Disney and Seeberger, Espina et al, Michaud, Pavlivkova et al, and Walsh and Chang each discuss the applicability of SPR as a detection method in the development of higherthroughput protein, carbohydrate, DNA and small molecule array platforms [6,8,24,27,59,68,90], Lion et al summarizes the importance of microfluidics in Biacore biosensors [49], and Buijs and Natsume, Damle et al, Mattei et al and Stuhler and Meyer describe coupling the biosensor with mass spectrometry to streamline isolation and identification of bound analytes from crude materials [7,18,57,84].…”

Section: Reviews Theory and Methodsmentioning

confidence: 99%

Survey of the year 2004 commercial optical biosensor literature

Rich

Myszka

2005

J of Molecular Recognition

115

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The year 2004 represents a milestone for the biosensor research community: in this year, over 1000 articles were published describing experiments performed using commercially available systems. The 1038 papers we found represent a $ 10% increase over the past year and demonstrate that the implementation of biosensors continues to expand at a healthy pace. We evaluated the data presented in each paper and compiled a 'top 10' list. These 10 articles, which we recommend every biosensor user reads, describe wellperformed kinetic, equilibrium and qualitative/screening studies, provide comparisons between binding parameters obtained from different biosensor users, as well as from biosensor-and solution-based interaction analyses, and summarize the cutting-edge applications of the technology. We also re-iterate some of the experimental pitfalls that lead to sub-optimal data and over-interpreted results. We are hopeful that the biosensor community, by applying the hints we outline, will obtain data on a par with that presented in the 10 spotlighted articles. This will ensure that the scientific community at large can be confident in the data we report from optical biosensors.

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Why the move to microfluidics for protein analysis?

Cited by 106 publications

References 49 publications

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Generation of mass tags by the inherent electrochemistry of electrospray for protein mass spectrometry

Multiplex MicroRNA Detection on a Power-free Microfluidic Chip with Laminar Flow-assisted Dendritic Amplification

Survey of the year 2004 commercial optical biosensor literature

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