Wickerhamomyces sinyiensis f.a., sp. nov., a new ascomycetous yeast species in the Wickerhamomyces clade isolated in Taiwan

Abstract

This study describes Wickerhamomyces sinyiensis, a new anamorphic ascomycetous yeast species, four strains of which were isolated from soil and the fruiting body of a mushroom in Taiwan between 2006 and 2007. Analysis of the sequences of the large-subunit rRNA, small-subunit rRNA and elongation factor-1α identified this species as a member of the Wickerhamomyces clade. The yeast strains of W. sinyiensis exhibited a 0–3 nucleotide difference in the sequences of the D1/D2 domain of the large subunit rRNA when compared to one another and a 10 and 11 nucleotide difference when compared to Candida sp. BG99-11-14-10-4-1 and NRRL Y-7574, the closest undescribed species, respectively. The yeast strains differed by 77 and 78 nucleotides from W. orientalis and W. bispora, the close Wickerhamomyces species, respectively. The internal transcribed spacer sequences of the four isolates exhibited a divergence of 106–108 substitutions from the recognized species W. xylosivorus. No sexual reproduction was observed. The strains differed from those of related species in terms of their carbon and nitrogen assimilation patterns. Therefore, this study proposes W. sinyiensis f.a., sp. nov. to accommodate these four strains, with W. sinyiensis BCRC 23185T (isotype CBS 11432T; MycoBank number MB563484) as the holotype.

Wickerhamomyces corioli f.a., sp. nov., a novel yeast species discovered in two mushroom species

“…Notably, certain members of this genus have been isolated from soil [7, 9, 23], seawater [4], pickles and jams [26]. An intriguing addition to this ecological spectrum was made by Chang et al ., who isolated a strain of Wickerhamomyces sinyiensis from the fruiting body of a mushroom [7]. Our current research further demonstrates that species of the genus Wickehamomyces can be isolated from mushrooms.…”

“…Additionally, some have been found in the company of other animals, like in the cecum of cattle [25] and among migratory birds [21]. Notably, certain members of this genus have been isolated from soil [7,9,23], seawater [4], pickles and jams [26]. An intriguing addition to this ecological spectrum was made by Chang et al, who isolated a strain of Wickerhamomyces sinyiensis from the fruiting body of a mushroom [7].…”

“…Species of the genus Wickerhamomyces are also ecologically diverse. They have been isolated from the plant material, insects and other animals, soil, seawater and fermentation environments [4–26]. Despite this ecological diversity, species of the genus Wickerhamomyces isolated from mushrooms are relatively rare, with only one known strain obtained previously [7].…”

“…They have been isolated from the plant material, insects and other animals, soil, seawater and fermentation environments [4–26]. Despite this ecological diversity, species of the genus Wickerhamomyces isolated from mushrooms are relatively rare, with only one known strain obtained previously [7]. However, during a recent survey of yeast diversity associated with mushrooms in PR China, strains 19-39-3 and 19-40-2, isolated from Trametes versicolor (synonymy: Coriolus versicolor ) and Marasmius siccus , respectively, were identified as representing an undescribed species within the genus Wickerhamomyces on the basis of the results of analysis of D1/D2 LSU, SSU, internal transcribed spacer (ITS) and TEF1 sequences, as well as phenotypic comparisons.…”

“…Species of the genus Wickerhamomyces exhibit a global distribution, with isolates hailing from diverse regions such as PR China (Da Hinggan Mountains, Xishuangbanna of Yunnan, Nanyang and Henan Province) [4][5][6], Taiwan [7], Thailand [8][9][10][11], Korea (Ochang) [12], the USA (New Hampshire and Illinois) [13], Italy (Vincence, Sicily and Ustica Island) [13,14], India [13,15], Canada [13], Japan (Iwate Prefecture, Hokkaido) [16,17], Argentina [18,19], North Brazil [20], Ecuador [21], French Guiana [22], Iran [23], South Africa, Austria, Slovakia and others [13]. Species of the genus Wickerhamomyces are also ecologically diverse.…”