Wide-field calcium imaging reveals widespread changes in cortical functional connectivity following mild traumatic brain injury in the mouse

Cramer, Samuel W.; Haley, Samuel P; Popa, Laurentiu S.; Carter, Russell E.; Scott, Earl Parker; Flaherty, Evelyn B; Dominguez, Judith; Sabal, Luke; Surinach, Daniel; Chen, Clark C.; Kodandaramaiah, Suhasa B.; Ebner, Timothy J.

doi:10.1016/j.nbd.2022.105943

Cited by 19 publications

(20 citation statements)

References 83 publications

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“…Thy1-GCaMP6f mouse lines express GCaMP6f 75 in layers II/III and V cortical excitatory pyramidal neurons 76 , allowing for wide-field Ca 2+ fluorescence imaging of predominantly layer II/III excitatory neurons 77 . Mice were surgically implanted with See-Shell polymer windows over the dorsal cerebral cortex 78 as described previously 6,79,80 . Mice were housed in a 12hr-12hr light-dark cycle, with times of darkness (the period of mouse wakefulness) occurring during the day when experiments were performed.…”

Section: Animals and Surgical Setupmentioning

confidence: 99%

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Wide-field calcium imaging of cortical activation and functional connectivity in externally- and internally-driven locomotion

West

Gerhart

Ebner

2023

Preprint

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The neural dynamics underlying self-initiated versus sensory driven movements is central to understanding volitional action. Upstream motor cortices are associated with the generation of internally-driven movements over externally-driven. Here we directly compare cortical dynamics during internally- versus externally-driven locomotion using wide-field Ca2+ imaging. We find that secondary motor cortex (M2) plays a larger role in internally-driven spontaneous locomotion transitions, with increased M2 functional connectivity during starting and stopping than in the externally-driven, motorized treadmill locomotion. This is not the case in steady-state walk. In addition, motorized treadmill and spontaneous locomotion are characterized by markedly different patterns of cortical activation and functional connectivity at the different behavior periods. Furthermore, the patterns of fluorescence activation and connectivity are uncorrelated. These experiments reveal widespread and striking differences in the cortical control of internally- and externally-driven locomotion, with M2 playing a major role in the preparation and execution of the self-initiated state.

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Section: Animals and Surgical Setupmentioning

confidence: 99%

“…All fluorescence imaging was performed with dual-wavelength illumination to minimize the effects of hemodynamics on the Ca 2+ signal 6,28,79,80 . Briefly, mice were head-fixed beneath the fluorescence microscope on the motorized treadmill or freely moving disk (Figure 1A) for imaging of the dorsal cerebral cortex (Figure 1B).…”

Section: Fluorescence Imagingmentioning

confidence: 99%

Wide-field calcium imaging of cortical activation and functional connectivity in externally- and internally-driven locomotion

West

Gerhart

Ebner

2023

Preprint

Self Cite

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“…During most experimental paradigms, the access to real connectivity levels of neurons in networks is extremely limited. Thus, a common practice is to evaluate the functional connectivity by measurements of pairwise correlations between neuronal signals for various signals such as functional magnetic resonance imaging (fMRI) 54,55 , calcium imaging or single unit recordings 56 where configurational structure of the network is compared across time or across subjects through centrality measures such as degree [57][58][59][60][61] . In this work we use a similar practice, where we evaluate the functional connectivity between individual neurons using the correlation matrix, calculated per trial.…”

Section: Layer 2-3 Network Organization In M1 During Motor Learningmentioning

confidence: 99%

VTA projections to M1 are essential for reorganization of layer 2-3 network dynamics underlying motor learning

Ghanayim,

Benisty,

Cohen-Rimon

et al. 2023

Preprint

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SummaryThe primary motor cortex (M1) is crucial for motor skill learning. Previous studies demonstrated that skill acquisition requires dopaminergic VTA (ventral-tegmental area) signaling in M1, however little is known regarding the effect of these inputs at the neuronal and network levels. Using dexterity task, calcium imaging, chemogenetic silencing, and geometric data analysis, we demonstrate VTA-dependent reorganization of M1 layer 2-3 during motor learning. While average activity and average functional connectivity of layer 2-3 network remain stable during learning, the activity kinetics, the correlational configuration of functional connectivity, and average connectivity strength of layer 2-3 neurons gradually transform towards an expert configuration. In addition, task success-failure outcome signaling gradually emerges. Silencing VTA dopaminergic inputs to M1 during learning, prevents all these changes. Our findings demonstrate dopaminergic VTA-dependent formation of outcome signaling and new connectivity configuration of the layer 2-3 network, supporting reorganization of the M1 network for storing new motor skills.

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“…Simultaneous neural computations occurring in many brain regions, and the interactions between these regions, mediate behavior [1][2][3]. Disruptions to these interactions are an indicator of neurological disorders such as Parkinson's disease [4] and traumatic brain injury [5]. Minimally invasive electrocorticography (ECoG) electrode arrays have been used to measure surface field potential generated by neural activity from several distributed populations of neurons in the cortex [6].…”

Section: Introductionmentioning

confidence: 99%

Fully desktop fabricated flexible graphene electrocorticography (ECoG) arrays

Hu¹,

Hossain²,

Navabi³

et al. 2023

J. Neural Eng.

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Flexible Electrocorticography (ECoG) electrode arrays that conform to the cortical surface and record surface field potentials from multiple brain regions provide unique insights into how computations occurring in distributed brain regions mediate behavior. Specialized microfabrication methods are required to produce flexible ECoG devices with high-density electrode arrays. However, these fabrication methods are challenging for scientists without access to cleanroom fabrication equipment. Here we present a fully desktop fabricated flexible graphene ECoG array. First, we synthesized a stable, conductive ink via liquid exfoliation of Graphene in Cyrene. Next, we established a stencil-printing process for patterning the graphene ink via laser-cut stencils on flexible polyimide substrates. Benchtop tests indicate that the graphene electrodes have good conductivity of ~ 1.1 × 103 S·cm-1, flexibility to maintain their electrical connection under static bending, and electrochemical stability in a 15-day accelerated corrosion test. Chronically implanted graphene ECoG devices remain fully functional for up to 180 days, with average in vivo impedances of 24.72 ± 95.23 kΩ at 1 kHz. The ECoG device can measure spontaneous surface field potentials from mice under awake and anesthetized states and sensory stimulus-evoked responses. The stencil-printing fabrication process can be used to create Graphene ECoG devices with customized electrode layouts within 24 hours using commonly available laboratory equipment.

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Wide-field calcium imaging reveals widespread changes in cortical functional connectivity following mild traumatic brain injury in the mouse

Cited by 19 publications

References 83 publications

Wide-field calcium imaging of cortical activation and functional connectivity in externally- and internally-driven locomotion

Wide-field calcium imaging of cortical activation and functional connectivity in externally- and internally-driven locomotion

VTA projections to M1 are essential for reorganization of layer 2-3 network dynamics underlying motor learning

Fully desktop fabricated flexible graphene electrocorticography (ECoG) arrays

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