Wide-Field Optical Imaging in Mouse Models of Ischemic Stroke

Padawer-Curry, Jonah A.; Bowen, Ryan M.; Jarang, Anmol; Wang, Xiaodan; J, Lee; Bauer, Adam Q.

doi:10.1007/978-1-0716-2926-0_11

Cited by 6 publications

(13 citation statements)

References 140 publications

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“…Before drug administration, global resting-state calcium signals exhibited one-over-f like spectra ( Fig. 4a ), in accordance with previous studies [68, 73, 96, 116]. Following saline, calcium power decreased above 0.08 Hz ( Fig.…”

Section: Resultssupporting

confidence: 91%

“…Slow trends in channel-specific light levels were temporally detrended with a 5 th order polynomial fit [68, 70–73, 89–91, 95, 96]. The images were spatially smoothed using a Gaussian filter (5×5 pixel kernel, standard deviation of 1.2 pixels).…”

Section: Discussionmentioning

confidence: 99%

“…Measures of resting-state functional connectivity (RSFC) were calculated using zero lag correlation for all pixel pairs within our field of view, as in previous reports [68, 70, 89, 91, 96]. Briefly, spontaneous activity was filtered over infraslow and delta bands.…”

Section: Discussionmentioning

confidence: 99%

“…These markers were then used to spatially coregistered the FOVs of the cameras using a projective transform. Particularly important for fluorescence imaging [93], dark frames from each camera were averaged and subtracted from that camera's image sequence. All image sequences were then affine transformed and aligned to the Paxinos atlas [94].…”

Section: 2) Neuroimaging Spatial Normalizationmentioning

confidence: 99%

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Psychedelic 5-HT2A receptor agonism: neuronal signatures and altered neurovascular coupling.

Padawer-Curry,

Krentzman,

Kuo

et al. 2023

Preprint

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Psychedelics are attractive potential therapeutic agents for treatment resistant depression due to their rapid and sustained effects. These effects have been largely attributed to serotonin (5-hydroxytryptamine) receptor agonism, especially at the 5-HT2A receptor (5-HT2AR). Recent human functional neuroimaging studies report dramatic 5-HT2AR-dependent changes in behavior and functional network organization that presumably reflect neuromodulatory effects. However, serotonin also has potent vasoactive effects that have not been assessed in prior imaging studies of classic (i.e., 5-HT2AR agonists) psychedelics. We assessed the neural, hemodynamic, and neurovascular coupling (NVC) effects of the hallucinogenic 5-HT2A receptor agonist, 2,5-Dimethoxy-4-iodoamphetamine (DOI), using wide-field optical imaging (WFOI) in mice during both awake, whisker stimulation and under awake, resting-state conditions. Under both task and resting-state conditions, DOI narrowed hemodynamic response functions and enhanced transduction of neuronal activity in the delta band. These effects were regionally specific and strongest in association regions. Co-administration of DOI and 5-HT2aR antagonist, MDL100907, reversed the effects of DOI on NVC. These DOI-induced NVC alterations imply a dissociation between neuronal versus hemodynamic signals and emphasize the need to consider the neurovascular effects of psychedelics when interpreting hemodynamic measures of neuronal activity.

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Section: Resultssupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: 2) Neuroimaging Spatial Normalizationmentioning

confidence: 99%

See 2 more Smart Citations

Psychedelic 5-HT2A receptor agonism: neuronal signatures and altered neurovascular coupling.

Padawer-Curry,

Krentzman,

Kuo

et al. 2023

Preprint

Self Cite

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“…One of the first studies to demonstrate the importance of integration was by Guo and colleagues, who established the necessity of serial information flow from sensory to motor areas for perceptual decision-making 2 . The propagation from sensory regions to the entire dorsal cortex was then explored by several groups using wide-field imaging 1,5–8 . Notably, a strong dependence of the evoked spatiotemporal dynamics on the brain state was demonstrated by comparing wakefulness and anesthesia 9–12 .…”

Section: Introductionmentioning

confidence: 99%

Cortical mapping of sensory responses reveals strong brain-state dependence of the late component

Montagni,

Resta,

Tort-Colet

et al. 2023

Preprint

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Sensory information must be integrated across a distributed brain network for stimulus processing and perception. Recent studies have revealed specific spatiotemporal patterns of cortical activation for the early and late components of sensory-evoked responses, which are associated with stimulus features and perception, respectively. However, our understanding of how the brain state influences the sensory-evoked activation across the mouse cortex remains limited. In this study, we utilized isoflurane to modulate the brain state and conducted wide-field calcium imaging of Thy1-GCaMP6f mice to monitor the distributed activation evoked by multi-whisker stimulation. Our findings reveal that the level of anesthesia strongly shapes the spatiotemporal features and the functional connectivity of the sensory-activated network. As anesthesia levels decrease, we observe increasingly complex responses, accompanied by the emergence of the late component within the sensory-evoked response. The persistence of the late component under anesthesia raises new questions regarding the potential existence of perception during unconscious states.

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