Wide-scope analysis of pesticide and veterinary drug residues in meat matrices by high resolution MS: detection and identification using Exactive-Orbitrap

Abstract

A multiresidue and multiclass method for the simultaneous determination of more than 350 compounds including pesticides, biopesticides and veterinary drugs in different meat matrices (beef, pork and chicken) by ultra-high performance liquid chromatography coupled to Orbitrap MS has been developed. In the present study, the determination of fragments was accomplished as an essential tool for a reliable identification of compounds using high resolution MS. To obtain these fragments, different strategies have been carried out in order to ensure an appropriate fragment assignment and identification. The analytical method is suitable for qualitative analysis, and it was also evaluated for quantitative analysis. Generic extraction conditions were optimized, obtaining adequate recovery and precision values for most of the studied analytes (>290). The limits of detection ranged from 2 to 16 µg kg(-1). Limits of quantification were 10 µg kg(-1) with the exception of few compounds with a higher value (50 or 100 µg kg(-1)). Limits of identification were also established, and they ranged from 2 to 150 µg kg(-1). This method was applied to the analysis of 18 meat samples and some veterinary drugs as enrofloxacin and sulfadiazine were detected and further identified/quantified (with triple quadrupole) in two different samples at 33 µg kg(-1) and trace levels, respectively. No pesticides were detected in the analyzed samples.

Comprehensive analysis of toxics (pesticides, veterinary drugs and mycotoxins) in food by UHPLC-MS

“…In this case, the use of UHPLC provided symmetrical and narrow peaks (4-9 s peak width). The same analyzer, Exactive-Orbitrap, was used by Gómez-Pérez et al for the determination of more than 350 compounds, including pesticides and veterinary drugs in honey and meat [71,72]. In this case, generic chromatographic and MS conditions were used because of the wide range of physicochemical characteristics of the compounds.…”

Multiresidue Method for the Rapid Determination of Pesticide Residues in Tea Using Ultra Performance Liquid Chromatography Orbitrap High Resolution Mass Spectrometry and In-Syringe Dispersive Solid Phase Extraction

“…LODs were estimated analyzing three blank samples spiked at 0.5, 1.0, 2.0, 5.0, and 10.0 μg kg –1 . They are defined as the minimum concentration at which the accurate mass error was ±5 ppm, 42 and LODs were less than or equal to 5 μg kg –1 for all pesticides except dimetachlone at 10 μg kg –1 (see Table S-3 ). According to accuracy and precision measurement, LOQs were 10 μg kg –1 for 104 pesticides which provided recoveries between 70% and 130%, and RSDs below 20%.…”

Future perspectives in Orbitrap™-high-resolution mass spectrometry in food analysis: a review

“…Screening has also been carried out to simultaneously monitor both veterinary drug and pesticide residues in honey (Gomez-Perez et al, 2012), meat (Gomez-Perez et al, 2014) and baby food (Gomez-Perez et al, 2015). A generic extraction was employed for honey involving dilution with water, adding acetonitrile/formic acid, shaking, centrifugation and direct injection of the extract, and a similar approach was employed with homogenised meat and with meat, fish and vegetable-based baby foods.…”