Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution

Corsetti, Stella; Wijesinghe, Philip; Poulton, Persephone Beatrice; Sakata, Shuzo; Vyas, Kushi; Herrington, C. Simon; Nylk, Jonathan; Gasparoli, Federico M.; Dholakia, Kishan

doi:10.1364/osac.391644

Cited by 17 publications

(14 citation statements)

References 28 publications

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“…So-called ghost imaging can be one of such application areas [248]. The DL techniques can also pave the way to reach super-resolution in THz imaging as it evolves in widefield light sheet microscopy, for instance, using an Airy beam [437] or to remove non-resonant background as it was done in CARS experiments [438].…”

Section: Artificial Intelligence In Thz Imagingmentioning

confidence: 99%

Roadmap of Terahertz Imaging 2021

Valušis

Lisauskas

Yuan

et al. 2021

Sensors

191

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In this roadmap article, we have focused on the most recent advances in terahertz (THz) imaging with particular attention paid to the optimization and miniaturization of the THz imaging systems. Such systems entail enhanced functionality, reduced power consumption, and increased convenience, thus being geared toward the implementation of THz imaging systems in real operational conditions. The article will touch upon the advanced solid-state-based THz imaging systems, including room temperature THz sensors and arrays, as well as their on-chip integration with diffractive THz optical components. We will cover the current-state of compact room temperature THz emission sources, both optolectronic and electrically driven; particular emphasis is attributed to the beam-forming role in THz imaging, THz holography and spatial filtering, THz nano-imaging, and computational imaging. A number of advanced THz techniques, such as light-field THz imaging, homodyne spectroscopy, and phase sensitive spectrometry, THz modulated continuous wave imaging, room temperature THz frequency combs, and passive THz imaging, as well as the use of artificial intelligence in THz data processing and optics development, will be reviewed. This roadmap presents a structured snapshot of current advances in THz imaging as of 2021 and provides an opinion on contemporary scientific and technological challenges in this field, as well as extrapolations of possible further evolution in THz imaging.

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Section: Artificial Intelligence In Thz Imagingmentioning

confidence: 99%

Roadmap of Terahertz Imaging 2021

Valušis

Lisauskas

Yuan

et al. 2021

Sensors

191

View full text Add to dashboard Cite

show abstract

“…Remarkable, deep learning approach has become a promising method to resolve above problems and is applied in LSM to enhance the resolution and discover intricate structures (Wagner et al, 2021). Dholakia's group combined the use of a non-diffracting Airy light field and a deep-learning method to demonstrate an open-top light sheet microscopy (Figure 4D) (Corsetti et al, 2020). The adoption of deeplearning in their system has demonstrated a near two-fold improvement in resolution whilst maintaining a wide field of view for light sheet imaging.…”

Section: Lattice Light Sheet Microscopymentioning

confidence: 99%

“…Reprinted from Liu et al (2018) with permission from American Association for the Advancement of Science. (D) Schematic of the setup of LSM using an Airy beam ( Corsetti et al, 2020 ). Reprinted from Corsetti et al (2020) with permission from Optical Society of America.…”

Section: Super-resolution Techniques For In Vivo Imagingmentioning

confidence: 99%

Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging

Jing

Zhang

et al. 2021

Front. Chem.

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Over the past two decades, super-resolution microscopy (SRM), which offered a significant improvement in resolution over conventional light microscopy, has become a powerful tool to visualize biological activities in both fixed and living cells. However, completely understanding biological processes requires studying cells in a physiological context at high spatiotemporal resolution. Recently, SRM has showcased its ability to observe the detailed structures and dynamics in living species. Here we summarized recent technical advancements in SRM that have been successfully applied to in vivo imaging. Then, improvements in the labeling strategies are discussed together with the spectroscopic and chemical demands of the fluorophores. Finally, we broadly reviewed the current applications for super-resolution techniques in living species and highlighted some inherent challenges faced in this emerging field. We hope that this review could serve as an ideal reference for researchers as well as beginners in the relevant field of in vivo super resolution imaging.

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“…The research data underpinning this publication can be accessed at https:// doi.org/10.17630/7cee889f-aa36-4c27-a485-262c8a5d336b [30].…”

Section: Discussionmentioning

confidence: 99%

Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution

Corsetti

Wijesinghe

Poulton

et al. 2020

Preprint

Self Cite

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AbstractImaging across length scales and in depth has been an important pursuit of widefield optical imaging. This promises to reveal fine cellular detail within a widefield snapshot of a tissue sample. Current advances often sacrifice resolution through selective sub-sampling to provide a wide field of view in a reasonable time scale. We demonstrate a new avenue for recovering high-resolution images from sub-sampled data in light-sheet microscopy using deep-learning super-resolution. We combine this with the use of a widefield Airy beam to achieve high-resolution imaging over extended fields of view and depths. We characterise our method on fluorescent beads as test targets. We then demonstrate improvements in imaging amyloid plaques in a cleared brain from a mouse model of Alzheimer’s disease, and in excised healthy and cancerous colon and breast tissues. This development can be widely applied in all forms of light sheet microscopy to provide a two-fold increase in the dynamic range of the imaged length scale. It has the potential to provide further insight into neuroscience, developmental biology and histopathology.

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Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution

Cited by 17 publications

References 28 publications

Roadmap of Terahertz Imaging 2021

Roadmap of Terahertz Imaging 2021

Super-Resolution Microscopy: Shedding New Light on In Vivo Imaging

Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution

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