Widefield phototransient imaging for visualizing 3D motion of resonant particles in scattering environments

Liebel, Matz; Camargo, Franco V. A.; Cerullo, Giulio; Hulst, Niek F. van

doi:10.1039/d1nr06837g

Cited by 6 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our single-particle and absorption-based measurements revealed g factors that are 1 order of magnitude larger than the ones found by ensemble extinction-based studies. , The images presented in Figure were obtained by scanning the sample with a piezo-stage and thus require long image acquisition times. Recent advances in the field of wide-field PT imaging, − which use cameras instead of confocal scanning, could open the possibility for faster image acquisition with magnetic contrast. PT imaging is particularly well suited for studying the absorption of small particles because the absorption scales with the volume.…”

Section: Discussionmentioning

confidence: 99%

Imaging the Magnetization of Single Magnetite Nanoparticle Clusters via Photothermal Circular Dichroism

et al. 2022

View full text Add to dashboard Cite

Magnetic imaging is a versatile tool in biological and condensed-matter physics. Existing magnetic imaging techniques either require demanding experimental conditions which restrict the range of their applications or lack the spatial resolution required for single-particle measurements. Here, we combine photothermal (PT) microscopy with magnetic circular dichroism (MCD) to develop a versatile magnetic imaging technique using visible light. Unlike most magnetic imaging techniques, photothermal magnetic circular dichroism (PT MCD) microscopy works particularly well for single nanoparticles immersed in liquids. As a proof of principle, we demonstrate magnetic CD imaging of superparamagnetic magnetite nanoparticulate clusters immersed in microscope immersion oil. The sensitivity of our method allowed us to probe the magnetization curve of single ∼400-nm-diameter magnetite nanoparticulate clusters.

show abstract

Section: Discussionmentioning

confidence: 99%

Imaging the Magnetization of Single Magnetite Nanoparticle Clusters via Photothermal Circular Dichroism

et al. 2022

View full text Add to dashboard Cite

show abstract

“…By using a recently developed holographic transient microscope, which allows a reliable discrimination between non-resonant dielectric and resonant metallic nanoparticles, the same group demonstrated an ultrafast identification, visualization and tracking of unlabeled nanoparticles in the presence of nonspecific scattering, which is thus potentially applicable to living cells and tissues. 113 , 114 …”

Section: Imaging Techniquesmentioning

confidence: 99%

Optical Microscopy Systems for the Detection of Unlabeled Nanoparticles

Friedrich¹,

Kappes²,

Cicha³

et al. 2022

IJN

View full text Add to dashboard Cite

Label-free detection of nanoparticles is essential for a thorough evaluation of their cellular effects. In particular, nanoparticles intended for medical applications must be carefully analyzed in terms of their interactions with cells, tissues, and organs. Since the labeling causes a strong change in the physicochemical properties and thus also alters the interactions of the particles with the surrounding tissue, the use of fluorescently labeled particles is inadequate to characterize the effects of unlabeled particles. Further, labeling may affect cellular uptake and biocompatibility of nanoparticles. Thus, label-free techniques have been recently developed and implemented to ensure a reliable characterization of nanoparticles. This review provides an overview of frequently used label-free visualization techniques and highlights recent studies on the development and usage of microscopy systems based on reflectance, darkfield, differential interference contrast, optical coherence, photothermal, holographic, photoacoustic, total internal reflection, surface plasmon resonance, Rayleigh light scattering, hyperspectral and reflectance structured illumination imaging. Using these imaging modalities, there is a strong enhancement in the reliability of experiments concerning cellular uptake and biocompatibility of nanoparticles, which is crucial for preclinical evaluations and future medical applications.

show abstract

Ultrabroadband Optical Diffraction Tomography

Hörmann,

Camargo,

van Hulst

et al. 2024

ACS Photonics

View full text Add to dashboard Cite

Optical diffraction tomography (ODT) is a powerful noninvasive 3D imaging technique, but its combination with broadband light sources is difficult. In this study, we introduce ultrabroadband ODT, covering over 150 nm of visible spectral bandwidth with a lateral spatial resolution of 150 nm. Our work addresses a critical experimental gap by enabling the measurement of broadband refractive index changes in 3D samples, crucial information that is difficult to assess with existing methodologies. We present broadband, spectrally resolved ODT images of HeLa cells, obtained via pulse-shaping-based Fourier transform spectroscopy. The spectral observations enabled by ultrabroadband ODT, combined with material-dependent refractive index responses, allow for precise three-dimensional identification of nanoparticles within cellular structures. Our work represents a crucial step toward time and spectrally resolved tomography of complex 3D structures with implications for life and materials science applications.

show abstract

Widefield phototransient imaging for visualizing 3D motion of resonant particles in scattering environments

Abstract: We show how ultrafast holographic transient microscopy can be used to identify, visualise and 3D track dynamically moving non-fluorescent nanoparticles in large volumes-of-view and in the presence of non-specific scattering background.

Cited by 6 publications

References 26 publications

Imaging the Magnetization of Single Magnetite Nanoparticle Clusters via Photothermal Circular Dichroism

Imaging the Magnetization of Single Magnetite Nanoparticle Clusters via Photothermal Circular Dichroism

Optical Microscopy Systems for the Detection of Unlabeled Nanoparticles

Ultrabroadband Optical Diffraction Tomography

Contact Info

Product

Resources

About