Widely targeted analysis of metabolomic changes of Cucumis sativus induced by cucurbit chlorotic yellows virus

Zhang, Zelong; He, Haifang; Yan, Minghui; Zhao, Chenchen; Lei, Caiyan; Li, Jingjing; Zhou, Huanbin

doi:10.1186/s12870-022-03555-3

Cited by 16 publications

(6 citation statements)

References 60 publications

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“…Recent studies have shown that hop latent viroid affected cannabis inflorescence size as well as plant growth, while beet curly top virus reduced plant growth (Bektaş et al, 2019;Giladi et al, 2020). CCYV effects on metabolic pathways in cucurbits have recently been reported (Zhang et al, 2022). However, the possible effect on the cannabinoid profile in cannabis is yet to be established.…”

Section: F I G U R Ementioning

confidence: 99%

Cucurbit chlorotic yellows virus, a crinivirus infecting Cannabis sativa plants

Gezovitch,

Luria,

Lachman

et al. 2023

Plant Pathology

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High cannabidiol‐containing plants of Cannabis sativa (high‐CBD) growing in farms in Israel displayed foliar symptoms of interveinal chlorosis and yellowing, brittleness and occasionally necrosis. These symptoms, which were more apparent in older leaves, resembled those caused by the crinivirus lettuce chlorosis virus (LCV). However, this virus was not detected by reverse transcription (RT)‐PCR using specific primer sets. High‐throughput sequencing of viral RNA extracted from symptomatic leaves revealed the presence of cucurbit chlorotic yellows virus (CCYV), a crinivirus in the Closteroviridae family. The complete viral genome sequence was obtained using RT‐PCR followed by Sanger sequencing. The two CCYV RNA genomic segments shared 99.5%–99.85% nucleotide sequence identity with CCYV isolates from the GenBank. The virus was transmitted from symptomatic cannabis leaves to healthy plants of cannabis and Cucumis sativus ‘King Star’ (cucumber) by the whitefly Bemisia tabaci Middle Eastern Asia Minor 1 (MEAM1) species, causing disease symptoms identical to those of the donor plants. Cannabis‐CCYV was also transmitted between infected cucumber plants and cannabis seedlings of unknown genotype. Severe disease symptoms of yellowing and leaf‐edge necrosis were observed on high‐CBD and high Δ9‐tetrahydrocannabinol‐containing (high‐THC) flowering cannabis plants and were associated with mixed infections of LCV and CCYV. To the best of our knowledge, this is the first report of CCYV infecting C. sativa plants.

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Section: F I G U R Ementioning

confidence: 99%

Cucurbit chlorotic yellows virus, a crinivirus infecting Cannabis sativa plants

Gezovitch,

Luria,

Lachman

et al. 2023

Plant Pathology

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“…Viral infections are known to cause a range of physiological and metabolic changes in the host plant, which are observed to influence the behavior and fitness of insect vectors as well [ 70 , 71 ]. A number of studies have shown a role of plant volatile emission spectra in the behavior of insect viral vectors and, consequently, in the transmission of the vectored viruses.…”

Section: Crosstalk Between Virus and Insect Transmission Vector: Anti...mentioning

confidence: 99%

Small Talk: On the Possible Role of Trans-Kingdom Small RNAs during Plant–Virus–Vector Tritrophic Communication

Matsumura

Kormelink

2023

Plants

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Small RNAs (sRNAs) are the hallmark and main effectors of RNA silencing and therefore are involved in major biological processes in plants, such as regulation of gene expression, antiviral defense, and plant genome integrity. The mechanisms of sRNA amplification as well as their mobile nature and rapid generation suggest sRNAs as potential key modulators of intercellular and interspecies communication in plant-pathogen–pest interactions. Plant endogenous sRNAs can act in cis to regulate plant innate immunity against pathogens, or in trans to silence pathogens’ messenger RNAs (mRNAs) and impair virulence. Likewise, pathogen-derived sRNAs can act in cis to regulate expression of their own genes and increase virulence towards a plant host, or in trans to silence plant mRNAs and interfere with host defense. In plant viral diseases, virus infection alters the composition and abundance of sRNAs in plant cells, not only by triggering and interfering with the plant RNA silencing antiviral response, which accumulates virus-derived small interfering RNAs (vsiRNAs), but also by modulating plant endogenous sRNAs. Here, we review the current knowledge on the nature and activity of virus-responsive sRNAs during virus–plant interactions and discuss their role in trans-kingdom modulation of virus vectors for the benefit of virus dissemination.

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“…There exists a wealth of research conducted on the altered metabolic state of entire symptomatic plant leaves during virus infection using metabolomics (Choi et al, 2006; Lionel et al, 2012; Mandal et al, 2012; López-Gresa et al, 2012; Palama et al, 2012; Plischke et al, 2012; Manchester and Anand, 2017; Villa-Ruano et al, 2018; Rossouw et al, 2019; Velásquez-Valle et al, 2020; J. Mishra et al, 2020; Maravi et al, 2022; More et al, 2022; Zhang et al, 2022). However, there remains a notable dearth of studies investigating the precise changes in the apoplast and cytoplasm region-specific metabolite composition during viral infection.…”

Section: Introductionmentioning

confidence: 99%

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

Dhobale,

Sahoo

2023

Preprint

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Plant viruses exert control over the host metabolism to enhance their survival, but the specific sites where altered metabolites take effect remain enigmatic. This study focuses on the apoplastic region of symptomatic mungbean leaves infected with mungbean yellow mosaic India virus (MYMIV) to gain new insights into metabolite changes during infection. Leveraging NMR-based metabolome fingerprinting, we identified several stress-associated metabolites. Notably, proline and 2-Hydroxybutyrate were found to be up-regulated in the apoplast region, while down-regulated in the cytosol. Additionally, aspartate was found to be upregulated in the cytosolic region but absent in the apoplast. Importantly, our molecular analysis of the apoplast suggests the presence of MYMIV genomic components. Moreover, we characterized two distinct populations of extracellular vesicles (EVs) using ultracentrifugation, transmission electron microscopy, dynamic light scattering, and fluorometric assays. The data revealed alterations in the size and count of EVs, suggesting their potential role in facilitating the transport of viral components for long-distance cell-to-cell movement during infection. These findings provide valuable insights into the apoplast's role and the significance of EVs in MYMIV infection, contributing to a better understanding of virus-host interactions and potentially informing new virus defense strategies.

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Widely targeted analysis of metabolomic changes of Cucumis sativus induced by cucurbit chlorotic yellows virus

Cited by 16 publications

References 60 publications

Cucurbit chlorotic yellows virus, a crinivirus infecting Cannabis sativa plants

Cucurbit chlorotic yellows virus, a crinivirus infecting Cannabis sativa plants

Small Talk: On the Possible Role of Trans-Kingdom Small RNAs during Plant–Virus–Vector Tritrophic Communication

Identification of Begomovirus Genomic Components and Stress-specific Metabolic Markers in Mungbean Leaf Apoplast

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