Widely Targeted Lipidomics and Transcriptomics Analysis Revealed Changes of Lipid Metabolism in Spleen Dendritic Cells in Shrimp Allergy

Abstract

Shrimp allergy (SA) is pathological type 2 inflammatory immune responses against harmless shrimp protein allergen, which is caused by complex interactions between dendritic cells (DCs) and other immune cells. Lipid metabolism in different DCs states are significantly changed. However, the lipid metabolism of spleen DCs in SA remain ambiguous. In this study, we established a BALB/c mouse shrimp protein extract-induced allergy model to determine the lipid profile of spleen DCs in SA, and the molecular mechanism between lipid metabolism and immune inflammation was preliminarily studied. Spleen DCs were sorted by fluorescence-activated cell sorting, and then widely targeted lipidomics and transcriptomics analysis were performed. Principal component analysis presented the lipidome alterations in SA. The transcriptomic data showed that Prkcg was involved in lipid metabolism, immune system, and inflammatory signaling pathway. In the correlation analysis, the results suggested that Prkcg was positively correlated with triacylglycerol (Pearson correlation coefficient = 0.917, p = 0.01). The lipidomics and transcriptomics integrated pathway analysis indicated the activated metabolic conversion from triacylglycerol to 1,2-diacyl-sn-glycerol and the transmission of lipid metabolism to immune inflammation (from triacylglycerol and ceramide to Prkcg) in SA spleen DCs, and cellular experiments in vitro showed that glyceryl trioleate and C16 ceramide treatment induced immune function alteration in DCs.

Immune Metabolism in TH2 Responses: New Opportunities to Improve Allergy Treatment — Cell Type-Specific Findings (Part 2)

“…Based on a shrimp protein extract-induced BALB/c mouse allergy model, Sun et al reported distinct differences in the lipid profile and metabolism of spleen DCs of mice being sensitized and challenged with shrimp extract [ 9 ]. The authors concluded that lipid metabolism is involved in shrimp allergy and might influence inflammatory immune responses [ 9 ].…”

“…Based on a shrimp protein extract-induced BALB/c mouse allergy model, Sun et al reported distinct differences in the lipid profile and metabolism of spleen DCs of mice being sensitized and challenged with shrimp extract [ 9 ]. The authors concluded that lipid metabolism is involved in shrimp allergy and might influence inflammatory immune responses [ 9 ]. The supplementation of exogenous lipid glyceryl trioleate affected the immune function of DCs by decreasing the expression of the Th2-associated gene Il4 and increasing the Th1-related gene Il12a and therefore improved the Th1/Th2 immune skew [ 9 ].…”

“…The authors concluded that lipid metabolism is involved in shrimp allergy and might influence inflammatory immune responses [ 9 ]. The supplementation of exogenous lipid glyceryl trioleate affected the immune function of DCs by decreasing the expression of the Th2-associated gene Il4 and increasing the Th1-related gene Il12a and therefore improved the Th1/Th2 immune skew [ 9 ]. The C16 lipid ceramide also showed regulatory effects on DC by decreasing the expression of Stat3 and Rela, both being involved in cell signaling pathways that regulate allergic reactions [ 9 ].…”

“…The supplementation of exogenous lipid glyceryl trioleate affected the immune function of DCs by decreasing the expression of the Th2-associated gene Il4 and increasing the Th1-related gene Il12a and therefore improved the Th1/Th2 immune skew [ 9 ]. The C16 lipid ceramide also showed regulatory effects on DC by decreasing the expression of Stat3 and Rela, both being involved in cell signaling pathways that regulate allergic reactions [ 9 ]. Therefore, glyceryl trioleate and C16 ceramide might represent potential candidates for dietary supplementation to reduce shrimp allergy [ 9 ].…”

“…The C16 lipid ceramide also showed regulatory effects on DC by decreasing the expression of Stat3 and Rela, both being involved in cell signaling pathways that regulate allergic reactions [ 9 ]. Therefore, glyceryl trioleate and C16 ceramide might represent potential candidates for dietary supplementation to reduce shrimp allergy [ 9 ].…”