Widely Targeted Metabolomics Analysis ofDendrobium officinaleat Different Altitudes

Abstract

In order to investigate the differences in the chemical composition of Dendrobium officinale at different altitudes, we collected Dendrobium officinale from different altitudes in Guizhou Province, China, and firstly determined its polysaccharide content by using a sulfuric acid-phenol color development method with reference to the Chinese Pharmacopoeia, and further determined its metabolites by using widely targeted metabolomics, and explored the differences in the chemical composition of Dendrobium officinale at different altitudes using multivariate statistical analysis. We found that the polysaccharide content was higher in plants growing at 1122 m, a total of 902 secondary metabolites were detected by wildly targeted metabolomics, and amino acids and their derivatives were more highly expressed at 1122 m, while other metabolites were more highly expressed at 835 m. Furthermore, we found that the phenolic acid compound nerugein was only present in plants at 835 m, and two lipid compounds, namely Lyso PE 20 : 4 and its isomer, were only present at 1122 m. Taken together, these results may provide a basis for the selection and clinical application of D. officinale cultivated at different altitudes.

Integrated widely targeted metabolomics and network pharmacology revealed quality disparities between Guizhou and conventional producing areas of Codonopsis Radix

“…This might be related to the fact that Guizhou's production area is in a region with low longitude, low latitude, high altitude, heavy precipitation, high annual average temperatures, and average relative humidity. Moreover, both species ( 29 , 48 ) and the growing environment have a strong influence on the accumulation of secondary metabolites in plants, and metabolite accumulation can vary to some extent between the same species based on their growing environments ( 27 , 31 ).…”

“…. Sample preparation CR samples were prepared by reference methods (27). Fresh CR samples were vacuum freeze-dried in a freeze-dryer (Scientz-100F, Scientz, China) before being crushed to powder in a blender (30 Hz, 1.5 min, MM 400, Retsch, Germany).…”

“…Metabolomics research has become a hot research topic in the post-genomic era. It has been widely applied to the study of secondary metabolites in medicinal plants such as Dendrobium officinale Kimura et Migo ( 27 ), Pueraria lobata (Willd.) Ohwi ( 28 , 29 ), Curcuma Longa L. ( 30 ), Lycium barbarum L. ( 31 ), Scutellaria baicalensis Georgi ( 32 ), and Sesamum indicum L. ( 33 ).…”

Flavonoid Metabolome-Based Active Ingredient Mining and Callus Induction in Catalpa bungei C. A. Mey

“…The method utilizes Q TRAP mass spectrometry based on the multi-reaction monitoring (MRM) mode, allowing the simultaneous quantification of hundreds to thousands of metabolites with both breadth and high sensitivity [12]. This method has been applied in recent years for the detection and identification of active ingredients in plants [13][14][15]. Xu et al [1] obtained two flavonoids named Luteolin and Apigenin with antioxidant activity via column chromatography and a biological activity tracking method.…”

Effects of the epiphytic patterns on endophytes and metabolites of Dendrobium nobile Lindl

“…The quality of traditional Chinese material has always been an essential topic in traditional Chinese medicine research. Previous studies have revealed that cultivation methods and environmental factors influence the quality of traditional Chinese material ( Lan et al., 2022 ; Jia et al., 2023 ). In the study of the quality of Dendrobium officinale , it was confirmed that the pine bark substrate is the optimal cultivation substrate by comparing the differences in metabolites among three substrates: pine bark, coconut coir, and pine bark ( Zuo et al., 2020 ).…”