Widely Targeted UHPLC-MS/MS Metabolomic Analysis on the Chemical Variation in Blueberry-Filled Pastries During Processing

Zheng, Jie; Zhong-jun, WU; Yang, Nan; Zhou, Kangning; Hu, Wenzhong; Ou, Shiyi; Liu, Pengzhan

doi:10.3389/fnut.2020.569172

Cited by 4 publications

(5 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Introductionmentioning

confidence: 99%

“…Metabolomics is usually applied to qualitative and quantitative analysis of all small molecules (namely, targeted and non-targeted compounds) detected in the sample ( 12 ). Analysis of changes in various chemical components during food processing helps to deepen the understanding of the mechanism of chemical component transformation in food processing ( 12 ). In recent years, metabolomics has also been applied to the study of Cistanche deserticola for the discrimination of different parts ( 11 ) and different Cistanche deserticola species ( 13 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

Zhang

et al. 2021

Front. Nutr.

View full text Add to dashboard Cite

Cistanche deserticola is one of the most precious plants, traditionally as Chinese medicine, and has recently been used in pharmaceutical and healthy food industries. Steaming and drying are two important steps in the processing of Cistanche deserticola. Unfortunately, a comprehensive understanding of the chemical composition changes of Cistanche deserticola during thermal processing is limited. In this study, ultra-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based widely targeted metabolomics analysis was used to investigate the transformation mechanism of Cistanche deserticola active compounds during steaming and drying processes. A total of 776 metabolites were identified in Cistanche deserticola during thermal processing, among which, 77 metabolites were differentially regulated (p < 0.05) wherein 39 were upregulated (UR) and 38 were downregulated (DR). Forty-seven (17 UR, 30 DR) and 30 (22 UR, 8 DR) differential metabolites were identified during steaming and drying, respectively. The most variation of the chemicals was observed during the process of steaming. Metabolic pathway analysis indicated that phenylpropanoid, flavonoid biosynthesis, and alanine metabolism were observed during steaming, while glycine, serine, and threonine metabolism, thiamine metabolism, and unsaturated fatty acid biosynthesis were observed during drying. The possible mechanisms of the chemical alterations during thermal processing were also provided by the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Furthermore, the blackening of the appearance of Cistanche deserticola mainly occurred in the steaming stage rather than the drying stage, which is associated with the metabolism of the amino acids. All results indicated that the formation of active compounds during the processing of Cistanche deserticola mainly occurred in the steaming stage.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

Zhang

et al. 2021

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…With widely targeted metabolomics analysis, thousands of metabolites can be quickly detected and accurately quantified to assess the metabolome underlying the phenotype of organisms and investigate metabolite variability among different organs, varieties, and species ( Scalbert et al., 2011 ). This approach has been widely used in fruit metabolite analysis, including peach ( Gedük and Atsız, 2022 ), kiwifruit ( Li et al., 2023 ), cherry ( Yang et al., 2021 ), and blueberry ( Zheng et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Widely targeted metabolome profiling of different plateau raspberries and berry parts provides innovative insight into their antioxidant activities

Ren

Wang

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Raspberries are highly nutritious and have powerful antioxidant properties, making them functional berries with positive effects on physiological functioning. However, there is limited information available on the diversity and variability of metabolites in raspberry and its parts, especially in plateau raspberries. To address this, commercial raspberries and their pulp and seeds from two plateaus in China were subjected to LC-MS/MS-based metabolomics analysis and evaluated for antioxidant activity using four assays. A metabolite-metabolite correlation network was established based on antioxidant activity and correlation analysis. The results showed that 1661 metabolites were identified and classified into 12 categories, with significant variations in composition between the whole berry and its parts from different plateaus. Flavonoids, amino acids and their derivatives, and phenolic acids were found to be up-regulated in Qinghai’s raspberry compared to Yunnan’s raspberry. The main differently regulated pathways were related to flavonoid, amino acid, and anthocyanin biosynthesis. The antioxidant activity of Qinghai’s raspberry was stronger than Yunnan’s raspberry, and the order of antioxidant capacity was seed > pulp > berry. The highest FRAP (420.31 µM TE/g DW) values was found in the seed of Qinghai’s raspberry. Overall, these findings suggest that the environment in which the berries grow can affect their chemical composition, and comprehensive exploitation and cultivation of whole raspberry and its parts from different plateaus can lead to new opportunities for phytochemical compositions and antioxidant activity.

show abstract

“…Widely targeted metabolomic analysis by ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), possessing accurate and reliable characteristics, has been successfully applied to determine numerous metabolites in diverse organisms (S. J. Zheng, Wu, et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Widely targeted metabolomic analysis by ultra‐performance liquid chromatography‐tandem mass spectrometry (UPLC‐MS/MS), possessing accurate and reliable characteristics, has been successfully applied to determine numerous metabolites in diverse organisms (S. Zheng, Liu, et al, 2020; J. Zheng, Wu, et al, 2020). To reveal taste contributions of multitudinous compounds, UPLC‐MS/MS based metabolomics test was carried out to identify and quantify all metabolites in the two aromatic rice cultivars.…”

Section: Introductionmentioning

confidence: 99%

Identification of key taste components in two aromatic rice varieties by widely targeted metabolomics in China

Zhao

Duan

Hou³

et al. 2022

Cereal Chem

View full text Add to dashboard Cite

Background and Objectives Due to the lack of comprehensive evaluation of all metabolites in rice, the metabolic reasons for taste differences are unclear. Here, two local varieties Wuyoudao 4 (WYD) (rich‐sweet flavor) and Nanjing 9108 (NJ) (delicate‐sweet flavor) were selected for ultra‐performance liquid chromatography‐tandem mass spectrometry based widely targeted metabolomic analysis. Findings WYD and NJ were clearly separated by principal component analysis and hierarchical clustering analysis, and 231 metabolites were different between the cultivars, including 35 amino acids and derivatives, 14 saccharides and alcohols, and 12 organic acids. Among them, glucose, fructose, arabitol, and xylitol, contributed mainly to the rich‐sweet flavor of WYD rice. Leucine, phenylalanine, tryptophan, isocitric acid, and malic acid were the dominant compounds in NJ rice, and therefore, contributed primarily to the delicate‐sweet flavor. Conclusions Vital compounds of rich‐sweet for WYD, and delicate‐sweet for NJ were clear. The differential metabolites were significantly enriched in the glucosinolate biosynthesis, valine, leucine and isoleucine degradation, and 2‐oxocarboxylic acid metabolism pathways. Significance and Novelty This study would provide fundamental insights into the taste formation compounds and mechanism of aromatic rice.

show abstract

Widely Targeted UHPLC-MS/MS Metabolomic Analysis on the Chemical Variation in Blueberry-Filled Pastries During Processing

Cited by 4 publications

References 44 publications

Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

Widely Targeted Metabolomics Analysis to Reveal Transformation Mechanism of Cistanche Deserticola Active Compounds During Steaming and Drying Processes

Widely targeted metabolome profiling of different plateau raspberries and berry parts provides innovative insight into their antioxidant activities

Identification of key taste components in two aromatic rice varieties by widely targeted metabolomics in China

Contact Info

Product

Resources

About