Widening the Gene Pool of Sexual Tetraploid Bahiagrass: Generation and Reproductive Characterization of a Sexual Synthetic Tetraploid Population

Zilli, Alex Leonel; Acuña, Carlos Alberto; Schulz, Roberto R.; Brugnoli, Elsa Andrea; Guidalevich, Veronica; Quarín, Camilo Luis; Martínez, Eric Javier

doi:10.2135/cropsci2017.07.0457

Cited by 24 publications

(49 citation statements)

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“…Genetic variability is the key for planning and implementing breeding programmes, allowing a better utilisation of resources and increasing the improvement efficiency (Poehlman, ). Recently our research group has generated a sexual synthetic population of P. notatum , attempting to transfer the genetic variability from the apomictic to the sexual tetraploid germplasm (Zilli et al, ). This population is expected to be the base gene pool for breeding of tetraploid P. notatum .…”

Section: Discussioncontrasting

confidence: 71%

“…The second group was formed by 10 NATG collected along the natural distribution range of the species (Figure ). Finally, the third group consisted of 306 genotypes belonging to a SSTP (Zilli et al, ). This population was created by intercrossing 29 sexual F 1 hybrids, which were generated by crossing the sexual germplasm named ESTG with the apomictic ecotypes identified as NATG.…”

Section: Methodsmentioning

confidence: 99%

“…With the hypothesis that the natural diversity can be transferred from the apomictic germplasm of P. notatum to a synthetic sexual population, the following three‐step procedure was used: (a) crosses were made between three ESTG and 10 natural apomictic tetraploid genotypes (NATG; Zilli et al, ); (b) sexual hybrids were identified by molecular markers linked to apospory and (c) a sexual synthetic population was created by intercrossing 29 sexual F 1 hybrids (Zilli et al, ). This population could be of great utility for breeding the species; however, there is insufficient knowledge about the level of genetic variability in comparison with the apomictic wild and sexual experimental germplasms.…”

Section: Introductionmentioning

confidence: 99%

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Transference of natural diversity from the apomictic germplasm of Paspalum notatum to a sexual synthetic population

Zilli

Acuña

Schulz

et al. 2019

Annals of Applied Biology

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Genetic improvement in apomictic forage species has been restricted because of the absence of genetic variability in sexual germplasm with the same ploidy level. Following a new breeding scheme, a sexual synthetic tetraploid population (SSTP) of Paspalum notatum has been generated. The objectives of this work were: (a) to evaluate the genetic variability in SSTP by means of molecular markers, morphologic and agronomic traits, and seed fertility and quality traits and (b) to assess the transference of genetic variability from the apomictic germplasm to the sexual one. Molecular markers revealed a twofold higher level of variability in the SSTP in comparison with the sexual germplasm utilised for its generation, and similar levels with the apomictic ones; moreover, markers showed that most of the variability was inherited from the apomictic germplasm. Morphologic and agronomic traits and seed fertility and quality traits showed high levels of variation in the three groups of genotypes indicating that the new breeding scheme was effective in transferring variability from the apomictic germplasm to the SSTP. This new population will be useful in breeding of P. notatum, and the breeding scheme used for its generation may be used in other apomictic species. K E Y W O R D Sfertility, genetic improvement, genetic variability, microsatellite markers, sexuality

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Section: Discussioncontrasting

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Transference of natural diversity from the apomictic germplasm of Paspalum notatum to a sexual synthetic population

Zilli

Acuña

Schulz

et al. 2019

Annals of Applied Biology

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“…Furthermore, considering the ecological amplitude found among species, and the reproductive, morphological, and genetic variability present in the group (Zuloaga and Morrone 2005), Paspalum is of scientific interest for a variety of studies, including topics on evolution, like the origin and distribution of new apomicts (e.g. Hojsgaard 2018) and applied agronomy, like breeding of native forages (Marcón et al 2018;Zilli et al 2018Zilli et al , 2019. Many Paspalum species are characterized by the existence of conspecific cytotypes, i.e.…”

Section: Introductionmentioning

confidence: 99%

New records and range expansion for Paspalum procurrens and P. volcanense in northwestern Argentina and southeastern Bolivia

Glücksberg¹,

Martínez²,

Honfi³

et al. 2019

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Paspalum procurrens Quarin and P. volcanense Zuloaga, Morrone & Denham are two rare species of South American grasses inhabiting geographically restricted areas which are exposed to ecological degradation due to landscape transformation and biodiversity losses. We present new records for these species, from the provinces of Jujuy, Salta, Tucumán and Catamarca (Argentina) and departments of Tarija, Chuquisaca, and Santa Cruz (Bolivia). New geographic distribution maps and the biological relevance of these findings are discussed pinpointing the need for imperative and assiduous botanical explorations in biodiversity hotspots sensible to the impact of human activities. Ploidy levels in these two species are studied.

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“…Most available methods rely on reproductive tissues or observation of progeny. Embryo sac observation is the most popular method among plant breeders (Weiler et al, 2017; Zilli et al, 2018), since it is inexpensive and reliable. Flow cytometric seed analysis is also effectively used for some species (Novo et al, 2017).…”

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confidence: 99%

Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts, and Impact of Apomixis on Hybrid Performance

et al. 2019

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Apomictic grasses predominate among tropical forages, and specific breeding techniques are needed for their genetic improvement. The objectives of this study were (i) to generate hybrids by crossing tetraploid sexual and apomictic Paspalum simplex Morong genotypes, (ii) to develop a technique based on molecular markers for early identification of apomictic hybrids, and (iii) to determine the relationship between mode of reproduction and performance of hybrids. Crosses were made between two induced sexual and seven apomictic tetraploid plants. Identification of apomictic hybrids during seedling stage was performed using a marker linked to apomixis, and by flow cytometric seed analysis. Hybrids were evaluated under field conditions for plant diameter and height, initial growth, and seasonal regrowth during two consecutive growing seasons. Sexual tetraploid plants used as female parents behaved as allogamous, since 95% of the progeny had male‐specific markers. The ratio between apomictic and sexual hybrids differed from 1:8.7 to 1:0.6 among crosses, with a mean of 1:2.4. There was a 96% coincidence between the use of the apomixis‐linked marker and flow cytometric seed analysis. A technique based on the use of early DNA isolation and the amplification of a molecular marker linked to apomixis was developed. We found no overall difference between apomictic and sexual hybrids for the evaluated traits, except for fall regrowth in the first year, for which apomictic hybrids were superior. Generation of large tetraploid hybrid progenies is possible in P. simplex. Segregation for mode of reproduction depends on parents involved. Agronomic performance is not related to reproductive mode among tetraploid hybrids.

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Widening the Gene Pool of Sexual Tetraploid Bahiagrass: Generation and Reproductive Characterization of a Sexual Synthetic Tetraploid Population

Cited by 24 publications

References 60 publications

Transference of natural diversity from the apomictic germplasm of Paspalum notatum to a sexual synthetic population

Transference of natural diversity from the apomictic germplasm of Paspalum notatum to a sexual synthetic population

New records and range expansion for Paspalum procurrens and P. volcanense in northwestern Argentina and southeastern Bolivia

Breeding Tetraploid Paspalum simplex: Hybridization, Early Identification of Apomicts, and Impact of Apomixis on Hybrid Performance

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