Widespread effect of N-acetyl-d-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis

Inokuma, Kentaro; Matsuda, Mami; Sasaki, Daisuke; Hasunuma, Tomohisa; Kondo, Akihiko

doi:10.1186/s12934-018-0998-4

Cited by 14 publications

(11 citation statements)

References 33 publications

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“…Total RNA was extracted from the cells harvested at 48 h fermentation using the NucreoSpin RNA (Macherey–Nagel, Düren, Germany) according to the manufacturer’s instructions. The expression levels of ARO4 and ARO7 genes were quantified by real-time PCR as previously described ( Inokuma et al, 2018 ). All the primers used for qRT-PCR are listed in Supplementary Table S1 .…”

Section: Methodsmentioning

confidence: 99%

Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

Kobayashi

Inokuma

Matsuda

et al. 2021

Metabolic Engineering Communications

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Resveratrol is a plant-derived aromatic compound with a wide range of beneficial properties including antioxidant and anti-aging effects. The resveratrol currently available on the market is predominantly extracted from certain plants such as grape and the Japanese knotweed Polygonum cuspidatum . Due to the unstable harvest of these plants and the low resveratrol purity obtained, it is necessary to develop a stable production process of high-purity resveratrol from inexpensive feedstocks. Here, we attempted to produce resveratrol from a wide range of sugars as carbon sources by a using the genetically-engineered yeast Scheffersomyces stipitis (formerly known as Pichia stipitis ), which possesses a broad sugar utilization capacity. First, we constructed the resveratrol producing strain by introducing genes coding the essential enzymes for resveratrol biosynthesis [tyrosine ammonia-lyase 1 derived from Herpetosiphon aurantiacus ( HaTAL1 ), 4-coumarate: CoA ligase 2 derived from Arabidopsis thaliana ( At4CL2 ), and stilbene synthase 1 derived from Vitis vinifera ( VvVST1 )]. Subsequently, a feedback-insensitive allele of chorismate mutase was overexpressed in the constructed strain to improve resveratrol production. The constructed strain successfully produced resveratrol from a broad range of biomass-derived sugars [glucose, fructose, xylose, N -acetyl glucosamine (GlcNAc), galactose, cellobiose, maltose, and sucrose] in shake flask cultivation. Significant resveratrol titers were detected in cellobiose and sucrose fermentation (529.8 and 668.6 mg/L after 120 h fermentation, respectively), twice above the amount obtained with glucose (237.6 mg/L). Metabolomic analysis revealed an altered profile of the metabolites involved in the glycolysis and shikimate pathways, and also of cofactors and metabolites of energy metabolisms, depending on the substrate used. The levels of resveratrol precursors such as L-tyrosine increased in cellobiose and sucrose-grown cells. The results indicate that S. stipitis is an attractive microbial platform for resveratrol production from broad types of biomass-derived sugars and the selection of suitable substrates is crucial for improving resveratrol productivity of this yeast.

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Section: Methodsmentioning

confidence: 99%

Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

Kobayashi

Inokuma

Matsuda

et al. 2021

Metabolic Engineering Communications

Self Cite

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“…It was reported that Scheffersomyces stipitis uses N ‐acetyl‐ d ‐glucosamine (GlcNAc) as its sole carbon source, but the GlcNAc metabolic pathway in S. stipitis is still poorly understood. In order to investigate the metabolic responses to GlcNAc in S. stipitis , Inokuma et al conducted both cationic and anionic analyses on a CE‐MS system with sheath‐liquid interface [38]. The harvested yeast cells were first subjected to anaerobic fermentation for 24 h prior to metabolite extraction using a boiling ethanol method at 95°C for 5 min.…”

Section: Applicationsmentioning

confidence: 99%

CE‐MS for metabolomics: Developments and applications in the period 2018–2020

Zhang

Ramautar

2020

Electrophoresis

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Capillary electrophoresis‐mass spectrometry (CE‐MS) is now a mature analytical technique in metabolomics, notably for the efficient profiling of polar and charged metabolites. Over the past few years, (further) progress has been made in the design of improved interfacing techniques for coupling CE to MS; also, in the development of CE‐MS approaches for profiling metabolites in volume‐restricted samples, and in strategies that further enhance the metabolic coverage. In this article, which is a follow‐up of a previous review article covering the years 2016–2018 (Electrophoresis 2019, 40, 165–179), the main (technological) developments in CE‐MS methods and strategies for metabolomics are discussed covering the literature from July 2018 to June 2020. Representative examples highlight the utility of CE‐MS in the fields of biomedical, clinical, microbial, plant and food metabolomics. A complete overview of recent CE‐MS‐based metabolomics studies is given in a table, which provides information on sample type and pretreatment, capillary coatings, and MS detection mode. Finally, some general conclusions and perspectives are given.

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“…Organic acids were analysed using high-performance liquid chromatography (Shimadzu LC-10AD) as described previously (Mohd Yusoff et al 2012). Ammonia concentration in WSS supernatant was measured using ammonia assay kit (Wako, Japan) (Inokuma et al 2018). Each assay was conducted at least in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Effect of Aso limonite on anaerobic digestion of waste sewage sludge

2020

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The effect of Aso volcanic limonite was explored in anaerobic digestion using waste sewage sludge (WSS). In this study, methane and hydrogen sulfide were remarkably inhibited when Aso limonite was mixed with WSS as well as a significant reduction of ammonia. Although pH was lowered after adding Aso limonite, methane was still inhibited in neutralized pH condition at 7.0. Hydrolysis stage was not influenced by Aso limonite as supported by the result that a high protease activity was still detected in the presence of the material. However, acidogenesis stage was affected by Aso limonite as indicated by the different productions of organic acids. Acetic acid, was accumulated in the presence of Aso limonite due to the inhibition of methane production, except in the highest concentration of Aso limonite which the production of acetate may be inhibited. Besides, the production of propionate and butyrate reduced in accordance to the increased concentration of Aso limonite. In addition, Archaeal activity (methanogens) in WSS with Aso limonite was low in agreement with the low methane production. Thus, these results indicate that Aso limonite influences the acidogenesis and methanogenesis processes, by which the productions of methane and ammonia were inhibited. On the other hand, in the contactless of Aso limonite during the anaerobic digestion of WSS (Aso limonite was placed in the area of headspace in the vial), Aso limonite had the adsorptive ability for hydrogen sulfide from WSS, but not for methane. This contactless system of Aso limonite may be a practical means to remove hydrogen sulfide without inhibiting methane production as an important bioenergy source. Publisher's NoteSpringer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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Widespread effect of N-acetyl-d-glucosamine assimilation on the metabolisms of amino acids, purines, and pyrimidines in Scheffersomyces stipitis

Cited by 14 publications

References 33 publications

Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

Resveratrol production from several types of saccharide sources by a recombinant Scheffersomyces stipitis strain

CE‐MS for metabolomics: Developments and applications in the period 2018–2020

Effect of Aso limonite on anaerobic digestion of waste sewage sludge

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