Wie sind Reihenversuche für biologische Auswertungen am zweckmäßigsten anzuordnen?

Behrens, Behrend; Kärber, G.

doi:10.1007/bf02023142

Cited by 144 publications

(33 citation statements)

References 5 publications

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“…MiceLei-Dab 7 was administered to 25-g C57/BL6 mice via the intracerebroventricular route, and the LD 50 was determined (33). Groups of four mice per dose were injected with 5 l of the peptide solution containing 0.1% (w/v) bovine serum albumin and 0.9% (w/v) sodium chloride.…”

Section: Selective In Vivo Blockade Of Skca2 Channels By Lei-dab 7 Inmentioning

confidence: 99%

Design and Characterization of a Highly Selective Peptide Inhibitor of the Small Conductance Calcium-activated K+Channel, SkCa2

Shakkottai¹,

Regaya²,

Wulff³

et al. 2001

Journal of Biological Chemistry

111

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Apamin-sensitive small conductance calcium-activated potassium channels (SKCa1-3) mediate the slow afterhyperpolarization in neurons, but the molecular identity of the channel has not been defined because of the lack of specific inhibitors. Here we describe the structure-based design of a selective inhibitor of SKCa2. Leiurotoxin I (Lei) and PO5, peptide toxins that share the RXCQ motif, potently blocked human SKCa2 and SKCa3 but not SKCa1, whereas maurotoxin, Pi1, Ts, and PO1 were ineffective. Lei blocked these channels more potently than PO5 because of the presence of

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Section: Selective In Vivo Blockade Of Skca2 Channels By Lei-dab 7 Inmentioning

confidence: 99%

Design and Characterization of a Highly Selective Peptide Inhibitor of the Small Conductance Calcium-activated K+Channel, SkCa2

Shakkottai¹,

Regaya²,

Wulff³

et al. 2001

Journal of Biological Chemistry

111

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“…Qualitative and quantitative toxicity measurements were carried out, according to Behrens and Karber [10], using C3H/He mice of 20 +-3 g (C.S.E.A.L. -C.N.R.S., Orl6ans-la-Source, France) as previously described [5,7].…”

Section: Purification Of the Toxinsmentioning

confidence: 99%

Purification of animal neurotoxins: Isolation and characterization of three neurotoxins from the venom of Naja nigricollis mossambica Peters

Rochat¹,

Gregoire²,

Martin-Moutôt³

et al. 1974

FEBS Letters

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“…The GFM-1 strain (9) of APV was used as a positive control (Table 1). It was used at a titer of 10 5.8 TCID 50 /ml as measured by the Behrens-Kärber method (3).…”

Section: Methodsmentioning

confidence: 99%

Duplex Shuttle PCR for Differential Diagnosis of Budgerigar Fledgling Disease and Psittacine Beak and Feather Disease

Ogawa

Yamaguchi

Fukushi

2005

Microbiology and Immunology

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Two common viral diseases in psittacine birds including budgerigar fledgling disease (BFD), generally called avian polyomavirus (APV) infection, and psittacine beak and feather disease (PBFD) have similar clinical manifestations characterized by feather disorders. A duplex shuttle PCR was developed for detection of APV and PBFD virus (PBFDV). Two pairs of oligonucleotide primers were designed to amplify a 298‐bp fragment of the t/T antigen region of APV genome and a 495‐bp fragment of the capsid protein region encoded by open reading frame (ORF) C1 of PBFDV genome, respectively. In the present study, APV and PBFDV were detected simultaneously in one tube by duplex shuttle PCR using these two pairs of primers. The detection limits were 2 viral copies of APV and 3 viral copies of PBFDV. In the clinical application, we detected 16 APV‐positive, 15 PBFDV‐positive, and 3 mixed infected samples in 39 samples examined. Sequences of the amplified products were read. The t/T antigen region was conserved in the APV‐positive samples as expected. ORF C1 of PBFDV genome showed diversity. Phylogenic analysis indicated that PBFDV ORF C1 consisted of 6 clusters which were related to subfamilies of psittacine birds. Our duplex shuttle PCR could be a useful method for differential diagnosis and molecular epidemiology of BFD and PBFD.

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Wie sind Reihenversuche für biologische Auswertungen am zweckmäßigsten anzuordnen?

Cited by 144 publications

References 5 publications

Design and Characterization of a Highly Selective Peptide Inhibitor of the Small Conductance Calcium-activated K+Channel, SkCa2

Design and Characterization of a Highly Selective Peptide Inhibitor of the Small Conductance Calcium-activated K+Channel, SkCa2

Purification of animal neurotoxins: Isolation and characterization of three neurotoxins from the venom of Naja nigricollis mossambica Peters

Duplex Shuttle PCR for Differential Diagnosis of Budgerigar Fledgling Disease and Psittacine Beak and Feather Disease

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