Wild blueberry (V. angustifolium) improves TNFα-induced cell barrier permeability through claudin-1 and oxidative stress modulation in Caco-2 cells

Abstract

Dietary doses of wild blueberry, rich in bioactive components such as polyphenols, may contribute to support the integrity of the intestinal barrier by preserving claudin-1 expression and reducing markers of oxidative stress.

Red raspberry (Rubus idaeus) preserves intestinal barrier integrity and reduces oxidative stress in Caco-2 cells exposed to a proinflammatory stimulus

“…Our findings align with those of other studies examining PP from different berries, such as blueberries, which have demonstrated beneficial effects on IB integrity using the same Caco-2 cellular model and assay. 36–40 We have recently reported the capacity of a wild blueberry powder to preserve IB integrity and permeability as documented by an increase in TEER and a decrease in FITC particularly correlated to a reduction of the levels of 8-OHdG and the preservation of the integral membrane protein claudin-1. 39 Although the different powders showed similar results, the RB powder demonstrated the capacity to counteract the intestinal barrier disruption also at the lower concentration.…”

Dietary Polyphenols, Plant Metabolites, and Allergic Disorders: A Comprehensive Review

“…Improvement in the intestinal barrier function and maintenance of the integrity of the intestinal cell monolayer; decreased gut permeability, higher transepithelial electrical resistance (TEER), elevated expression of claudin-1 [204] Ferulic acid caffeic acid apigenin luteolin Food allergy 1-3 g/kg/day of olive oil BALB/c mice (in vivo)…”

Dietary Polyphenols—Natural Bioactive Compounds with Potential for Preventing and Treating Some Allergic Conditions

“…Indeed, evidence from in vitro and animal studies has indicated that dietary polyphenols can alleviate intestinal barrier dysfunction and reduce intestinal permeability through different mechanisms including the upregulation of the intestinal tight junction (TJs) protein expression, increase in trans epithelial electrical resistance (TEER) across a cellular monolayer, reduction in oxidative stress and inhibition of signaling pathways such as nuclear factor kappa β (NF-κβ) and mitogenactivated protein kinases (MAPK) involved in the inflammation process [182,[228][229][230]. The main evidence on the beneficial effect on intestinal barrier function and integrity and thus alleviation of food allergy symptoms are available for quercetin, luteolin, naringenin, kaempferol, curcumin, green and black tea flavonoids, grape seed proanthocyanidin, wild blubbery anthocyanins, and chlorogenic acids, tested in doses ranging from physiological (i.e., epicatechin) to pharmacological concentrations (i.e., berberine) [188,189,225,[230][231][232][233][234]. Recently, the capacity of polyphenols to prevent food allergy by regulating intestinal immu-nity and improving intestinal barrier function has been well illustrated by studies carried out with olive oil, one of the main components of the Mediterranean diet, containing a high concentration of polyphenols such as phenolic acids (ferulic and caffeic), lignans and flavones (apigenin, luteolin) [68].…”