Wild Ecotopes and Food Habits of Triatoma longipennis Infected by Trypanosoma cruzi Lineages I and II in Mexico

Bosseno, Marie-France; Barnabé, Christian; Sierra, Maria Jesus Ramirez; Kengne, Pierre; Guerrero, Sandra; Lozano, Felipe Domı́nguez; Ezequiel, Kasten; Gastélum, Magallón; Brenière, Simone Frédérique

doi:10.4269/ajtmh.2009.80.988

Cited by 29 publications

(21 citation statements)

References 16 publications

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“…In Mexico, most T. cruzi strains have been genetically determined to belong to TcI (Bosseno et al 2002, Leó n-Pérez et al 2007, Gó mez-Hernández et al 2011 and TcII-TcVI strains have been reported only in a recent serological study, in wild mammals and the feces of vectors (Ló pez-Olmos et al 1998, Bosseno et al 2009, Risso et al 2011. In this work, we confirmed that the Mexican T. cruzi strains isolated from humans belonged to TcI, as reported in Guatemala, Colombia, and Venezuela (Añ ez et al 2004, Ruíz-Sánchez et al 2005, Falla et al 2009).…”

Section: Discussionsupporting

confidence: 79%

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Martı́nez

Nogueda

Martínez‐Hernández

et al. 2013

Vector-Borne and Zoonotic Diseases

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Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and it affects as many as 10 million people in North and South America, where it represents a major public health problem. T. cruzi is a parasite with high genetic diversity, and it has been grouped into 6 discrete typing units (DTUs), designated as T. cruzi I (TcI) to T. cruzi VI (TcVI). Mexican isolates from humans and from vector insects have been primarily found to be TcI, and these isolates are likely to be the strains that cause the clinical manifestations observed in Mexico. However, genetic characterization and drug susceptibility assays are limited in Mexican TcI strains. In this work, 24 Mexican T. cruzi strains, obtained primarily from humans, were studied with 7 locus microsatellites and mini-exon gene by PCR. Also, drug susceptibility was evaluated by growth and mobility assays. All of the human strains belonged to TcI, and they could be further grouped through microsatellite analysis into 2 subgroups (microsatellite genotypes 1 and 2), which were not related to the host clinical status or biological origin of the strain. Two strains, both from wild mammals, belonged to the TcII-TcVI groups; these strains and the CL Brener strain constituted microsatellite genotype 3. The number of alleles in each locus was lower than reported for South American strains, and a departure from the Hardy-Weinberg equilibrium was observed. The susceptibility of these strains to nifurtimox and benznidazole was heterogeneous. T. cruzi strains characterized as microsatellite genotypes 2 and 3 were significantly more susceptible to benznidazole than strains of microsatellite genotype 1. Only 1 Mexican strain resistant to both drugs was found in this study.

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Section: Discussionsupporting

confidence: 79%

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Martı́nez

Nogueda

Martínez‐Hernández

et al. 2013

Vector-Borne and Zoonotic Diseases

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“…This relationship between genotype and T. cruzi transmission cycle cannot be applied to countries in Central America or Mexico because genotype TcI is the main group isolated from domestic, peridomestic, and sylvatic transmission cycles in these regions [9][10][11] . In the present study, different molecular biology techniques demonstrated that the isolates studied belonged to the TcI group.…”

Section: Discussionmentioning

confidence: 99%

“…In Mexico, genetic characterization by miniexon analysis of isolates obtained from triatomines, domestic and wild reservoirs, and human cases with cardiac manifestations revealed the presence of T. cruzi group I [9][10][11] . However, there have been few reports of T. cruzi II in this country 11 .…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Gómez-Hernández

Rezende-Oliveira

Nascentes

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. Methods: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD -(random amplified polymorphic DNA). Results: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10 6 to 10 7 parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. Conclusions: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Keywords: Trypanosoma cruzi. Mexico. Biological characterization. Genetic characterization. RESUMOIntrodução: Durante muito tempo, foi questionada a importância da doença de Chagas no México onde muitos a consideravam um padecimento exótico. Considerando a grande diversidade genética existente, entre os isolados de Trypanosoma cruzi, a importância da caracterização biológica desses e o escasso número de informações sobre os aspectos clínicos e biológicos da doença de Chagas no México, o objetivo deste trabalho foi realizar a caracterização biológica e molecular de isolados de Trypanosoma cruzi originários de diferentes áreas endêmicas deste país, principalmente do Estado de Jalisco. Métodos: Oito cepas mexicanas de Trypanosoma cruzi foram caracterizadas biologicamente e geneticamente (PCR específica para Trypanosoma cruzi, PCR-multiplex, amplificação do espaço não transcrito dos genes do mini-exon, amplificação das regiões polimórficas do gene do mini-exon, classificação pela amplificação de regiões intergênicas dos genes do spliced leader, RAPD -random amplified polymorphic DNA). Resultados: Foram observados dois tipos de parasitemia: patente com picos máximos de parasitemia entre 4,6x10 6 e 10 7 parasitas/mL e subpatente. Além disso, todos os isolados foram capazes de infectar 100% dos animais. Observou-se tropismo predominante pelo músculo estriado (cardíaco e esquelético). As técnicas de PCR do gene do mini-éxon classificaram as oito cepas como TcI e a técnica de RAPD mostrou variaçã...

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“…Nevertheless, the role of others mammals in sylvatic or peridomestic cycles of transmission of T. cruzi is not well known. For example, in the occidental part of Mexico, wild triatomine populations were highly parasitized with T. cruzi and the principal feeding resource were Dasypus nomencinctus and Procyon lotor , suggesting that these mammals are key reservoirs in that region [13]. On the other hand, in Brazil and USA, different species of the Procyonidae family have also been reported to be infected with T. cruzi [14–17].…”

Section: Introductionmentioning

confidence: 99%

Follow up of natural infection with Trypanosoma cruzi in two mammals species, Nasua narica and Procyon lotor (Carnivora: Procyonidae): evidence of infection control?

Martínez‐Hernández

Rendón-Franco²,

Campillo

et al. 2014

Parasites Vectors

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BackgroundA large variety of mammals act as natural reservoirs of Trypanosoma cruzi (the causal agent of Chagas disease) across the American continent. Related issues are infection and parasite burden in these reservoirs, and whether they are able to control T. cruzi infections. These parameters can indicate the real role of mammals as T. cruzi reservoirs and transmitters. Here, two species of mammals, white-nosed coati (Nasua narica) and raccoon (Procyon lotor), were examined for to determine: a) T. cruzi presence, and; b) their ability to control T. cruzi infection.MethodsMultiple capture-recaptures of both species were carried out in semi-wild conditions in Villahermosa, Tabasco, Mexico, for 5 years. Two samplings per year (summer and winter) took place. Prevalence and pattern of T. cruzi infection were determined by PCR from both mammals’ blood samples.ResultsRaccoon samples had a higher relative infection values (26.6%) compared to those of white-nosed coati (9.05%), being this difference significant in summer 2012 (P < 0.00001), summer (P = 0.03) and winter 2013 (P = 0.02). Capture and recapture data indicated three infection dynamics: 1) negative–positive-negative infection; 2) positive–negative-positive infection; and 3) positive at all sampling times.ConclusionsThese results indicate that both coati and raccoons may be able to control T. cruzi infection. Thus, the role as efficient reservoirs could be questioned (at least for those times when mammals are able to tolerate the infection). However, while infected, they may also be able to approach human dwellings and play a role important in linking sylvatic and domestic cycles.

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Wild Ecotopes and Food Habits of Triatoma longipennis Infected by Trypanosoma cruzi Lineages I and II in Mexico

Cited by 29 publications

References 16 publications

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Microsatellite and Mini-Exon Analysis of Mexican Human DTU ITrypanosoma cruziStrains and Their Susceptibility to Nifurtimox and Benznidazole

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Follow up of natural infection with Trypanosoma cruzi in two mammals species, Nasua narica and Procyon lotor (Carnivora: Procyonidae): evidence of infection control?

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