Wild mushrooms showed analgesic and cytotoxic properties along with phytoconstituent's binding affinity to COX-1, COX-2 and cytochrome P450 2C9

Abstract

This study was designed to evaluate the cytotoxic and analgesic potential of methanol extracts of five wild mushrooms available in the University of Chittagong, Bangladesh. The acetic acid-induced writhing method was used for the analgesic activity, while cytotoxicity was tested using brine shrimp lethality bioassay. In silico molecular docking and ADME/T study have been employed by using Schrodinger v11.1, BIOVIA Discovery Studio 2020 and online tool (AdmeSAR) respectively. The methanol extracts of Daldinia concentrica , Trametes lactinea , Fomitopsis cajanderi and Daedaleopsis confragosa exhibited a significant ( p < 0.001) decrease in the number of writhing when compared to the control group. Except for Lentinus squarrosulus at 200 mg/kg body weight, the remaining mushroom extracts showed equal to or above 50 % inhibition of writhing. Daldinia concentrica showed the lowest LC 50 values with 0.63 μg/mL, while Daedaleopsis confragosa showed the highest LC 50 values of 2.33 μg/mL, indicating decisive cytotoxic action all mushrooms extracts. Considering the secondary metabolites, daldinan A and fomlactone A were found the most promising myco-compounds in analgesic and cytotoxic molecular docking studies. Besides, all the selected metabolites meet the rule of Lipinski's drug-likeliness. These results concluded that each mushroom extracts except Lentinus squarrosulus possess a potential analgesic. All the mushroom extracts embrace a promising cytotoxic activity that may guide the progress of a new drug.

Comparative Study on Biological Activities and Chemical Profiling of Mushroom (Pleurotus pulmonarius (Fr.) Quel.) Cultured on Lignocellulosic Agro Wastes

“…At the same time, the presence of fatty acids, proteins, phenolic compounds, steroids, and terpenoids may be responsible for the high antiproliferative and antioxidant activities of the various mushroom extracts. [31,[38][39][40][41][42][43][44][45][46][47] The higher antioxidant activities of the aqueous extracts also suggest the existence of remarkable polar and active constituents in the mushroom extracts as established by the LC/MS data and in agreement with the literature. [29,30] Admittedly, the nutritional composition of the various mushrooms and their secondary metabolites were not evaluated in the present study.…”

“…The low activity of P. pulmonarius extracts obtained in this study may be due to the decrease in the polysaccharides concentration, which, in turn, may be a result of the internal β‐glucanase activity. At the same time, the presence of fatty acids, proteins, phenolic compounds, steroids, and terpenoids may be responsible for the high antiproliferative and antioxidant activities of the various mushroom extracts [31,38–47] . The higher antioxidant activities of the aqueous extracts also suggest the existence of remarkable polar and active constituents in the mushroom extracts as established by the LC/MS data and in agreement with the literature [29,30] .…”

Modulating opto‐electronic and magnetic responses of Mo‐ and Zn‐adsorbed LiNbO3 (1 1 1) surface for advanced energy harvesting applications: A comprehensive study

“…Our energy gap results, the energy gap of 1.04 eV would be the most suitable for photovoltaic applications, followed by 0.70, 1.02 eV, and lastly, 0.30 eV. The band gap for (LiNbO 3 ) crystal is 3.54 eV [69]. In our calculations, the band gap of Mo, Zn/LiNbO 3 (1 1 1) material was found to vary between 0.30 and 1.04 eV.…”

Neuropharmacological and antiproliferative activity of Tetrastigma leucostaphyllum (Dennst.) Alston: Evidence from in-vivo, in-vitro and in-silico approaches

“…This is because CYP2C9 is involved in the way that many substances, like some medicines and toxins, are broken down in the body. Therefore, considering these facts, potassium channel KCSA-FAB (PDB: 4UUJ) ( Lenaeus et al, 2014 ), human corticotrophin-releasing factor (PDB: 4K5Y) ( Hollenstein et al, 2013 ), human serotonin transporter (PDB: 5I6X) ( Coleman et al, 2016 ), human serotonin transporter (PBD: 6VRH) ( Coleman et al, 2020 ), and human cytochrome P450 CYP2C9 (PDB: 1OG5) ( Moazzem Hossen et al, 2021 ) were obtained from the RCSB Protein Data Bank (PDB). These elements have been discovered in humans like; Homo sapiens, Mus musculus, and Escherichia virus T4.…”