Wild peanut Arachis duranensis are nodulated by diverse and novel Bradyrhizobium species in acid soils

Chen, Jing Yu; Gu, Jun; Wang, En Tao; Xian, Xing; Kang, Shi Tong; Huang, Ling; Cao, Xue; Li, Liang Bing; Wu, Yan

doi:10.1016/j.syapm.2014.05.004

Cited by 22 publications

(8 citation statements)

References 43 publications

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“…These data confirmed the high level symbiotic promiscuity of this legume. The fact that all the selected test isolates (both Bradyrhizobium and Rhizobium ) could, in fulfilment of Koch’s postulates, form effective root nodules on groundnut (the homologous host) is in contrast to the results of Chen et al [7] who found that only species of Bradyrhizobium nodulated groundnut, whereas Wong et al [56] reported that groundnut nodules formed by fast-growing rhizobia were ineffective.…”

Section: Discussionmentioning

confidence: 60%

“…To date, eight defined Bradyrhizobium species (namely, B. japonicum , B. elkanii , B. lablabi , B. yuanmingense , B. iriomotense , B. guangxiense , B. guangdongense and Bradyrhizobium arachidis ) and an unidentified Bradyrhizobium sp. have been reported to be capable of nodulating groundnut [7], [26], [31], [33], [39], [48], [49], [51], [59]. In Group I, isolates TUTAHSA67, TUTAHSA51 and TUTAHSA75 were closely related to B. guangdongense with 95.2–95.4% sequence identity.…”

Section: Discussionmentioning

confidence: 99%

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Phylogenetically diverse group of native bacterial symbionts isolated from root nodules of groundnut ( Arachis hypogaea L.) in South Africa

Jaiswal

Msimbira

Dakora

2017

Systematic and Applied Microbiology

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Groundnut is an economically important N2-fixing legume that can contribute about 100–190 kg N ha−1 to cropping systems. In this study, groundnut-nodulating native rhizobia in South African soils were isolated from root nodules. Genetic analysis of isolates was done using restriction fragment length polymorphism (RFLP)-PCR of the intergenic spacer (IGS) region of 16S-23S rDNA. A total of 26 IGS types were detected with band sizes ranging from 471 to 1415 bp. The rhizobial isolates were grouped into five main clusters with Jaccard's similarity coefficient of 0.00–1.00, and 35 restriction types in a UPGMA dendrogram. Partial sequence analysis of the 16S rDNA, IGS of 16S rDNA-23S rDNA, atpD, gyrB, gltA, glnII and symbiotic nifH and nodC genes obtained for representative isolates of each RFLP-cluster showed that these native groundnut-nodulating rhizobia were phylogenetically diverse, thus confirming the extent of promiscuity of this legume. Concatenated gene sequence analysis showed that most isolates did not align with known type strains, and may represent new species from South Africa. This underscored the high genetic variability associated with groundnut Rhizobium and Bradyrhizobium in South African soils, and the possible presence of a reservoir of novel groundnut-nodulating Bradyrhizobium and Rhizobium in the country.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 99%

Phylogenetically diverse group of native bacterial symbionts isolated from root nodules of groundnut ( Arachis hypogaea L.) in South Africa

Jaiswal

Msimbira

Dakora

2017

Systematic and Applied Microbiology

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“…The exclusive recovery of nod ‐containing WBAH strains from Arachis confirms this legume to predominantly use Nod ‐ factor signaling to select the cognate Bradyrhizobium and counterselect the rest (Steenkamp et al ., ; Ibáñez and Fabra, ; Wang et al ., ; Chen et al ., ). However, in absence of WBAH, under controlled conditions, the nod‐ deficient WBOS strains could consistently induce few nodules in Arachis , indicating that the absence of Nod‐factor does not limit the establishment of nodulation and intracellularisation of symbionts in Arachis .…”

Section: Discussionmentioning

confidence: 97%

Segregation of nod‐containing and nod‐deficient bradyrhizobia as endosymbionts of Arachis hypogaea and as endophytes of Oryza sativa in intercropped fields of Bengal Basin, India

Guha

Sarkar

Ganguly

et al. 2016

Environmental Microbiology

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Bradyrhizobial invasion in dalbergoid legumes like Arachis hypogaea and endophytic bacterial invasions in non-legumes like Oryza sativa occur through epidermal cracks. Here, we show that there is no overlap between the bradyrhizobial consortia that endosymbiotically and endophytically colonise these plants. To minimise contrast due to phylogeographic isolation, strains were collected from Arachis/Oryza intercropped fields and a total of 17 bradyrhizobia from Arachis (WBAH) and 13 from Oryza (WBOS) were investigated. 16SrRNA and concatenated dnaK-glnII-recA phylogeny clustered the nodABC-positive WBAH and nodABC-deficient WBOS strains in two distinct clades. The in-field segregation is reproducible under controlled conditions which limits the factors that influence their competitive exclusion. While WBAH renodulated Arachis successfully, WBOS nodulated in an inefficient manner. Thus, Arachis, like other Aeschynomene legumes support nod-independent symbiosis that was ineffectual in natural fields. In Oryza, WBOS recolonised endophytically and promoted its growth. WBAH however caused severe chlorosis that was completely overcome when coinfected with WBOS. This explains the exclusive recovery of WBOS in Oryza in natural fields and suggests Nod-factors to have a role in counterselection of WBAH. Finally, canonical soxY1 and thiosulphate oxidation could only be detected in WBOS indicating loss of metabolic traits in WBAH with adaptation of symbiotic lifestyle.

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“…This process possibly involves a complex evolutionary phenomenon including the horizontal transfer of symbiotic genes into rhizobia strains and the specific legume genotypes located in various geographic zones [24]. For instance, the 16S/23S analysis of 35 strains from genus Bradyrhizobium showed very high similarity (95%-100%) and also confirmed that Bradyrhizobium is a major root nodule symbiotic generous for nodule development in groundnut across the world [21,24]. Phylogenetic analysis also revealed the presence of a new subgroup of Bradyrhizobium, forming a symbiotic association with groundnut.…”

Section: Diversity Among Bacterial Strains Associated With Groundnut Rnsmentioning

confidence: 80%

“…It is worth mentioning that Bradyrhizobium has a basal position amongst all nitrogen-fixing rhizobia, [18] and is reported to be involved in endophytic or symbiotic associations with plant roots [19]. Groundnut forms effective nodules with rhizobia belonging to genus Bradyrhizobium [20,21]. A high level of species diversity and fixation efficiency of symbionts was reported from different geographical regions by morpho-physiological and molecular methods [22].…”

Section: Diversity Among Bacterial Strains Associated With Groundnut Rnsmentioning

confidence: 99%

Molecular Basis of Root Nodule Symbiosis between Bradyrhizobium and ‘Crack-Entry’ Legume Groundnut (Arachis hypogaea L.)

Sharma

Bhattacharyya

Kumar

et al. 2020

Plants

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Nitrogen is one of the essential plant nutrients and a major factor limiting crop productivity. To meet the requirements of sustainable agriculture, there is a need to maximize biological nitrogen fixation in different crop species. Legumes are able to establish root nodule symbiosis (RNS) with nitrogen-fixing soil bacteria which are collectively called rhizobia. This mutualistic association is highly specific, and each rhizobia species/strain interacts with only a specific group of legumes, and vice versa. Nodulation involves multiple phases of interactions ranging from initial bacterial attachment and infection establishment to late nodule development, characterized by a complex molecular signalling between plants and rhizobia. Characteristically, legumes like groundnut display a bacterial invasion strategy popularly known as “crack-entry’’ mechanism, which is reported approximately in 25% of all legumes. This article accommodates critical discussions on the bacterial infection mode, dynamics of nodulation, components of symbiotic signalling pathway, and also the effects of abiotic stresses and phytohormone homeostasis related to the root nodule symbiosis of groundnut and Bradyrhizobium. These parameters can help to understand how groundnut RNS is programmed to recognize and establish symbiotic relationships with rhizobia, adjusting gene expression in response to various regulations. This review further attempts to emphasize the current understanding of advancements regarding RNS research in the groundnut and speculates on prospective improvement possibilities in addition to ways for expanding it to other crops towards achieving sustainable agriculture and overcoming environmental challenges.

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Wild peanut Arachis duranensis are nodulated by diverse and novel Bradyrhizobium species in acid soils

Cited by 22 publications

References 43 publications

Phylogenetically diverse group of native bacterial symbionts isolated from root nodules of groundnut ( Arachis hypogaea L.) in South Africa

Phylogenetically diverse group of native bacterial symbionts isolated from root nodules of groundnut ( Arachis hypogaea L.) in South Africa

Segregation of nod‐containing and nod‐deficient bradyrhizobia as endosymbionts of Arachis hypogaea and as endophytes of Oryza sativa in intercropped fields of Bengal Basin, India

Molecular Basis of Root Nodule Symbiosis between Bradyrhizobium and ‘Crack-Entry’ Legume Groundnut (Arachis hypogaea L.)

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