Wild rice harbors more root endophytic fungi than cultivated rice in the F1 offspring after crossbreeding

Tian, Lei; Wang, Enze; Lin, Xiaochen; Li, Ji; Chang, Jingjing; Chen, Hongping; Wang, Jilin; Chen, Dazhou; Tran, Lam-Son Phan; Tian, Chunjie

doi:10.1186/s12864-021-07587-1

Cited by 10 publications

(7 citation statements)

References 55 publications

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“…The communities of endophytic fungi within the stems were similar to the communities within leaves at all taxonomic levels. Which is also a reasonable agreement with other studies of different plants that contain a higher species richness and diversity in the leaves and stems than in the roots [39]. Since the H. serrata is a perennial plant with a relatively larger surface areas in leaves and stems than in roots.…”

Section: Discussionsupporting

confidence: 92%

Diversity of endophytic fungal community in Huperzia serrata from different ecological areas and their correlation with Hup A content

et al. 2022

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Background Huperzine A (Hup A) has attracted considerable attention as an effective therapeutic candidate drug used to treat Alzheimer’s disease. Whereas, the production of Hup A from wild plants faced a major challenge, which is the wild Huperzia Serrata harbor a low Hup A content, has a long-life cycle, and has a small yield. At present, several reports showed that Hup A is produced by various endophytic fungal strains isolated from H. serrata, thereby providing an alternative method to produce the compound and reduce the consumption of this rare and endangered plant. However, till now, very few comprehensive studies are available on the biological diversity and structural composition of endophytic fungi and the effects of endophytic fungi on the Hup A accumulation in H. serrata. Results In this research, the composition and diversity of fungal communities in H. serrata were deciphered based on high-throughput sequencing technology of fungal internal transcribed spacer regions2 (ITS2). The correlation between endophytic fungal community and Hup A content was also investigated. Results revealed that the richness and the diversity of endophytic fungi in H. serrata was various according to different tissues and different ecological areas. The endophytic fungal communities of H. serrata exhibit species-specific, ecological-specific, and tissue-specific characteristics. There are 6 genera (Ascomycota_unclassified, Cyphellophora, Fungi_unclassified, Sporobolomyces, and Trichomeriaceae_unclassified) were significantly positively correlated with Hup A content in all two areas, whereas, there are 6 genera (Auricularia, Cladophialophora, Cryptococcus, Mortierella, and Mycena) were significantly negatively correlated with Hup A content of in all two areas. Conclusions This study indicated a different composition and diverse endophytic fungal communities in H. serrata from different organs and ecological areas. The current study will provide the realistic basis and theoretical significance for understanding the biological diversity and structural composition of endophytic fungal communities in H. serrata, as well as providing novel insights into the interaction between endophytic fungi and Hup A content.

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Section: Discussionsupporting

confidence: 92%

Diversity of endophytic fungal community in Huperzia serrata from different ecological areas and their correlation with Hup A content

et al. 2022

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“…The communities of endophytic fungi within the stems were similar to the communities within leaves at all taxonomic levels. This is also a reasonable agreement with other studies of different plants that contain a higher species richness as well as diversity in the leaves and stems than in the roots (Ma et al, 2005;Tian et al, 2021).…”

Section: Discussionsupporting

confidence: 93%

Diversity of Endophytic Fungal Community in Huperzia Serrata from Different Ecological Areas and Their Correlation with Hup A Content

Pang

Yin

Zhai

et al. 2022

Preprint

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Hup A has attracted considerable attention as an effective therapeutic candidate drug used for the treatment of Alzheimer’s disease. However, till now, very few comprehensive studies are available on the biological diversity and structural composition of endophytic fungi and the effects of endophytic fungi on the Hup A accumulation in H. serrata. In this research, the composition and diversity of fungal communities were deciphered comparative sequence analysis of the ITS2 region of the fungal rRNA genes based on high-throughput sequencing technology. The correlation between endophytic fungal community and Hup A content was investigated as well. Results revealed that the richness as well as diversity of endophytic fungi was various according to different tissues and different ecological areas. The endophytic fungal communities of H. serrata exhibit specie-specific, ecological-specific and tissue-specific characteristic. This study will provide realistic basis and theoretical significance for understanding the biological diversity and structural composition of endophytic fungal communities in H. serrata, as well as providing novel insights into the interaction between endophytic fungi and Hup A content.

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“…europaea ) had decreased total OTU numbers when compared to plants multiplied under non-aseptic conditions ( 59 ). Systematically compared endophytic microbial community structure of rice in African and Asian cultivars revealed that wild cultivars had more root endophytes when compared to cultivated rice in their first generation following crossbreeding; also network analysis of bacterial and fungal OTUs showed wild species were able to form clusters with higher significant correlations than cultivated rice fungal species ( 60 ). Tissue-specific microbial diversity abundance suggested that the rhizome and roots of both Wt and Tc plants harbor more endophytic bacterial community than leaf tissues, signifying relative and absolute abundances of endophytes are associated with the different parts of the plant.…”

Section: Discussionmentioning

confidence: 99%

Endomicrobiome of in vitro and natural plants deciphering the endophytes-associated secondary metabolite biosynthesis in Picrorhiza kurrooa , a Himalayan medicinal herb

Tamang,

Swarnkar,

Kumar

et al. 2023

Microbiol Spectr

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Picrorhiza kurrooa an endangered high-altitude Himalayan medicinal herb, is used as a potent hepatoprotective due to the presence of various secondary metabolites, with picrosides being the most bioactive. In-vitro propagation is used as a sustainable strategy for its conservation and cultivation. The in-vitro propagation produces P. kurrooa plants (Tc) in mass, but these plants have reduced secondary metabolites (especially picrosides) compared to plants growing in their natural habitats (Wt). Therefore, considering the well-established role of microbes in secondary metabolite biosynthesis, in this study, endomicrobiome of P. kurroa plants (Tc and Wt) was explored. Using high-throughput DNA-sequencing, the endophytic communities associated with leaves, roots, and rhizomes of Wt and Tc plants were characterized. Diversity analysis revealed a loss of diversity during in-vitro propagation, and the abundant phyla were Proteobacteria, Bacteroidetes, Parcubacteria (OD1), Firmicutes, and Verrucomicrobia. Besides, the presence of distinct genera specific to different parts of Wt plants was also revealed. Quantification of secondary metabolites demonstrated the reduced accumulation of picrosides and intermediates of picroside biosynthesis in the Tc plants compared to Wt plants. Host-secondary metabolite production was positively correlated to microbial community abundance, suggesting a dynamic interplay of host-endomicrobiota interaction. Predictive functional analysis revealed the abundance of enzymes of secondary metabolite biosynthesis (especially MVA/MEP and phenylpropanoid/shikimate pathway involved in picrosides biosynthesis) in the associated-endophytic community with predominance in roots and rhizomes of Wt plants. This investigation provides novel insight into the change in the endomicrobiome of Wt and Tc plants and their correlation to the biosynthesis of secondary metabolites, and that needs to be considered for cultivation practices. IMPORTANCE Picrorhiza kurrooa is a major source of picrosides, potent hepatoprotective molecules. Due to the ever-increasing demands, overexploitation has caused an extensive decline in its population in the wild and placed it in the endangered plants' category. At present plant in-vitro systems are widely used for the sustainable generation of P. kurrooa plants, and also for the conservation of other commercially important, rare, endangered, and threatened plant species. Furthermore, the in-vitro -generated plants had reduced content of therapeutic secondary metabolites compared to their wild counterparts, and the reason behind, not well-explored. Here, we revealed the loss of plant-associated endophytic communities during in-vitro propagation of P. kurrooa plants which also correlated to in-planta secondary metabolite biosynthesis. Therefore, this study emphasized to consider the essential role of plant-associated endophytic communities in in-vitro practices which may be the possible reason for reduced secondary metabolites in in-vitro plants.

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Wild rice harbors more root endophytic fungi than cultivated rice in the F1 offspring after crossbreeding

Cited by 10 publications

References 55 publications

Diversity of endophytic fungal community in Huperzia serrata from different ecological areas and their correlation with Hup A content

Diversity of endophytic fungal community in Huperzia serrata from different ecological areas and their correlation with Hup A content

Diversity of Endophytic Fungal Community in Huperzia Serrata from Different Ecological Areas and Their Correlation with Hup A Content

Endomicrobiome of in vitro and natural plants deciphering the endophytes-associated secondary metabolite biosynthesis in Picrorhiza kurrooa , a Himalayan medicinal herb

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