Background: The excessive and rapid increases in training load (TL) may be responsible for most non-contact injuries in soccer. This study’s aims were to describe, week(w)-by-week, the acute (AW), chronic (CW), acute:chronic workload ratio (wACWR), total distance (wTD), duration training (wDT), sprint total distance (wSTD), repeat sprint (wRS), and maximum speed (wMS) between starter and non-starter professional soccer players based on different periods (i.e., pre-, early-, mid-, and end-season) of a full-season (Persian Gulf Pro League, 2019–2020). Methods: Nineteen players were divided according to their starting status: starters (n = 10) or non-starters (n = 9). External workload was monitored for 43 weeks: pre- from w1–w4; early- from w5–w17; mid- from w18–w30, and end-season from w31–w43. Results: In starters, AW, CW, and wACWR were greater than non-starters (p < 0.05) throughout the periods of early- (CW, p ≤ 0.0001), mid- (AW, p = 0.008; CW, p ≤ 0.0001; wACWR, p = 0.043), or end-season (AW, p = 0.035; CW, p = 0.017; wACWR, p = 0.010). Starters had a greater wTD (p ≤ 0.0001), wSTD (p ≤ 0.0001 to 0.003), wDT (p ≤ 0.0001 to 0.023), wRS (p ≤ 0.0001 to 0.018), and wMS (p ≤ 0.0001) than non-starters during early-, mid-, and end-season. Conclusion: Starters experienced more CW and AW during the season than non-starters, which underlines the need to design tailored training programs accounting for the differences between playing status.