WIN1, a transcriptional activator of epidermal wax accumulation in<i>Arabidopsis</i>

Broun, Pierre; Poindexter, Patricia; Osborne, Erin; Jiang, Cai‐Zhong; Riechmann, José Luis

doi:10.1073/pnas.0305574101

Cited by 369 publications

(385 citation statements)

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“…This constitutive promoter has been used in mutant complementation experiments with a number of cuticular lipid synthesis genes. In particular, a number of genes involved in cuticular lipid biosynthesis and known to be differentially expressed in the epidermis (Suh et al, 2005) were highly induced by 35S-driven overexpression of the WIN1 transcription factor (Broun et al, 2004). Ectopic expression of GPAT5 was confirmed via reverse transcription-PCR transcript analysis of mRNA prepared from seven independent lines using leaves, an organ where GPAT5 is not expressed in wild type (data not shown).…”

Section: Accumulation Of Mags In Root Waxes Correlates With Gpat5 Expmentioning

confidence: 90%

“…In addition, overexpression of AP2 domaincontaining transcription factors (Aharoni et al, 2004;Broun et al, 2004;Zhang et al, 2005) has increased cuticular wax load, but no novel components were reported. This study shows that, with the appropriate biosynthetic gene, it is possible to produce novel cuticular wax components that are transported to the surface.…”

Section: Biotechnology Of Plant Cuticlesmentioning

confidence: 99%

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Monoacylglycerols Are Components of Root Waxes and Can Be Produced in the Aerial Cuticle by Ectopic Expression of a Suberin-Associated Acyltransferase

et al. 2007

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The interface between plants and the environment is provided for aerial organs by epicuticular waxes that have been extensively studied. By contrast, little is known about the nature, biosynthesis, and role of waxes at the root-rhizosphere interface. Waxes isolated by rapid immersion of Arabidopsis (Arabidopsis thaliana) roots in organic solvents were rich in saturated C18-C22 alkyl esters of p-hydroxycinnamic acids, but also contained significant amounts of both a-and b-isomers of monoacylglycerols with C22 and C24 saturated acyl groups and the corresponding free fatty acids. Production of these compounds in root waxes was positively correlated to the expression of sn-glycerol-3-P acyltransferase5 (GPAT5), a gene encoding an acyltransferase previously shown to be involved in aliphatic suberin synthesis. This suggests a direct metabolic relationship between suberin and some root waxes. Furthermore, when ectopically expressed in Arabidopsis, GPAT5 produced very-longchain saturated monoacylglycerols and free fatty acids as novel components of cuticular waxes. The crystal morphology of stem waxes was altered and the load of total stem wax compounds was doubled, although the major components typical of the waxes found on wild-type plants decreased. These results strongly suggest that GPAT5 functions in vivo as an acyltransferase to a glycerol-containing acceptor and has access to the same pool of acyl intermediates and/or may be targeted to the same membrane domain as that of wax synthesis in aerial organs.

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Section: Accumulation Of Mags In Root Waxes Correlates With Gpat5 Expmentioning

confidence: 90%

Section: Biotechnology Of Plant Cuticlesmentioning

confidence: 99%

Monoacylglycerols Are Components of Root Waxes and Can Be Produced in the Aerial Cuticle by Ectopic Expression of a Suberin-Associated Acyltransferase

et al. 2007

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“…Coordinated regulation of metabolic pathways controlling cuticle formation has been demonstrated for transcription factors of the SHINE/WAX-INDUCER family (Aharoni et al, 2004;Broun et al, 2004) and recently for MIXTA-like myeloblastosis (MYB) transcription factors (Oshima et al, 2013). Several of their downstream targets involved in cutin synthesis, modification, and assembly, as well as in cell wall formation and structure and in epidermal cell patterning (Shi et al, 2013), have been identified.…”

mentioning

confidence: 99%

Analyses of Tomato Fruit Brightness Mutants Uncover Both Cutin-Deficient and Cutin-Abundant Mutants and a New Hypomorphic Allele of GDSL Lipase

et al. 2013

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The cuticle is a protective layer synthesized by epidermal cells of the plants and consisting of cutin covered and filled by waxes. In tomato (Solanum lycopersicum) fruit, the thick cuticle embedding epidermal cells has crucial roles in the control of pathogens, water loss, cracking, postharvest shelf-life, and brightness. To identify tomato mutants with modified cuticle composition and architecture and to further decipher the relationships between fruit brightness and cuticle in tomato, we screened an ethyl methanesulfonate mutant collection in the miniature tomato cultivar Micro-Tom for mutants with altered fruit brightness. Our screen resulted in the isolation of 16 glossy and 8 dull mutants displaying changes in the amount and/or composition of wax and cutin, cuticle thickness, and surface aspect of the fruit as characterized by optical and environmental scanning electron microscopy. The main conclusions on the relationships between fruit brightness and cuticle features were as follows: (1) screening for fruit brightness is an effective way to identify tomato cuticle mutants; (2) fruit brightness is independent from wax load variations; (3) glossy mutants show either reduced or increased cutin load; and (4) dull mutants display alterations in epidermal cell number and shape. Cuticle composition analyses further allowed the identification of groups of mutants displaying remarkable cuticle changes, such as mutants with increased dicarboxylic acids in cutin. Using genetic mapping of a strong cutindeficient mutation, we discovered a novel hypomorphic allele of GDSL lipase carrying a splice junction mutation, thus highlighting the potential of tomato brightness mutants for advancing our understanding of cuticle formation in plants.

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“…This genetic switch involves multiple other TFs from diverse families such as the plantspecific APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ ERF) TFs that include major regulators of specific metabolic pathways. WIN1 and members of the SHINE-clade control biosynthesis of cuticular wax in Arabidopsis (Aharoni et al, 2004;Broun et al, 2004;Kannangara et al, 2007). ORA47 activates the expression of the jasmonate (JA) biosynthesis gene LOX3 (Pauwels et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

APETALA2/ETHYLENE RESPONSE FACTOR and basic helix–loop–helix tobacco transcription factors cooperatively mediate jasmonate‐elicited nicotine biosynthesis

et al. 2011

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SUMMARYTranscription factors of the plant-specific apetala2/ethylene response factor (AP2/ERF) family control plant secondary metabolism, often as part of signalling cascades induced by jasmonate (JA) or other elicitors. Here, we functionally characterized the JA-inducible tobacco (Nicotiana tabacum) AP2/ERF factor ORC1, one of the members of the NIC2-locus ERFs that control nicotine biosynthesis and a close homologue of ORCA3, a transcriptional activator of alkaloid biosynthesis in Catharanthus roseus. ORC1 positively regulated the transcription of several structural genes coding for the enzymes involved in nicotine biosynthesis. Accordingly, overexpression of ORC1 was sufficient to stimulate alkaloid biosynthesis in tobacco plants and tree tobacco (Nicotiana glauca) root cultures. In contrast to ORCA3 in C. roseus, which needs only the GCC motif in the promoters of the alkaloid synthesis genes to induce their expression, ORC1 required the presence of both GCC-motif and G-box elements in the promoters of the tobacco nicotine biosynthesis genes for maximum transactivation. Correspondingly, combined application with the JA-inducible Nicotiana basic helix-loop-helix (bHLH) factors that bind the G-box element in these promoters enhanced ORC1 action. Conversely, overaccumulation of JAZ repressor proteins that block bHLH activity reduced ORC1 functionality. Finally, the activity of both ORC1 and bHLH proteins was post-translationally upregulated by a JA-modulated phosphorylation cascade, in which a specific mitogen-activated protein kinase kinase, JA-factor stimulating MAPKK1 (JAM1), was identified. This study highlights the complexity of the molecular machinery involved in the regulation of tobacco alkaloid biosynthesis and provides mechanistic insights about its transcriptional regulators.

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WIN1, a transcriptional activator of epidermal wax accumulation inArabidopsis

Cited by 369 publications

References 50 publications

Monoacylglycerols Are Components of Root Waxes and Can Be Produced in the Aerial Cuticle by Ectopic Expression of a Suberin-Associated Acyltransferase

Monoacylglycerols Are Components of Root Waxes and Can Be Produced in the Aerial Cuticle by Ectopic Expression of a Suberin-Associated Acyltransferase

Analyses of Tomato Fruit Brightness Mutants Uncover Both Cutin-Deficient and Cutin-Abundant Mutants and a New Hypomorphic Allele of GDSL Lipase

APETALA2/ETHYLENE RESPONSE FACTOR and basic helix–loop–helix tobacco transcription factors cooperatively mediate jasmonate‐elicited nicotine biosynthesis

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