During the operation of wind turbines, flow separation appears at the blade roots, which reduces the aerodynamic efficiency of the wind turbine. In order to effectively apply vortex generators (VGs) to blade flow control, the effect of the VG spacing (λ) on flow control is studied via numerical calculations and wind tunnel experiments. First, the large eddy simulation (LES) method was used to calculate the flow separation in the boundary layer of a flat plate under an adverse pressure gradient. The large-scale coherent structure of the boundary layer separation and its evolution process in the turbulent flow field were analyzed, and the effect of different VG spacings on suppressing the boundary layer separation were compared based on the distance between vortex cores, the fluid kinetic energy in the boundary layer, and the pressure loss coefficient. Then, the DU93-W-210 airfoil was taken as the research object, and wind tunnel experiments were performed to study the effect of the VG spacing on the lift-drag characteristics of the airfoil. It was found that when the VG spacing was λ/H = 5 (H represents the VG's height), the distance between vortex cores and the vortex core radius were approximately equal, which was more beneficial for flow control. The fluid kinetic energy in the boundary layer was basically inversely proportional to the VG spacing. However, if the spacing was too small, the vortex was further away from the wall, which was not conducive to flow control. The wind tunnel experimental results demonstrated that the stall angle-of-attack (AoA) of the airfoil with the VGs increased by 10 • compared to that of the airfoil without VGs. When the VG spacing was λ/H = 5, the maximum lift coefficient of the airfoil with VGs increased by 48.77% compared to that of the airfoil without VGs, the drag coefficient decreased by 83.28%, and the lift-to-drag ratio increased by 821.86%.