Wine Metabolite Profiling: Possible Application in Winemaking and Grapevine Breading in Bulgaria

Atanassov, Ilian; Hvarleva, T.; Rusanov, Krasimir; Tsvetkov, I.; Atanassov, A.

doi:10.2478/v10133-009-0011-9

Cited by 15 publications

(6 citation statements)

References 24 publications

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“…The concept of wine omics (Metabolomics: Wine-omics, 2008 ), for the untargeted wine analyses coupling NMR and GC-MS were followed by the oenomics approaches, described by Gougeon et al ( 2009 ), exemplified by the revealing of the metabologeography expression of cooperage oak wood by FTICR-MS. The composition of wine is determined by a complex interplay between environmental factors, genetic factors (grape varieties) and viticultural practices (Atanassov et al, 2009 ; Roullier-Gall et al, 2014a ), and metabolomics offers the toolbox for integrated analyses of the wine composition resulting from all of these factors. Holistic analyses require access to sensitive and powerful instruments, therefore metabolomics typically employs high resolution techniques like FTICR-MS, LC-MS, GC-MS, and NMR spectroscopy combined with multivariate statistics.…”

Section: Introductionmentioning

confidence: 99%

High precision mass measurements for wine metabolomics

et al. 2014

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An overview of the critical steps for the non-targeted Ultra-High Performance Liquid Chromatography coupled with Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-ToF-MS) analysis of wine chemistry is given, ranging from the study design, data preprocessing and statistical analyses, to markers identification. UPLC-Q-ToF-MS data was enhanced by the alignment of exact mass data from FTICR-MS, and marker peaks were identified using UPLC-Q-ToF-MS2. In combination with multivariate statistical tools and the annotation of peaks with metabolites from relevant databases, this analytical process provides a fine description of the chemical complexity of wines, as exemplified in the case of red (Pinot noir) and white (Chardonnay) wines from various geographic origins in Burgundy.

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Section: Introductionmentioning

confidence: 99%

High precision mass measurements for wine metabolomics

et al. 2014

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“…Despite ME being a major issue for the analysis of grape juices and wines, comprehensive metabolite profiling is becoming an important tool these days. This approach has been efficaciously applied to distinguish white wines [95], to observe vintage effects on juices [96] and also to obtain information about grape chemical composition, wine typicity and quality [97]. Howell, et al [98] also used metabolite profiling by GC-MS to determine the connections of different Saccharomyces species during wine fermentation.…”

Section: Application Of Metabolomics In Grape and Wine Research: Statmentioning

confidence: 99%

Grape and Wine Metabolomics to Develop New Insights Using Untargeted and Targeted Approaches

Pinu

2018

Fermentation

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Chemical analysis of grape juice and wine has been performed for over 50 years in a targeted manner to determine a limited number of compounds using Gas Chromatography, Mass-Spectrometry (GC-MS) and High Pressure Liquid Chromatography (HPLC). Therefore, it only allowed the determination of metabolites that are present in high concentration, including major sugars, amino acids and some important carboxylic acids. Thus, the roles of many significant but less concentrated metabolites during wine making process are still not known. This is where metabolomics shows its enormous potential, mainly because of its capability in analyzing over 1000 metabolites in a single run due to the recent advancements of high resolution and sensitive analytical instruments. Metabolomics has predominantly been adopted by many wine scientists as a hypothesis-generating tool in an unbiased and non-targeted way to address various issues, including characterization of geographical origin (terroir) and wine yeast metabolic traits, determination of biomarkers for aroma compounds, and the monitoring of growth developments of grape vines and grapes. The aim of this review is to explore the published literature that made use of both targeted and untargeted metabolomics to study grapes and wines and also the fermentation process. In addition, insights are also provided into many other possible avenues where metabolomics shows tremendous potential as a question-driven approach in grape and wine research.

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“…The integration of biotic and abiotic processes makes foods very complex chemical systems. For example, the chemical complexity of wine combines biochemical impacts, such as plant, microorganism and secondary metabolisms, with environmental and viticultural impacts . Typical small molecular constituents of wine are primary metabolites ( e.g .…”

Section: Introductionmentioning

confidence: 99%

Tandem HILIC‐RP liquid chromatography for increased polarity coverage in food analysis

et al. 2018

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Comprehensive non-targeted analysis of food products normally requires two complementary chromatographic runs to achieve maximum compound coverage. In this study, we present a sensitive tandem-LC method, which combines RP and HILIC separation in a single run. The setup consists of a C18 trap column and two subsequently coupled analytical columns (HILIC and C18) which are operated in parallel. First, hydrophobic compounds are retained on the RP trap column while rather hydrophilic compounds are directly transferred onto a HILIC phase. Next, the pre-fractionated sample composition is analyzed by HILIC or RP chromatography, respectively. The presented setup allows individual and independent gradient elution as well as interfacing with mass spectrometry. The performance of the method has been proven by means of food relevant standards and analysis of complex food samples (e.g. red wine, meat extract). The simple and robust setup provides high flexibility in the selection of column combinations and does not require sophisticated instrumental setups or software. The method significantly increases the covered polarity range compared to classical one-dimensional chromatography. Our results indicate that tandem LC is a valuable and universal tool in the non-targeted screening of various types of complex food samples.

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Wine Metabolite Profiling: Possible Application in Winemaking and Grapevine Breading in Bulgaria

Cited by 15 publications

References 24 publications

High precision mass measurements for wine metabolomics

High precision mass measurements for wine metabolomics

Grape and Wine Metabolomics to Develop New Insights Using Untargeted and Targeted Approaches

Tandem HILIC‐RP liquid chromatography for increased polarity coverage in food analysis

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