Wingless signaling initiates mitosis of primordial germ cells during development in Drosophila

Sato, Takuya; Ueda, Sachie; Niki, Yuzo

doi:10.1016/j.mod.2008.01.004

Cited by 13 publications

(14 citation statements)

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“…Our previous study showed that Wg initiates the reentry of PGCs into mitosis, and Dpp increases the number of female PGCs at the end of the embryonic stage (Sato et al, 2008). Overexpression of Dpp increased the number of PGCs at the pupal-adult transition stage (Zhu and Xie, 2003).…”

Section: Introductionmentioning

confidence: 94%

BMP and Hh Signaling Affects Primordial Germ Cell Division in Drosophila

2010

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The germline is segregated from the remainder of the soma during early embryonic development in metazoan species. In Drosophila, female primordial germ cells (PGCs) continue to proliferate during larval development, and become germline stem cells at the early pupal stage. To elucidate the roles of growth factors in larval PGC division, we examined expression patterns of a bone morphogenetic protein (BMP) growth factor, Decapentaplegic (Dpp), and Hedgehog (Hh), along with factors downstream of each, in the ovary during larval development. Dpp signaling appeared in the ovarian soma from early larval development, and was prominent in the terminal filament cells at late larval stage, whereas Hh appeared in the ovarian soma and PGCs from the third instar larval stage. The number of PGCs decreased when components of these signal transduction pathways were abrogated by RNAi in the PGCs, indicating that both Dpp and Hh signals directly regulate PGC proliferation. Experiments on the up- and down-regulation of Dpp and Hh with a tissue-specific Gal4 driver indicated that Dpp and Hh act as extrinsic and autocrine growth factors. Furthermore, heat-pulse experiments with hs-Gal4 showed that Dpp is active in PGC proliferation throughout larval development, whereas Hh has effects only during late larval development. In addition to Dpp, the reduction of Glass bottom boat (Gbb), another BMP molecule, caused a decrease in the number of PGCs and initiation of larval PGCs differentiation into cystocytes, indicating that Gbb functions to promote PGC division and repress differentiation.

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Section: Introductionmentioning

confidence: 94%

BMP and Hh Signaling Affects Primordial Germ Cell Division in Drosophila

2010

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“…Wingless (WG) is present in PGCs by stage 16 of embryogenesis and WG signaling appears to regulate mitosis of PGCs in the developing male and female gonad (Sato et al, 2008). Thus, cell adhesion and a number of signaling pathways cooperate during formation of the male GSC niche.…”

Section: Male Niche Formationmentioning

confidence: 99%

“…Expression of a reporter of dpp signaling, dad-lacZ, is observed in all PGCs during larval stages and in the anterior PGCs at early pupal stages (Sato et al, 2010;Xie and Spradling, 2000;Zhu and Xie, 2003). Overexpression of dpp in late embryogenesis and during larval development causes an increase in PGC number, while reduction of DPP or a second TGFb ligand, GBB, results in a reduction in PGC number (Sato et al, 2008(Sato et al, , 2010. During early pupal stages, TGFb signaling regulates clonal expansion of GSCs for population of the niche, as mutation of the DPP receptor, Thickveins (TKV), reduces the mitotic potential of GSCs and their ability to clonally populate the niche (Zhu and Xie, 2003).…”

Section: Female Niche Formationmentioning

confidence: 99%

“…Finally, both the Wingless (WG) and Hedgehog (HH) signaling pathways regulate PGC mitosis in females (Sato et al, 2008(Sato et al, , 2010. WG is present in PGCs by stage 16 of embryogenesis, and a reduction in WG signaling results in the delayed entry of PGCs into mitosis (Sato et al, 2008).…”

Section: Female Niche Formationmentioning

confidence: 99%

“…WG is present in PGCs by stage 16 of embryogenesis, and a reduction in WG signaling results in the delayed entry of PGCs into mitosis (Sato et al, 2008). HH signaling is observed in PGCs as early as L3, and a reduction in HH signaling in PGCs also results in a decrease in PGC number (Sato et al, 2010).…”

Section: Female Niche Formationmentioning

confidence: 99%

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Somatic gonadal cells: The supporting cast for the germline

Jemc

2011

Genesis

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Summary:Cell-cell signaling and adhesion are critical for establishing tissue architecture during development and for maintaining tissue architecture and function in the adult. Defects in adhesion and signaling can result in mislocalization of cells, uncontrolled proliferation and improper differentiation, leading to tissue overgrowth, tumor formation, and cancer metastasis. An important example is found in the germline. Germ cells that are not incorporated into the gonad exhibit a greater propensity for forming germ cell tumors, and defects in germline development can reduce fertility. While much attention is given to germ cells, their development into functional gametes depends upon somatic gonadal cells. The study of model organisms has provided great insights into how somatic gonadal cells are specified, the molecular mechanisms that regulate gonad morphogenesis, and the role of germline-soma communication in the establishment and maintenance of the germline stem cell niche. This work will be discussed in the context of Drosophila melanogaster. genesis 49:753-775, 2011. V V C 2011 Wiley Periodicals, Inc.

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