Winogradskyella aurantia sp. nov., isolated from a marine solar saltern

Abstract

A Gram-stain negative, non-flagellated, facultatively anaerobic, slightly halophilic bacterium, WNB302, was isolated from a marine solar saltern in Wendeng, China (122°0'38.85″E, 36°57'56.49″N). Cells of strain WNB302 were 0.2-0.7 µm wide and 2.0-10.0 µm long, catalase- positive and oxidase-negative. Colonies were opaque, orange and approximately 1.0-2.0 mm in diameter after culture for 96 h on marine agar 2216. Growth occurs at 15-37 °C (optimum, 33-35 °C), pH 6.0-8.5 (optimum, pH 7.0-7.5), and with 0.5-7% NaCl (optimum, 2-3% NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain WNB302 belongs to the genus Winogradskyella and is closely related to Winogradskyella exilis KCTC 32356. Strain WNB302 exhibited 96.2 and 96.0% 16S rRNA gene sequence similarities with W. exilis KCTC 32356 and W. litoriviva KCTC 23972. Average nucleotide identity (ANI) value based on draft genomes between strain WNB302 and Winogradskyella thalassocola KCTC 12221 showed a relatedness of 81.1% (with 93.7% of 16S rRNA gene sequence similarity). The major respiratory quinone of strain WNB302 was found to be MK-6, and the dominant fatty acids were found to be iso-C and iso-C G. The major polar lipids of strain WNB302 were three unidentified lipids (L1, L2 and L3), one unidentified aminolipids (AL1) and phosphatidylethanolamine (PE). The genomic DNA G+C content was 37.0 mol%. On the basis of the data presented, strain WNB302 is considered to represent a novel species of the genus Winogradskyella, for which the name Winogradskyella aurantia sp. nov. is proposed. The type strain is WNB302 (= KCTC 52614 = MCCC 1H00172).

Winogradskyella endarachnes sp. nov., a marine bacterium isolated from the brown alga Endarachne binghamiae

“…The genus Winogradskyella is reported to be heterotrophic, Gram-stain-negative and yellow or orange pigmented [5]. Furthermore, the major respiratory quinone for this genus is menaquinone 6 (MK-6) [6]. The present manuscript reports the isolation and characterization of a novel species of the genus Winogradskyella obtained from a brown alga.…”

Paucihalobacter ruber gen. nov., sp. nov., isolated from a haloalkaline lake sediment sample

“…aquatile LMG 4008 T [15] and W. aurantia WNB302 T [16] were used as the reference strains to compare with strain CWB-1 T .…”

“…The corresponding information of W. aurantia WNB302 T is from Sun et al [16]. The Gram-staining was performed as described by Claus et al [27].…”

“…Strain CWB-1 T and the reference strains all had iso-C 15 : 0 as the predominant fatty acids. Nevertheless, one of the major fatty acids (>10 %) of strain CWB-1 T was identified as summed feature 1 (iso-C 15 : 1 H/C 13 : 0 3-OH; 24.2 %), which was not found in the three reference strains of PAMC 27130 T , ACAM 188 T and LMG 4008 T , and was not present in WNB302 T [16]. In contrast, iso-C 15 : 1 G was found in related strains, but was not detected in strain CWB-1 T .…”

Marinilabilia rubra sp. nov., isolated from a marine solar saltern

“…Physiological parameters of the marine solar saltern were measured in situ : salinity, 70‰, total dissolved solids, 19.1 mg l −1 ; and conductivity, 38.1 S m –1 . Our laboratory has described five novel species from this marine solar saltern: Bacillus mesophilus Zhou et al 2016 [7], Winogradskyella aurantia Sun et al 2017 [8], Lentibacillus sediminis Guo et al 2017 [9], Roseovarius salinarum Wang et al 2018 [10] and Rhodosalinus sediminis Guo et al 2017 [11]. Cultivable bacterial strains were isolated using a standard dilution plating technique at 30 °C on marine agar 2216 (MA; BD).…”