Winogradskyella haliclonae sp. nov., isolated from a marine sponge of the genus Haliclona

Abstract

A yellow-pigmented, Gram-stain-negative, motile and rod-shaped bacterium, strain M1A16, was isolated from the internal tissue of a sponge of the genus Haliclona, which was long-term cultured in the CEMarin aquaria system at Justus Liebig University of Giessen. The strain grew well at 20-32 °C (optimum 25 °C), in the presence of 0-6 % NaCl (optimum 3 %), and at pH 5.5-9.0 (optimum pH 7.0-8.0). Phylogenetic analysis based on its 16S rRNA gene sequence placed the strain within the monophyletic cluster of the genus Winogradskyella with highest sequence similarity to Winogradskyella jejuensis CP32 (98.3 % 16S rRNA gene sequence similarity). Sequence similarities to all other type strains were 98.0 % or less. DNA-DNA hybridization of strain M1A16 with W. jejuensis CP32 resulted in hybridization values of 44.1 % (reciprocal 68.1 %). Major cellular fatty acids of strain M1A16 were iso-C15 : 1 G (18.1 %), iso-C15 : 0 (13.7 %), C16 : 1ω7c (12.9 %), iso-C17 : 0 3-OH (10.6 %) and iso-C16 : 0 3-OH (10.2 %). The overall polyamine content was very low with major components being cadaverine, spermidine and sym-homospermidine. The major quinone was menaquinone MK-6. The polar lipid profile contained predominantly phosphatidylethanolamine, two unidentified aminolipids and two unidentified lipids devoid of a detectable functional group. The genomic DNA G+C content was 32.7 mol%. Based on the phylogenetic, chemotaxonomic and phenotypic analyses, strain M1A16 represents a novel species of the genus Winogradskyella, for which the name Winogradskyella haliclonae sp. nov. is proposed. The type strain is M1A16 (=DSM 103138=CCM 8681=LMG 29588=CIP 111091).

Variability in the Composition of Pacific Oyster Microbiomes Across Oyster Families Exhibiting Different Levels of Susceptibility to OsHV-1 μvar Disease

“…OTUs assigned to the Bradyrhizobiaceae family have previously been observed in oysters (Sakowski, 2015), however, due to the coarse taxonomic assignment of this OTU, it is unclear what potential role this member of the Bradyrhizobiaceae family might have. Winogradskyella species are commonly found in numerous marine organisms, including oysters (Valdenegro-Vega et al, 2013; Park et al, 2015; Lee et al, 2017; Schellenberg et al, 2017; Franco et al, 2018), and are known for their role in amoebic-induced fish gill diseases (Embar-Gopinath et al, 2005, 2006). However, it is uncertain what function(s) Winogradskyella species play in oysters.…”

Sea foams are ephemeral hotspots for distinctive bacterial communities contrasting sea-surface microlayer and underlying surface water

“…1987), disadvantages might be easily outweighed by benefits of particles providing organic material and shelter from extreme levels of ultraviolet and solar radiation experienced at the air-sea boundary. The LefSe analysis revealed that Winogradskyella ( Flavobacteriaceae ), often being associated with brown algae or sponges (Park and Yoon 2013; Schellenberg, et al . 2017; Yoon and Lee 2012), was abundant among the metabolically active OTUs in foams (Figure 6).…”

“…Although attachment to particles might have some drawbacks for bacteria regarding grazing (Albright, et al 1987), disadvantages might be easily outweighed by benefits of particles providing organic material and shelter from extreme levels of ultraviolet and solar radiation experienced at the air-sea boundary. The LefSe analysis revealed that Winogradskyella (Flavobacteriaceae), often being associated with brown algae or sponges (Park and Yoon 2013;Schellenberg, et al 2017; Yoon and Lee 2012), was abundant among the metabolically active OTUs in foams ( Figure 6). As broken algal cells and detritus are major parts of foams, high relative abundance of Winogradskyella in the foam particle-associated fraction ( Supplementary Figure 11) might be due to its attachment to algal-derived substrates.…”

Winogradskyella ouciana sp. nov., isolated from the hadal seawater of the Mariana Trench

“…S4). The sole respiratory quinone of strain ZXX205 T was MK-6, which is also the major respiratory quinone in the most members of the genus Winogradskyella [2, 7–12]. The DNA G+C content of ZXX205 T was 35.5 %, a value in the range reported for recognized species of the genus Winogradskyella (30.1~39.0 mol%) [1–4, 7–12].…”

“…Within the genus Winogradskyella, all members have been isolated from marine environments or marine organisms [7], strictly aero-bic or facultatively anaerobic and catalase-positive. Besides W. psychrotolerans (the respiratory quinones of this species are MK-6 and MK-7) [6], the sole or major respiratory quinone of the species in genus Winogradskyella is MK-6 [2,[7][8][9][10][11][12]. The main cellular fatty acids are branched-chain (iso-C 15 : 0 , iso-C 15 : 1 , anteiso-C 15 : 0 ) and fatty acids with hydroxy groups (iso-C 15 : 0 3-OH, iso-C 16 : 0 3-OH, and iso-C 17 : 0 3-OH) [10,12].…”