Wire templated electrodeposition of vessel-like structured chitosan hydrogel by using a pulsed electrical signal

Yan, Kun; Yang, Chenguang; Zhong, Weibin; Lu, Zhentan; Li, Xiufang; Shi, Xiaowen; Wang, Dong

doi:10.1039/d0sm01134g

Cited by 18 publications

(17 citation statements)

References 31 publications

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“…By electronically controlling the amount of generated charges, the reaction leading to hydrogel synthesis can be precisely controlled so that a specific amount of the biohybrid material (PEG-heparin hydrogel) can be synthesized on the electrode surface. [38] We used a hydrogel containing heparin, a highly sulfated GAG because it resembles the heparan sulfate of extracellular matrices. The native extracellular matrix is rich in GAGs which complex multiple soluble signalling proteins (cytokines and growth factors) and thereby modulate their spatiotemporal distribution, stability, and activity.…”

Section: Resultsmentioning

confidence: 99%

Electrically Controlled Click‐Chemistry for Assembly of Bioactive Hydrogels on Diverse Micro‐ and Flexible Electrodes

Silva¹,

Akbar²,

Paterson³

et al. 2022

Macromol. Rapid Commun.

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The seamless integration of electronics with living matter requires advanced materials with programmable biological and engineering properties. Here electrochemical methods to assemble semi-synthetic hydrogels directly on electronically conductive surfaces are explored. Hydrogels consisting of poly (ethylene glycol) (PEG) and heparin building blocks are polymerized by spatially controlling the click reaction between their thiol and maleimide moieties. The gels are grown as conformal coatings or 2D patterns on ITO, gold, and PtIr. This study demonstrates that such coatings significantly influence the electrochemical properties of the metal-electrolyte interface, likely due to space charge effects in the gels. Further a promising route toward engineering and electrically addressable extracellular matrices by printing arrays of gels with binary cell adhesiveness on flexible conductive surfaces is highlighted.

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Section: Resultsmentioning

confidence: 99%

Electrically Controlled Click‐Chemistry for Assembly of Bioactive Hydrogels on Diverse Micro‐ and Flexible Electrodes

Silva¹,

Akbar²,

Paterson³

et al. 2022

Macromol. Rapid Commun.

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show abstract

“…When an oscillating electrical input was imposed, a segmented output structure was generated, and this illustrates the broader point that complex electrical inputs can be used to generate information-containing patterns in supramolecular structure. Importantly, the pattern can be erased and the medium dissolved by treating with acid and heat, while cooling regenerates a medium that can be reprogrammed with another pattern. ,, …”

Section: Electro-biofabrication: From Cuing Self-assembly To Controll...mentioning

confidence: 99%

Electro-Biofabrication. Coupling Electrochemical and Biomolecular Methods to Create Functional Bio-Based Hydrogels

et al. 2023

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Twenty years ago, this journal published a review entitled "Biofabrication with Chitosan" based on the observations that (i) chitosan could be electrodeposited using low voltage electrical inputs (typically less than 5 V) and (ii) the enzyme tyrosinase could be used to graft proteins (via accessible tyrosine residues) to chitosan. Here, we provide a progress report on the coupling of electronic inputs with advanced biological methods for the fabrication of biopolymer-based hydrogel films. In many cases, the initial observations of chitosan's electrodeposition have been extended and generalized: mechanisms have been established for the electrodeposition of various other biological polymers (proteins and polysaccharides), and electrodeposition has been shown to allow the precise control of the hydrogel's emergent microstructure. In addition, the use of biotechnological methods to confer function has been extended from tyrosinase conjugation to the use of protein engineering to create genetically fused assembly tags (short sequences of accessible amino acid residues) that facilitate the attachment of function-conferring proteins to electrodeposited films using alternative enzymes (e.g., transglutaminase), metal chelation, and electrochemically induced oxidative mechanisms. Over these 20 years, the contributions from numerous groups have also identified exciting opportunities. First, electrochemistry provides unique capabilities to impose chemical and electrical cues that can induce assembly while controlling the emergent microstructure. Second, it is clear that the detailed mechanisms of biopolymer self-assembly (i.e., chitosan gel formation) are far more complex than anticipated, and this provides a rich opportunity both for fundamental inquiry and for the creation of high performance and sustainable material systems. Third, the mild conditions used for electrodeposition allow cells to be co-deposited for the fabrication of living materials. Finally, the applications have been expanded from biosensing and lab-on-a-chip systems to bioelectronic and medical materials. We suggest that electro-biofabrication is poised to emerge as an enabling additive manufacturing method especially suited for life science applications and to bridge communication between our biological and technological worlds.

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“…Even though the electrodeposition of chitosan can be well controlled experimentally and has been monitored optically on planar and cylindrical electrodes by Payne et al [26][27][28], the quantification of its kinetics is not straightforward. For the ease of treatment, one may look at the deprotonation/protonation of the amino group, which is the driving force of chitosan gelation/dissolution:…”

Section: Step 1: Approachingmentioning

confidence: 99%

Rational shaping of hydrogel by electrodeposition under fluid mechanics for electrochemical writing on complex shaped surfaces at microscale

Helú¹,

Liu²

2021

Chemical Engineering Journal

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Wire templated electrodeposition of vessel-like structured chitosan hydrogel by using a pulsed electrical signal

Abstract: Herein, by performing the templated electrodepsotion process with the oscillating electrical signal stimulation, a vessel-like structured chitosan hydrogel (diameter about 0.4 mm) was successfully prepared in the absence of salt...

Cited by 18 publications

References 31 publications

Electrically Controlled Click‐Chemistry for Assembly of Bioactive Hydrogels on Diverse Micro‐ and Flexible Electrodes

Electrically Controlled Click‐Chemistry for Assembly of Bioactive Hydrogels on Diverse Micro‐ and Flexible Electrodes

Electro-Biofabrication. Coupling Electrochemical and Biomolecular Methods to Create Functional Bio-Based Hydrogels

Rational shaping of hydrogel by electrodeposition under fluid mechanics for electrochemical writing on complex shaped surfaces at microscale

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