The physiological aspects of the implementation of the cow milk transfer reflex during machine milking are the result of exposure to the threshold force of a given value of a variable vacuum and simultaneous mechanical irritation by the teat rubber of the udder of the cow udder system. This effect leads to the discovery of sphincters with the subsequent release of the hormone oxytocin by the pituitary gland and the milk accumulation from the alveolar udder tissue. Signal environmental factors (sounds of a vacuum pump, milking machines, feeder) cause reactions in cows not only by irritation but also by frequency. It has been established that exposure to a high-frequency electromagnetic field destroys most of the common causative agents of mastitis-staphylococci, streptococci. For the treatment of mastitis, stimulation of lactation and the reflex of milk flow in cows, it is promising to apply the effect of a high frequency electromagnetic field modulated by radio frequencies, which contribute to the conversion of electromagnetic field energy into energy of nerve impulses and chemical reactions of mitochondrial cellular mechanisms of udder alveolar tissue cells. The use of alternating sets of specific frequencies of a modulating electromagnetic field of high frequency allows the alternation of “killer” frequencies for microbes with stimulating secretion of alveolar cells of the udder and with frequencies that act anti-inflammatory. It is essential to determine the frequencies that create protective inhibition from exposure to extreme irritations of milking factors (increased vacuum, durable worn nipple rubber). With prolonged exposure to an electromagnetic field, such frequencies a repeating conditioned stimulus of a weak strength, which precede and accompany the impact of the milking machine, and stimulate the activity of the nerve centers of the milk yield reflex in cows, creating a dominant lactation.