Wirelessly powered and remotely controlled valve-array for highly multiplexed analytical assay automation on a centrifugal microfluidic platform

Delgado, Saraí M. Torres; Kinahan, David J.; Julius, Lourdes Albina Nirupa; Mallette, Adam; Ardila, David Sáenz; Mishra, Rohit; Miyazaki, Celina M.; Korvink, Jan G.; Ducrée, Jens; Mager, Dario

doi:10.1016/j.bios.2018.03.012

Cited by 49 publications

(36 citation statements)

References 65 publications

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“…In (Torres Delgado et al, 2016), we demonstrated continuous on-disc readout of a chemiluminescent signal by fitting a silicon photo-multiplier (ASD-NUV1S-P from AdvanSiD) and the necessary amplification stage circuitry (a logarithmic amplifier, LOG112 from Texas Instruments) onto an ApplicationDisc, showing an accuracy higher than 96% when compared to a commercial benchtop luminometer (GloMax luminometer from Promega Corporation). In (Torres Delgado et al, 2018) we demonstrated how to integrate and automate up to 128 thermally actuated valves on-disc. These were individually activated by executing a predefined timed sequence, or triggered, based on mixing and separation states, the variables being monitored in our case.…”

Section: Experiments and Resultsmentioning

confidence: 99%

“…For example, depending on the actual status of a particular variable state (eg. mixing, incubation, or sedimentation), a particular action may need to be initiated based on the analyst's opinion (Torres Delgado et al, 2018). Hence it is desirable to facilitate wireless and real-time interaction with the platform and the ongoing experiment, via the implementation of a bidirectional link.…”

Section: Module Iv: Low Energy Bluetoothmentioning

confidence: 99%

“…2(f) shows user interfaces programmed for the activation of 128 valves, able to connect and interact with the eLoaD platform, which makes itself available by waiting for incoming connection requests. After the pairing request is accepted and the two devices exchange security keys and complete the connection process, the user can then start activating and deactivating valves either in manual or automatic control fashion as described in (Torres Delgado et al, 2018).…”

Section: Interfacingmentioning

confidence: 99%

“…Interfacing √ Almost any assay (eg. on demand and automatic valve actuation (Torres Delgado et al, 2018)).…”

Section: Pumping * √ √mentioning

confidence: 99%

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The eLoaD platform endows centrifugal microfluidics with on-disc power and communication

Delgado

Korvink

Mager

2018

Biosensors and Bioelectronics

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In this paper we present a comprehensive description of the design, fabrication and operation of an electrified Lab-on-a-Disc (eLoaD) system. The smart platform is developed to extend conventional Lab-on-a-Disc applications with an electronic interface, providing additional flow control and sensing capabilities to centrifugal microfluidics platforms. Wireless power is transferred from a Qi-compliant transmitter to the eLoaD platform during rotation. An Arduino-based microcontroller, a Bluetooth communication module, and an on-board SD-card are integrated into the platform. This generalises the applicability of the eLoaD and its modules for performing a wide range of laboratory unit operations, procedures, or diagnostic assays, all controlled wirelessly during spinning. The lightweight platform is fully reusable and modular in design and construction. An interchangeable and non-disposable application disc is fitted with the necessary sensors and/or actuators for a specific assay or experiment to be performed. A particular advantage is the ability to continuously monitor and interact with LoaD experiments, overcoming the limitations of stroboscopy. We demonstrate the applicability of the platform for two sensing experiments involving optical, electrochemical, and temperature detection, and one actuation experiment involving controlled heating/cooling, and flow control with active valves. The complete electronic designs and example programming codes are extensively documented in the supplementary material for easy adaptation.

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Section: Experiments and Resultsmentioning

confidence: 99%

Section: Module Iv: Low Energy Bluetoothmentioning

confidence: 99%

Section: Interfacingmentioning

confidence: 99%

“…Interfacing √ Almost any assay (eg. on demand and automatic valve actuation (Torres Delgado et al, 2018)).…”

Section: Pumping * √ √mentioning

confidence: 99%

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The eLoaD platform endows centrifugal microfluidics with on-disc power and communication

Delgado

Korvink

Mager

2018

Biosensors and Bioelectronics

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“…These lab-on-a-disc systems make specific use of the interplay of the frequency controlled centrifugal force and capillary action in a surface-functionalized network of micro-channels and cavities. Key liquid handling operations such as valving [44][45][46][47][48], metering [49,50], mixing [51,52], pumping [53][54][55], and switching/routing of flow [56,57] have been demonstrated.A major challenge for microfluidic systems is rapid mixing. In general, one can categorize micromixers according to different characteristics [58,59], e.g., batch and continuous flow mode, laminar and turbulent, planar and 3-dimensional geometry, low-or high-aspect-ratio structures, manufacturing technologies or active/passive mixers.…”

mentioning

confidence: 99%

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

Burger¹,

Kinahan

Cayron

et al. 2019

Inventions

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We present a powerful and compact batch-mode mixing and dilution technique for centrifugal microfluidic platforms. Siphon structures are designed to discretize continuous flows into a sequence of droplets of volumes as low as 100 nL. Using a passive, self-regulating 4-step mechanism, discrete volumes of two fluids are alternatingly issued into a common intermediate chamber. At its base, a capillary valve acts as a fluidic shift register; a single droplet is held in place while two or more droplets merge and pass through the capillary stop. These merged droplets are advectively mixed as they pass through the capillary valve and into the receiving chamber. Mixing is demonstrated for various combinations of liquids such as aqueous solutions as well as saline solutions and human plasma. The mixing quality is assessed on a quantitative scale by using a colorimetric method based on the mixing of potassium thiocyanate and iron(III) chloride, and in the case of human plasma using a spectroscopic method. For instance, volumes of 5 µL have been mixed in less than 20 s. Single-step dilutions up to 1:5 of plasma in a standard phosphate buffer solution are also demonstrated. This work describes the preliminary development of the mixing method which has since been integrated into a commercially available microfluidic cartridge.Inventions 2020, 5, 1 2 of 14 microfluidic systems constitute a subset which uses the rotation of the chip, which is typically of a similar geometry as a Compact Disc™ or DVD™, to enable most pumping and valving operations. These systems can be further sub-divided into 'Bioanalytical Screening CDs' and 'Lab-on-a-Disc (LoaD)'. Bioanalytical screening CDs focus on adapting optical disc drive (ODD) technology (e.g., Compact Disc, DVD) for biological detection [9]. This technology takes advantage of the high-density data storage capability as well as the favorable pricing for its main constituents, i.e., the optical pickup unit, the spindle drive, and the optical disc substrates [10][11][12][13][14].The LoaD [15][16][17][18][19][20] is typically used to automate the standard laboratory steps conducted in a biology lab to enable a fully automated sample-to-answer system. The LoaD is particularly applicable for deployment in the field (point-of-use) or at the hospital bed-side (point-of-care) as the chips can be loaded at atmospheric pressure (via pipette or syrette) without issues regarding sealing or priming. The use of centrifugation for pumping also reduces the cost and complexity of support instrumentation as just low-cost spindle motors are required rather than specialized micropumps. Similarly, inherent centrifugation of samples [21][22][23] applied to sample preparation is a particular strength.The technological precursor of these systems are the centrifugal analyzers which became popular in the 1970s and 1980s [24] for automating a very limited number of simple assay steps, on rather macroscopic sample volumes, and readout on a rotor-based instrument. Currently the main areas of application are immuno...

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An Open Microfluidic Chip for Continuous Sampling of Solute from a Turbulent Particle Suspension

et al. 2020

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High solids content complicates in situ analysis of chemical processing, biological suspensions, and environmental streams. In most cases, analytical methods require at least one pre‐treatment step of a small volume of sample before a particle‐free fluid can be analyzed. We have developed a continuous in situ sampler that can “sip” particle‐free solution from a turbulent high solids content stream (a slurry). An open microfluidic chip with an extended slit opening shields the internal laminar flow from the turbulence outside. Unlike other open chips, our chip does not require close proximity to a solid surface and operates in turbulent environments for hours without maintenance. Two applications are demonstrated: monitoring FeIII in a stirred slurry of mixed ore particles at high solids loading (4 %wt) and paracetamol tablet dissolution profiles for two different formulations.

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Wirelessly powered and remotely controlled valve-array for highly multiplexed analytical assay automation on a centrifugal microfluidic platform

Cited by 49 publications

References 65 publications

The eLoaD platform endows centrifugal microfluidics with on-disc power and communication

The eLoaD platform endows centrifugal microfluidics with on-disc power and communication

Siphon-Induced Droplet Break-Off for Enhanced Mixing on a Centrifugal Platform

An Open Microfluidic Chip for Continuous Sampling of Solute from a Turbulent Particle Suspension

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