Wirkung von 5-Hydroxytryptamin auf die isolierte Nabelschnurarterie

Rodegra, H; Röder, Georg; Schmermund, H. J.; Soehring, K

doi:10.1007/bf00259874

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several biologically active substances have been suggested to play a role in fetal circulation and to be involved in closure of the umbilical blood vessels at birth, i.e., serotonin [4,9], bradykinin [2,8] and prostaglandins [5][6][7], The actions of both serotonin and bradykinin have been studied on isolated sheep umbilical blood vessels [1], In these studies serotonin was found to be a good agonist while bradykinin failed to contract sheep umbil ical arteries or veins. The lack of effect produced by bradykinin on sheep umbilical vessels thus differed from that reported for human umbilical vessels [2].…”

Section: Discussionmentioning

confidence: 99%

Comparison of the Constricting Actions Produced by Serotonin and Prostaglandins on Isolated Sheep Umbilical Arteries and Veins

Dc¹

1970

Gynecol Obstet Invest

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of the Constricting Actions Produced by Serotonin and Prostaglandins on Isolated Sheep Umbilical Arteries and Veins

Dc¹

1970

Gynecol Obstet Invest

View full text Add to dashboard Cite

show abstract

“…The importance of the HUA has long been recognised, and its use in research dates back to the 1950s. 3 Umbilical cord is one of the few human tissues that is widely available and provides a high yield of HUA preparations.…”

Section: Introductionmentioning

confidence: 99%

Time-dependent and force-dependent vasoreactivity of isolated human umbilical arteries

Đukanović¹,

M²,

Škrbić³

2020

Scripta Medica

View full text Add to dashboard Cite

Background/Aim: There have been different experimental conditions for in vitro studies on human umbilical arteries (HUA) in tissue bath system. This diversity was mainly reflected in variables such as stretching tension, incubation period and initial constriction challenging with potassium (KCl). The aim of the study was to establish optimal experimental conditions which will provide better responsiveness of HUA preparations, as well as to examine the impact of 24 h cold storage on viability and responsiveness of HUA to KCl and serotonin. Methods: The KCl-induced constrictions at different stretching tensions (0.5 g, 1.0 g, 2.0 g, 4.0 g), incubation times (30 min, 60 min, 120 min), and after multiple initial constriction challenging were compared. Dose response curves for serotonin were obtained under different conditions (1.0 g and 60 min vs. 2.0 g and 120 min). The influence of 24 h cold storage on KCland serotonininduced vasoconstriction of HUA preparations was examined as well. Results: The strongest constrictions induced by serotonin or KCl were obtained when preparations were adjusted at 2.0 g and incubated for 120 min. The KCl-induced constrictions observed after 120 min were statistically higher (p < 0.05) when preparations were challenged three times (30 min, 60 min, 120 min), compared to those challenged only once. The preparations that were stored at 4 ⁰C for 24 h showed significantly stronger serotonin-induced constrictions (p < 0.01). The cold storage had no influence on KCl-induced constriction. Conclusion: For performing in vitro studies on HUA preparations in tissue bath, we propose stretching tension of 2.0 g, incubation period of 120 min and multiple initial constriction challenging with KCl as optimal experimental condition. We also showed that HUA preparations retained functional viability even after 24 h of cold storage.

show abstract

Wirkung von 5-Hydroxytryptamin auf die isolierte Nabelschnurarterie

Cited by 2 publications

References 0 publications

Comparison of the Constricting Actions Produced by Serotonin and Prostaglandins on Isolated Sheep Umbilical Arteries and Veins

Comparison of the Constricting Actions Produced by Serotonin and Prostaglandins on Isolated Sheep Umbilical Arteries and Veins

Time-dependent and force-dependent vasoreactivity of isolated human umbilical arteries

Contact Info

Product

Resources

About