Wisteria Floribunda Agglutinin-Labeled Perineuronal Nets in the Mouse Inferior Colliculus, Thalamic Reticular Nucleus and Auditory Cortex

Fader, Sarah; Imaizumi, Kazuo; Yanagawa, Yuchio; Lee, Charles C.

doi:10.3390/brainsci6020013

Cited by 18 publications

(26 citation statements)

References 54 publications

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“…However, a number of PNN+ cells were devoid of PV staining and vice versa . These observations were consistent across the mouse strains and age (see Figure 2) and with previous studies of PNN expression in young mouse A1 (Happel et al, 2014; Fader et al, 2016). …”

Section: Resultssupporting

confidence: 91%

“…The relationship between PV and PNN expression in A1 has not been previously characterized. Consistent with previous articles on mouse A1, PNN expression was seen in layers II–VI, with a particularly high concentration in layer IV (Happel et al, 2014; Fader et al, 2016). The PNN+ cell density was similar in C57 and CBA mice.…”

Section: Discussionsupporting

confidence: 91%

“…Many PV+ cells in A1 are ensheathed by PNN (Happel et al, 2014; Fader et al, 2016). PNNs are specialized extracellular matrix components and consist of chondroitin sulfate proteoglycans (CSPG) that are found throughout the extracellular matrix, but are highly dense around PV+ interneurons.…”

Section: Introductionmentioning

confidence: 99%

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Age-Related Deterioration of Perineuronal Nets in the Primary Auditory Cortex of Mice

Brewton

Kokash

Jimenez

et al. 2016

Front. Aging Neurosci.

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Age-related changes in inhibitory neurotransmission in sensory cortex may underlie deficits in sensory function. Perineuronal nets (PNNs) are extracellular matrix components that ensheath some inhibitory neurons, particularly parvalbumin positive (PV+) interneurons. PNNs may protect PV+ cells from oxidative stress and help establish their rapid spiking properties. Although PNN expression has been well characterized during development, possible changes in aging sensory cortex have not been investigated. Here we tested the hypothesis that PNN+, PV+ and PV/PNN co-localized cell densities decline with age in the primary auditory cortex (A1). This hypothesis was tested using immunohistochemistry in two strains of mice (C57BL/6 and CBA/CaJ) with different susceptibility to age-related hearing loss and at three different age ranges (1–3, 6–8 and 14–24 months old). We report that PNN+ and PV/PNN co-localized cell densities decline significantly with age in A1 in both mouse strains. In the PNN+ cells that remain in the old group, the intensity of PNN staining is reduced in the C57 strain, but not the CBA strain. PV+ cell density also declines only in the C57, but not the CBA, mouse suggesting a potential exacerbation of age-effects by hearing loss in the PV/PNN system. Taken together, these data suggest that PNN deterioration may be a key component of altered inhibition in the aging sensory cortex, that may lead to altered synaptic function, susceptibility to oxidative stress and processing deficits.

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Section: Resultssupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

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Age-Related Deterioration of Perineuronal Nets in the Primary Auditory Cortex of Mice

Brewton

Kokash

Jimenez

et al. 2016

Front. Aging Neurosci.

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“…Given the ability of some neurons within the MGB to synchronize to temporally varying stimuli presented at high repetition rates, it might be expected that PNNs would be associated with MGB neurons, particularly those in the medial MGB. However, very few studies have assessed PNN expression in the MGB, and those that have, report that PNN expression is largely absent from the MGB (Beebe & Schofield, 2018;Fader, Imaizumi, Yanagawa, & Lee, 2016;Friauf, 2000;Gáti et al, 2010). Within the auditory system, genetic and pharmacological degradation of PNNs leads to a reduction in sound-evoked firing rates and increased neuronal response thresholds in the brainstem (Balmer, 2016;Blosa et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Auditory temporal acuity improves with age in the male mouse auditory thalamus: A role for perineuronal nets?

Quraishe

Newman

Anderson³

2019

J of Neuroscience Research

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The ability to perceive and interpret environmental sound accurately is conserved across many species and is fundamental for understanding communication via vocalizations. Auditory acuity and temporally controlled neuronal firing underpin this ability. Deterioration in neuronal firing precision likely contributes to poorer hearing performance, yet the role of neural processing by key nuclei in the central auditory pathways is not fully understood. Here, we record from the auditory thalamus (medial geniculate body [MGB]) of young and middle‐aged, normally hearing male CBA/Ca mice. We report changes in temporal processing of auditory stimuli, with neurons recorded from ventral and medial MGB subdivisions of older animals more likely to synchronize to rapid temporally varying stimuli. MGB subdivisions also showed increased probability of neuronal firing and shorter response latencies to clicks in older animals. Histological investigation of neuronal extracellular specializations, perineuronal nets (PNNs) and axonal coats, in the MGB identified greater organization of PNNs around MGB neurons and the presence of axonal coats within older animals. This supports the observation that neural responses recorded from ventral and medial MGB of older mice were more likely to synchronize to temporally varying stimuli presented at faster repetition rates than those recorded from young adult animals. These changes are observed in animals with normal hearing thresholds, confirming that neural processing differs between the MGB subdivisions and such processing is associated with age‐related changes to PNNs. Understanding these age‐related changes and how they occur have important implications for the design of effective therapeutic interventions to improve speech intelligibility into later life.

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“…in the primary auditory cortex [17]. Thus, the inhibitory synaptic plastic changes are not associated with the PNNs in the inferior colliculus.…”

Section: Discussionmentioning

confidence: 87%

Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex

Park

Lee

et al. 2020

BMC Neurosci

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Background: This study aimed to investigate the changes in molecules related to perineuronal nets (PNNs) and synaptic transporters in the primary auditory cortices of rats with noise-induced hearing loss. Female Sprague-Dawley rats at postnatal day 7 were divided into the noise and control groups. Four hours of 115 dB SPL white noise was delivered for 10 days to the noise group. Thirty days after noise exposure, the primary auditory cortex and the inferior colliculus were harvested. The expression levels of vesicular glutamatergic transporter (VGLUT)1, VGLUT2, vesicular GABA transporter (VGAT), glutamate decarboxylase (GAD)67, brevican, aggrecan, MMP9, and MMP14 were evaluated using real-time reverse transcription polymerase chain reaction or western blot. An immunofluorescence assay was conducted to assess parvalbumin (PV), Wisteria floribunda agglutinin (WFA), and brevican. The immune-positive cells were counted in the primary auditory cortex. Results: The expression level of VGLUT1 in the primary auditory cortex was decreased in the noise group. The expression level of VGLUT2 in the inferior colliculus was elevated in the noise group. The expression levels of brevican and PV + WFA in the primary auditory cortex were decreased in the noise group. The expression level of MMP9 in the primary auditory cortex was increased in the noise group. Conclusion: Noise-induced hearing loss during the precritical period impacted PNN expression in the primary auditory cortex. Increased MMP9 expression may have contributed to the decrease in brevican expression. These changes were accompanied by the attenuation of glutamatergic synaptic transporters.

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Wisteria Floribunda Agglutinin-Labeled Perineuronal Nets in the Mouse Inferior Colliculus, Thalamic Reticular Nucleus and Auditory Cortex

Cited by 18 publications

References 54 publications

Age-Related Deterioration of Perineuronal Nets in the Primary Auditory Cortex of Mice

Age-Related Deterioration of Perineuronal Nets in the Primary Auditory Cortex of Mice

Auditory temporal acuity improves with age in the male mouse auditory thalamus: A role for perineuronal nets?

Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex

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