Withania somnifera (Ashwagandha) root extract counteract acute and chronic impact of γ-radiation on liver and spleen of rats

Abstract

The exposure to ionizing radiation has become inescapably because of increased dependence on radiation to execute works in different fields and also its influences on biological systems. Thus, the current study aimed at examination of the radio-protective effect of the natural ashwagandha (Ag) against acute and chronic doses of γ-radiation on liver and spleen of rats. The impact of Ag was inspected in rats exposed to acute exposure of 8 Gy (single dose) or to chronic exposure of 8 Gy (2 Gy every other day for 4 times). The data obtained reveals significant amelioration of the redox status (MDA, GSH and ROS) in spleen and liver tissues of rats treated with Ag and exposed to the 2-different modes of γ-radiation. Besides, the changes in inflammatory responses assessed by measurements of IL-17, IL-10 and α7-nAchR are less pronounced in rats received Ag and γ-radiation compared to irradiated rats. Further, the measurements of tissues structural damage markers (MMP-2, MMP-9 and TIMP-1) pointed to benefit of Ag against γ-radiation. The histopathological investigation of spleen and liver tissues confirmed this ameliorating action of Ag counter to γ-radiation hazards. It could be suggested that Ashwagandha could exerts radio-protective influences because of its antioxidants and anti-inflammatory capabilities.

Ondansetron or beta-sitosterol antagonizes inflammatory responses in liver, kidney, lung and heart tissues of irradiated arthritic rats model

“…The present study found that exposing arthritic rats to fractionated whole body γ-irradiation (2 Gy/fraction for four consecutive weeks; A + R group) led to marked deterioration in the clinical arthritic signs, biochemical indices of oxidative stress and inflammation in liver, kidney, lung, and heart tissues ( Figure 5 ), in addition to degenerative lesions in the ankle joints which are in line with the study of Thabet et al 73 The observed results may be ascribed to an excessive generation of reactive oxygen species (ROS). This generates oxidative damage to biomolecules, linked to the synthesis of pro-inflammatory mediators.…”

Histological and Immunohistochemical analysis of possible ameliorative effect of Ashwagandha on Methotrexate-Induced Oral Mucositis in Albino Rats

“…The anti-inflammatory effect of ASH demonstrated in our study comes in co-ordinance with Azab et al (17) who reported that ashwagandha produced a significant reduction in level of inflammatory markers (IL-17, IL-10, and α7-nAchR) and oxidative stress markers (MDA, GSH, and ROS) in spleen and liver tissues of rats treated with ASH and exposed to γ-radiation, and Elhadidy et al (30) who proved that ashwagandha extract produced anti-inflammatory and anti-oxidant effects against aluminum chloride (AlCl 3 )-induced neurotoxicity in rats, the pretreatment with ASH extract counteracted the significant rise in TNF-α, MDA and NO levels induced by AlCl 3 to nonsignificant changes as compared with the control group.…”

“…• ASH: rats were administered 300 mg/kg b.w. (17) of Ashwagandha root extract by oral gavage for 8 successive days interrupted with injection of 0.5 ml saline on day 4 of experiment.…”

Withania somnifera Alter BCL2/Bax signaling and trigger apoptosis of MCF-7 and MDA-MB231 breast cancer cells exposed to γ-radiation

“…54 In addition, it was found in our recently published research paper that Ashwagandha protect normal cells from the hazardous effect of acute and chronic radiation because of its antioxidants and anti-inflammatory capabilities. 55 However, we believed that further insightful future investigations focusing on a diverse mediator involved in autophagy and necroptosis could be with benefit to decipher this discrepancy emerged and could disclose the exact mechanism of WS.…”