Withania somnifera L.: Phenolic Compounds Composition and Biological Activity of Commercial Samples and Its Aqueous and Hydromethanolic Extracts

Połumackanycz, Milena; Petropoulos, Spyridon Α.; Śledziński, Tomasz; Goyke, Elżbieta; Konopacka, Agnieszka; Plenis, Alina; Viapiana, Agnieszka

doi:10.3390/antiox12030550

Cited by 14 publications

(10 citation statements)

References 79 publications

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“…angulata is applied as a traditional folk medicine to cure sore throats, coughs, rheumatism, and hepatitis in many countries, including China, Mexico, Indonesia, and Brazil . The fruits, a sweet or tasteless berry-type climacteric fruit, are known as wild tomato, winter cherry, and gooseberry. − A previous study on its whole plant generated a variety of withanolide derivatives, which exhibited cytotoxic and good anti-inflammatory activities. ,, UPLC-Q-Orbitrap-MS/MS was used for the first time to study bioactive withanolides from the fruits of P. angulata, As a result, many withanolide derivatives were produced with good cytotoxic and antiflammatory activities.…”

Section: Discussionmentioning

confidence: 99%

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UPLC-Q-Orbitrap-MS/MS-Guided Isolation of Bioactive Withanolides from the Fruits of Physalis angulata

Wang,

Yang,

et al. 2023

J. Agric. Food Chem.

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Physalis angulata Linn. is an exotic Amazonian fruit that is commonly recognized as wild tomato, winter cherry, and gooseberry. While its fruit is known to contain many nutrients, such as minerals, fibers, and vitamins, few papers have investigated withanolide derivatives from its fruits. UPLC-Q-Orbitrap-MS/MS, which produces fragmentation spectra, was applied for the first time to guide the isolation of bioactive withanolide derivatives from P. angulata fruits. As a result, twenty-six withanolide derivatives, including two novel 1,10-secowithanolides (1 and 2) and a new derivative (3), were obtained. Compounds 1 and 2 are rare rearranged 1,10-secowithanolides with a tetracyclic 7/6/6/5 ring system. All structures were assigned through various spectroscopic data and quantum chemical calculations. Nine withanolide derivatives exhibited significant inhibitory effects on three tumor cell lines with IC 50 values of 0.51−13.79 μM. Moreover, three new compounds (1−3) exhibited potential nitric oxide inhibitory effects in lipopolysaccharide-stimulated RAW264.7 cells (IC 50 : 7.51−61.8 μM). This investigation indicated that fruits of P. angulata could be applied to treat and prevent cancer and inflammatory-related diseases due to their potent active withanolide derivatives.

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Section: Discussionmentioning

confidence: 99%

“…Physalis angulata Linn. (Solanaceae), an annual herb, is well known as wild tomato, winter cherry, and gooseberry. − Its fruit is an exotic Amazonian fruit abundant in various nutrients, including vitamins, minerals, and fibers. In China, P.…”

Section: Introductionmentioning

confidence: 99%

UPLC-Q-Orbitrap-MS/MS-Guided Isolation of Bioactive Withanolides from the Fruits of Physalis angulata

Wang,

Yang,

et al. 2023

J. Agric. Food Chem.

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“…The positive and linear correlation of phenolic compounds as redox properties responsible for antioxidant activity has been documented in the literature. [52][53][54][55] Overall, these findings highlight the complex and diverse interactions between bioactive compounds and their impact on antioxidant and enzyme-inhibitory activities in different tissues.…”

Section: Enzyme Inhibition Effectsmentioning

confidence: 90%

The exogenous application of naringenin and rosmarinic acid modulates functional traits in Lepidium sativum

Salehi,

Zhang,

Alp‐Turgut

et al. 2023

J Sci Food Agric

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BACKGROUNDPhenolic modulators have attracted attention for their potential in shaping functional traits in plants. This work investigated the impact of naringenin (Nar) and rosmarinic acid (RA) on the functional properties of Lepidium sativum leaves and roots.ResultsUntargeted metabolomics identified a diverse phenolic profile, including flavonoids, phenolic acids, low molecular weight phenolics, lignans, and stilbenes. Cluster, analysis of variance multiblock orthogonal partial least squares (AMOPLS), and orthogonal projection to latent structures discriminant analysis (OPLS‐DA) multivariate analyses confirmed tissue‐specific modulation of bioactive compounds. The tissue was the hierarchically most influential factor, explaining 27% of observed variability, while the treatment and their interaction were statistically insignificant. Thereafter, various in vitro assays were employed to assess antioxidant capacity, including 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH), 2,2′‐azino‐bis(3‐ethylbenzothiazoline)‐6‐sulfonic acid (ABTS) radical scavenging activity, cupric ion reducing antioxidant capacity (CUPRAC), and ferric ion reducing antioxidant power (FRAP), metal chelating ability, and phosphomolybdenum (PMD) assays. Extracts were also tested for inhibitory effects on cholinesterase, amylase, glucosidase, and tyrosinase enzymes. RA application positively impacted antioxidant and enzyme inhibitory activities, holding valuable implications in shaping the health‐promoting properties of L. sativum.ConclusionThe untargeted metabolomics analysis showed a significant tissue‐dependent modulation of bioactive compounds, determining no synergistic effect between applying phenolic compounds in combination. Specifically, the sole application of RA increased anthocyanins and hydroxyphenyl propanoic acid content on leaves, which was strictly related to enhancing the biological activities. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

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“…For example, the leaves contain five unidentified alkaloids, 12 withanolides, many free amino acids, chlorogenic acid, glycosides, glucose, condensed tannins and flavonoids. In addition, the roots are rich in alkaloids, amino acids, steroids, volatile oil, starch, reducing sugars, glycosides, hentriacontane, dulcitol and withaniol [23]. It seems that this wealth of active substances is not yet fully understood and still requires further research.…”

Section: Ashwagandha's Active Substances and Their Biological Effectsmentioning

confidence: 99%

“…Classification of the active substances in W. somnifera (data from Gaurav H., 2023 [22]). Withanolides and phenolic compounds are mainly responsible for the special medicinal properties of W. somnifera and show immune system-activating and antioxidant effects, respectively [23]. Structurally, withanolides belong to the 28-carbon atoms of natural steroidal lactones, built on an ergostane skeleton in which C-22 and C-26 are oxidized to form six-or five-membered lactone rings.…”

Section: Ashwagandha's Active Substances and Their Biological Effectsmentioning

confidence: 99%

Can Ashwagandha Benefit the Endocrine System?—A Review

Wiciński,

Fajkiel-Madajczyk,

Kurant

et al. 2023

IJMS

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Withania somnifera, also known as Ashwagandha, has been used in traditional medicine for thousands of years. Due to the wide range of its activities, there has been interest in its possible beneficial effects on the human body. It is proved that, among others, Ashwagandha has anti-stress, anti-inflammatory, antimicrobial, anti-cancer, anti-diabetic, anti-obesity, cardioprotective, and hypolipidemic properties. Particularly interesting are its properties reported in the field of psychiatry and neurology: in Alzheimer’s disease, Parkinson’s disease, multiple sclerosis, depression, bipolar disorder, insomnia, anxiety disorders and many others. The aim of this review is to find and summarize the effect that Ashwagandha root extract has on the endocrine system and hormones. The multitude of active substances and the wide hormonal problems faced by modern society sparked our interest in the topic of Ashwagandha’s impact on this system. In this work, we also attempted to draw conclusions as to whether W. somnifera can help normalize the functions of the human endocrine system in the future. The search mainly included research published in the years 2010–2023. The results of the research show that Ashwagandha can have a positive effect on the functioning of the endocrine system, including improving the secretory function of the thyroid gland, normalizing adrenal activity, and multidirectional improvement on functioning of the reproductive system. The main mechanism of action in the latter appears to be based on the hypothalamus–pituitary–adrenal (HPA) axis, as a decrease in cortisol levels and an increase in hormones such as luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in men were found, which results in stress level reduction and improvement in fertility. In turn, other studies prove that active substances from W. somnifera, acting on the body, cause an increase in the secretion of triiodothyronine (T3) and thyroxine (T4) by the thyroid gland and a subsequent decrease in the level of thyroid-stimulating hormone (TSH) in accordance with the hypothalamus–pituitary–thyroid (HPT) axis. In light of these findings, it is clear that Ashwagandha holds significant promise as a natural remedy for various health concerns, especially those related to the endocrine system. Future research may provide new insights into its mechanisms of action and expand its applications in both traditional and modern medicine. The safety and toxicity of Ashwagandha also remain important issues, which may affect its potential use in specific patient groups.

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Withania somnifera L.: Phenolic Compounds Composition and Biological Activity of Commercial Samples and Its Aqueous and Hydromethanolic Extracts

Cited by 14 publications

References 79 publications

UPLC-Q-Orbitrap-MS/MS-Guided Isolation of Bioactive Withanolides from the Fruits of Physalis angulata

UPLC-Q-Orbitrap-MS/MS-Guided Isolation of Bioactive Withanolides from the Fruits of Physalis angulata

The exogenous application of naringenin and rosmarinic acid modulates functional traits in Lepidium sativum

Can Ashwagandha Benefit the Endocrine System?—A Review

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