Withdrawal of Continuous Positive Airway Pressure Therapy for 2 Weeks in Obstructive Sleep Apnoea Patients Results in Increased Circulating Platelet and Leucocyte-Derived Microvesicles

Ayers, Lisa; Turnbull, Chris D.; Petousi, Nayia; Ferry, Berne; Kohler, Malcolm; Stradling, John

doi:10.1159/000446077

Cited by 4 publications

(5 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In more severe cases of OSA, PMP plasma levels correlate with AHI ( 85 ). Conversely, withdrawal of CPAP therapy resulted in increase of PMPs ( 86 ).…”

Section: Platelets In Osamentioning

confidence: 99%

Obstructive Sleep Apnea: From Intermittent Hypoxia to Cardiovascular Complications via Blood Platelets

et al. 2018

View full text Add to dashboard Cite

Obstructive sleep apnea is a chronic condition characterized by recurrent episodes of apneas or hypopneas during sleep leading to intermittent hypoxemia and arousals. The prevalence of the sleep disordered breathing is estimated that almost 50% of men and 24% of women suffer from moderate to severe form of the disorder. Snoring, collapse of upper airways and intermittent hypoxia are main causes of smoldering systemic inflammation in patients suffering from obstructive sleep apnea. The systematic inflammation is considered one of the key mechanisms leading to significant cardiovascular complications. Blood platelets, formerly not even recognized as cells, are currently gaining attention as crucial players in the immune continuum. Platelet surface is endowed with receptors characteristic for cells classically belonging to the immune system, which enables them to recognize pathogens, immune complexes, and interact in a homo- and heterotypic aggregates. Platelets participate in the process of transcellular production of bioactive lipids by delivering both specific enzymes and substrate molecules. Despite their lack of nucleus, platelets synthetize proteins in a stimuli-dependent manner. Atherosclerosis and consequent cardiovascular complications result from disruption in homeostasis of both of the platelet roles: blood coagulation and inflammatory processes modulation. Platelet parameters, routinely evaluated as a part of complete blood count test, were proposed as markers of cardiovascular comorbidity in patients with obstructive sleep apnea. Platelets were found to be excessively activated in this group of patients, especially in obese subjects. Persistent activation results in enhanced spontaneous aggregability and change in cytokine production. Platelet-lymphocyte ratio was suggested as an independent marker for cardiovascular disease in obstructive sleep apnea syndrome and continuous positive air pressure therapy was found to have an impact on platelet parameters and phenotype. In this literature review we summarize the current knowledge on the subject of platelets involvement in obstructive sleep apnea syndrome and consider the possible pathways in which they contribute to cardiovascular comorbidity.

show abstract

“…In more severe cases of OSA, PMP plasma levels correlate with AHI ( 85 ). Conversely, withdrawal of CPAP therapy resulted in increase of PMPs ( 86 ).…”

Section: Platelets In Osamentioning

confidence: 99%

Obstructive Sleep Apnea: From Intermittent Hypoxia to Cardiovascular Complications via Blood Platelets

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Limitations in study design that do not allow for causative associations to be established between OSA and changes in coagulation or that do not adequately control for comorbidities or OSA severity or phenotypes are a common problem. However, there are some data from RCTs and meta-analyses that strengthen the level of evidence, e.g., on increased levels of procoagulant and platelet-derived microvesicles or fibrinogen in OSA [166,172].…”

Section: Discussion Of Major Findingsmentioning

confidence: 98%

“…Significantly decreased levels of vWF were detected 6 months after upper airway surgery [165]. Two weeks of CPAP withdrawal resulted in an elevation in the levels of PMPs [166]; however, the same workgroup also reported conflicting results [167]. Higher body mass index and disease severity of participants in the former study may explain the different results.…”

Section: The Effect Of Osa Treatment On Coagulation Fibrinolysis Anmentioning

confidence: 92%

Coagulation and Fibrinolysis in Obstructive Sleep Apnoea

Bikov

Mészáros

Schwarz

2021

IJMS

View full text Add to dashboard Cite

Obstructive sleep apnoea (OSA) is a common disease which is characterised by repetitive collapse of the upper airways during sleep resulting in chronic intermittent hypoxaemia and frequent microarousals, consequently leading to sympathetic overflow, enhanced oxidative stress, systemic inflammation, and metabolic disturbances. OSA is associated with increased risk for cardiovascular morbidity and mortality, and accelerated coagulation, platelet activation, and impaired fibrinolysis serve the link between OSA and cardiovascular disease. In this article we briefly describe physiological coagulation and fibrinolysis focusing on processes which could be altered in OSA. Then, we discuss how OSA-associated disturbances, such as hypoxaemia, sympathetic system activation, and systemic inflammation, affect these processes. Finally, we critically review the literature on OSA-related changes in markers of coagulation and fibrinolysis, discuss potential reasons for discrepancies, and comment on the clinical implications and future research needs.

show abstract

“…A number of studies have highlighted the potential contributions of EVs to human diseases, and OSA is not an exception. Studies on the involvement of EVs in the emergence of endothelial cell dysfunction and adipocyte insulin resistance in both patients suffering from OSA and animal models of OSA have provided robust initial evidence supporting such critical roles [ 9 , 21 , 28 , 55 , 64 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 ]. EV cargo is altered in response to hypoxia.…”

Section: Discussionmentioning

confidence: 99%

Cell-Selective Altered Cargo Properties of Extracellular Vesicles Following In Vitro Exposures to Intermittent Hypoxia

Sanz-Rubio

Qiao

Ullate

et al. 2021

IJMS

View full text Add to dashboard Cite

Intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), is associated with cardiovascular and metabolic dysfunction. However, the mechanisms underlying these morbidities remain poorly delineated. Extracellular vesicles (EVs) mediate intercellular communications, play pivotal roles in a multitude of physiological and pathological processes, and could mediate IH-induced cellular effects. Here, the effects of IH on human primary cells and the release of EVs were examined. Microvascular endothelial cells (HMVEC-d), THP1 monocytes, THP1 macrophages M0, THP1 macrophages M1, THP1 macrophages M2, pre-adipocytes, and differentiated adipocytes (HAd) were exposed to either room air (RA) or IH for 24 h. Secreted EVs were isolated and characterized using transmission electron microscopy, nanoparticle tracking analysis, and Western blotting. The effects of each of the cell-derived EVs on endothelial cell (EC) monolayer barrier integrity, on naïve THP1 macrophage polarity, and on adipocyte insulin sensitivity were also evaluated. IH did not alter EVs cell quantal release, but IH-EVs derived from HMVEC-d (p < 0.01), THP1 M0 (p < 0.01) and HAd (p < 0.05) significantly disrupted HMVEC-d monolayer integrity, particularly after H2O2 pre-conditioning. IH-EVs from HMVEC-d and THP1 M0 elicited M2-polarity changes did not alter insulin sensitivity responses. IH induces cell-selective changes in EVs cargo, which primarily seem to target the emergence of endothelial dysfunction. Thus, changes in EVs cargo from selected cell sources in vivo may play causal roles in some of the adverse outcomes associated with OSA.

show abstract

Withdrawal of Continuous Positive Airway Pressure Therapy for 2 Weeks in Obstructive Sleep Apnoea Patients Results in Increased Circulating Platelet and Leucocyte-Derived Microvesicles

Cited by 4 publications

References 13 publications

Obstructive Sleep Apnea: From Intermittent Hypoxia to Cardiovascular Complications via Blood Platelets

Obstructive Sleep Apnea: From Intermittent Hypoxia to Cardiovascular Complications via Blood Platelets

Coagulation and Fibrinolysis in Obstructive Sleep Apnoea

Cell-Selective Altered Cargo Properties of Extracellular Vesicles Following In Vitro Exposures to Intermittent Hypoxia

Contact Info

Product

Resources

About