Withdrawal: Salvianolic Acid B Protects SH-SY5Y Neuroblastoma Cells from 1-Methyl-4-phenylpyridinium-Induced Apoptosis

Zeng, Guang; Tang, Tao; Wu, Hong; You, Wei; Luo, Jie-Kun; Lin, Yuan; Lu, Qinghua; Li, Xing-Qun; Huang, Xi; Yang, Qidong

doi:10.1248/bpb.33.1337

Cited by 36 publications

(28 citation statements)

References 31 publications

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“…The present study observed that salvianolic acid B suppressed cell proliferation, induced apoptosis and activated cleaved caspase 3 of the osteosarcoma MG63 cell. Wang et al (12) suggested that salvianolic acid B induced apoptosis through p38-mediated ROS generation in human glioma U87 cells and suppressed the growth and angiogenic potential of oral squamous carcinoma cells (20) and neuroblastoma SH-SY5Y cell lines (16).…”

Section: Discussionmentioning

confidence: 99%

“…1, is a low-polymer compound with a relative molecular weight of 718.59 and comprises 1 molecule of caffeic acid and 3 molecules of danshensu, with a molecular formula of C 36 H 36 0 16 (15). Salvianolic acid B possess anti-oxidative and anti-inflammation effects (16). At present, salvianolic acid B has been demonstrated to exhibit the strongest pharmacological activity in Danshen water-soluble substances (14).…”

Section: Introductionmentioning

confidence: 99%

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Salvianolic acid B suppresses cell proliferation and induces apoptosis in osteosarcoma through p38-mediated reactive oxygen species generation

et al. 2017

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Abstract. The present study aimed to investigate the potential anticancer effect and mechanisms of salvianolic acid B on osteosarcoma. Salvianolic acid B suppressed osteosarcoma cell proliferation and induced apoptosis in the osteosarcoma MG63 cell line, and activated the expressions of cleaved caspase-3, phosphorylated-tumor protein (p)38 mitogen-activated protein kinase (p-p38 MAPK) and phosphorylated-p53 (p-p53) proteins in the MG63 cells. Additionally, Salvianolic acid B also increased the level of reactive oxygen species (ROS) generation in the MG63 cells. The silencing of p38 expression inhibited the anticancer effect of salvianolic acid B on the levels of cell proliferation, p-p53 protein expression and ROS generation level in the MG63 cells. All these data supported the hypothesis that the anticancer effect of salvianolic acid B includes the suppression of cell proliferation and induces apoptosis in MG63 cells, and that p38 is important in the anticancer effect of salvianolic acid B on osteosarcoma cells due to the direct regulation of ROS generation. These data suggest that salvianolic acid B is important in the proliferation of osteosarcoma cells due to the direct regulation of p38-mediated ROS signaling. IntroductionOsteosarcoma is a type of primary malignant tumor that exhibits the highest morbidity of all neoplasms in the human skeletal system, and often presents in the metaphysis of the long tubal bones (1). Osteosarcoma often affects young people between the age of 20 and 30 years old. The mortality rate is high (2) and ~20% of patients exhibit pulmonary metastasis prior to diagnosis. Subsequent to diagnosis, the majority of patients succumb to the disease within 2 years (2). At present, there are no effective therapeutic treatments for early osteosarcoma (3). Therefore, it is important to investigate the causes of osteosarcoma occurrence, development and invasion, the mechanisms of osteosarcoma oncogenesis, and to identify effective diagnostic and therapeutic techniques. The development of gene therapy has created novel research targets in oncotherapy, including the identification of a target gene (4).Mitogen-activated protein kinase (MAPK) is the one of the most important types of signal conduction pathway in humans, and interacts with multiple signaling pathways (5). The tumor protein (p)38 MAPK pathway is activated through phosphotyrosine and threonine and inflammatory and growth factors, and activates downstream transcription factors on target genes, increases the initiation of cancer cell development, promotes protein synthesis, regulates cell surface receptors and regulates the invasion and transfer of tumor cells (6).Reactive oxygen species (ROS) are secondary products in the process of aerobic metabolism and include oxygen ions, peroxide and oxygen radical molecules (7). The increase in the level of intracellular ROS may promote cellular proliferation and differentiation to some extent. However, excessive levels of ROS results in damage to lipids, proteins and DNA, destroying numerous nor...

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Salvianolic acid B suppresses cell proliferation and induces apoptosis in osteosarcoma through p38-mediated reactive oxygen species generation

et al. 2017

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“…Moreover, ROS trigger caspase-3 and caspase-3-like proteases in different cell categories including neuronal cells, resulting in nuclear compression and DNA disintegration [83,[86][87][88][89]. Nevertheless, ROS also trigger apoptosis, possibly by reducing secretion of Bcl-2, an antiapoptotic molecule [83,90,91]. Also, Bcl-2 and Bax may regulator the mitochondrial penetrability modification pore, which can instigate the movement of cytochrome c and some apoptosis-triggered dynamics that activate the stimulation of caspase cascade leading to apoptosis [83,90].…”

Section: α-Mg and Apoptosismentioning

confidence: 99%

“…Nevertheless, ROS also trigger apoptosis, possibly by reducing secretion of Bcl-2, an antiapoptotic molecule [83,90,91]. Also, Bcl-2 and Bax may regulator the mitochondrial penetrability modification pore, which can instigate the movement of cytochrome c and some apoptosis-triggered dynamics that activate the stimulation of caspase cascade leading to apoptosis [83,90]. Studies [83,92] have proven that caspase-3 is associated with apoptotic activities happening in the mitochondrial-adjunct pathway, which are related to nuclear compression and DNA cleavage.…”

Section: α-Mg and Apoptosismentioning

confidence: 99%

The Pivotal Neuroinflammatory, Therapeutic and Neuroprotective Role of Alpha-Mangostin

Richard

Zheng

et al. 2017

J Neurol Res

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Alpha-mangostin (α-MG), one of the key xanthone derivatives, displays a multiplicity of curative qualities like antiinfective, anti-malarial, anticarcinogenic, antidiabetic actions as well as antioxidant properties. Furthermore, it has proven to have neuroprotective, hepatoprotective, and cardioprotective features, of which the anticarcinogenic action is the most auspicious. Additionally, several in vitro and in vivo analyses confirm that α-MG partakes in the major stages of tumor growth: initiation, promotion, and progression. Nevertheless, α-MG services as an inhibitory agent that modulate various enzymes involved in the metabolic activation and excretion of carcinogens, resistance to oxidative damage, and attenuation of inflammatory response. Numerous studies have also implicated α-MG in central nervous system (CNS) disorders, among which neuroinflammatory disorders and brain cancer are cardinal. Based on these initial studies on α-MG, we reviewed the pivotal role of α-MG in neuroinflammatory disorders.

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“…Recently, MLB was suggested to be responsible for antiischemic neuroprotection by its effective inhibition of the Na + /K + -ATPase (Tzen et al, 2007). The ability of MLB to cross the blood-brain barrier (BBB) (Liu et al, 2006) and protect SH-SY5Y cells against MPP(+)-induced apoptosis by relieving oxidative stress and modulating the apoptotic process of neural stem cells (Zeng et al, 2010) has also been shown. Notably, MLB could ameliorate learning and memory dysfunctions induced by cerebral transient ischemia (Du et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Antidepressant-like effects of magnesium lithospermate B in a rat model of chronic unpredictable stress

Quan

Liu

Zhang

et al. 2015

Pharmaceutical Biology

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Materials and methods: Sprague-Dawley rats were subjected to the chronic unpredictable stress (CUS) protocol. Rats in the control group received no CUS during the whole experiment. In the model group, rats were exposed to CUS for 7 weeks. From the beginning of the 5th week, model group rats were randomly grouped and subjected to different treatments. In the experiment, control and model group rats were intraperitoneally (i.p.) injected with saline. MLB was dissolved in saline to give a final concentration, and the rats were injected (i.p.) with 15, 30, or 60 mg/kg MLB once a day for 3 weeks. Results: MLB administration significantly reduced: (1) the immobility time in the forced swimming test (19 s, p50.05); (2) the immobility time in the tail suspension test (76.3 s, p50.05); (3) the corticosterone (CORT) concentrations in the serum (21.7 nmol/L, p40.05); (4) the pro-inflammatory cytokine levels in the serum -TNF-a (92.1 pg/ml, p50.05), IL-1b (86.9 pg/ ml, p50.05), and IL-6 (93.8 pg/ml, p50.05); (5) pro-inflammatory cytokine levels in tissue -TNF-a (3.2 pg/mg protein, p50.05), IL-1b (1.5 pg/mg protein, p40.05), and IL-6 (6.3 pg/mg protein, p50.05); and (6) phospho-NF-kB (1.6, p50.05) and phospho-IkB-a (0.4, p50.05) expression in tissue. Discussion and conclusion: The results suggested that MLB might exert therapeutic actions on depression-like behavior and the HPA axis hyperactivity in CUS rats, and the mechanisms underlying the antidepressant-like effects of MLB might be mediated by regulation of the expression of NF-kB and IkB-a in rats.

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Withdrawal: Salvianolic Acid B Protects SH-SY5Y Neuroblastoma Cells from 1-Methyl-4-phenylpyridinium-Induced Apoptosis

Cited by 36 publications

References 31 publications

Salvianolic acid B suppresses cell proliferation and induces apoptosis in osteosarcoma through p38-mediated reactive oxygen species generation

Salvianolic acid B suppresses cell proliferation and induces apoptosis in osteosarcoma through p38-mediated reactive oxygen species generation

The Pivotal Neuroinflammatory, Therapeutic and Neuroprotective Role of Alpha-Mangostin

Antidepressant-like effects of magnesium lithospermate B in a rat model of chronic unpredictable stress

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