WITHDRAWN: Epigenetic silence of HOTAIR contributes to the metastasis of pancreatic cancer via targeting miR-138

Nie, Hanqiu; Zhang, Yang; Xing, Renwei; Li, Minxian; Mou, Yi

doi:10.3727/096504017x15138544374391

Cited by 3 publications

(4 citation statements)

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“…In breast and pancreatic cancer, HOTAIR has been shown to be aberrantly overexpressed and regulates cell invasion [12,13]. Moreover, HOTAIR has been shown to play a role in metastasis of oral and pancreatic cancer [14,15]. In yet another study knockdown of HOTAIR in breast cancer has been shown to be accompanied by suppression of cell migration [16].…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA HOTAIR regulates proliferation, migration and invasion of human cervical cancer cells by modulating expression of MAPK1

Liu

Zhao

2020

aoms

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Introduction: Accumulating evidence suggests that long non-coding RNAs (lncRNAs) are dysregulated in cancer cells and may be responsible for the development and progression of this disease. Herein, the role and therapeutic potential of aberrantly expressed lncRNA HOTAIR were investigated in cervical cancer. Material and methods: The expression profile of the lncRNA HOTAIR was determined by quantitative RT-PCR. CCK-8 and colony formation assays were used for determination of cell viability. DAPI and annexin V/PI assays were used for detection of apoptosis. Wound healing and transwell assays were used to monitor cell migration and invasion. Results: The results showed that the expression of lncRNA HOTAIR was significantly (p < 0.01) upregulated (up to 4.1-fold) in cervical cancer cell lines. Silencing of lncRNA HOTAIR expression resulted in inhibition of the proliferation of the DoTc2 cervical cancer cells via induction of apoptotic cell death. HOTAIR silencing also resulted in decrease of the migration and the invasive properties of the cervical cancer cells. HOTAIR has been reported to interact with MAPK1 in cancer cells, and in this study MAPK1 was found to be overexpressed (up to 3.7-fold) in all the cervical cancer cells and silencing of HOTAIR inhibited the expression of MAPK1 in DoTc2 cervical cancer cells. Silencing of MAPK1 in DoTc2 cells also inhibited their proliferation and metastasis via induction of apoptosis. Co-transfection experiments showed that silencing of MAPK1 and lncRNA HOTAIR causes inhibition of DoTc2 cell growth synergistically. Conclusions: These results indicate that lncRNA HOTAIR may prove to be an important therapeutic target for management of cervical cancer.

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Section: Introductionmentioning

confidence: 99%

Long non-coding RNA HOTAIR regulates proliferation, migration and invasion of human cervical cancer cells by modulating expression of MAPK1

Liu

Zhao

2020

aoms

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“…In a recent study, Jiang et al (15) reported that lncRNA HOTAIR was upregulated in pancreatic ductal adenocarcinoma tissue samples compared with adjacent healthy tissues. However, in another study, epigenetic silencing of HOTAIR was proved to be at least partially responsible for the metastasis of pancreatic cancer (16). The present results revealed that the expression of HOTAIR was significantly upregulated in tumor tissues compared with adjacent healthy tissues in 46 out of 51 patients, whereas downregulation was observed in only 2 cases.…”

Section: Discussionmentioning

confidence: 99%

“…These data suggest that HOTAIR and HK2 may serve as diagnostic and prognostic biomarkers of pancreatic adenocarcinoma. However, HOTAIR and HK2 have also been reported to be of diagnostic and prognostic value in numerous other types of malignancy, inevitably affecting their specificity (15,16,18). Therefore, the combination of multiple biomarkers may improve the diagnosis and prognosis of pancreatic adenocarcinoma.…”

Section: Discussionmentioning

confidence: 99%

“…In the present study, compared with their equivalent controls, HOTAIR and HK2 overexpression significantly promoted the proliferation of the two tested pancreatic adenocarcinoma cell lines, BxPC-3 and Capan-2. Despite the fact that the function of HOTAIR has been studied in various types of cancer, including pancreatic cancer (15,16), its involvement in cancer cell energy metabolism, which is critical for cell growth and proliferation, remains unclear. Until now, a number of lncRNAs have been demonstrated to participate in glucose metabolism in cancer (20), and Cao et al (21) proposed an electron-transfer equilibrium with a small reorganization energy in aerobic glycolysis by using synthetic model systems.…”

Section: Discussionmentioning

confidence: 99%

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Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2

Zhou

et al. 2019

Oncol Lett

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Hox transcript antisense RNA (HOTAIR) is a long non-coding RNA (lncRNA) that serves a key role in the pathogenesis of various types of cancer, including pancreatic adenocarcinoma. However, the diagnostic and prognostic values of HOTAIR in pancreatic adenocarcinoma, as well as its involvement in cancer cell energy metabolism, remain unclear. In the present study, tumor tissues and adjacent healthy tissues were collected from patients with pancreatic adenocarcinoma, and blood samples were collected from patients and healthy controls. Expression levels of HOTAIR and hexokinase-2 (HK2) were detected by reverse transcription-quantitative polymerase chain reaction. All patients were followed up for 5 years, and the diagnostic and prognostic values of serum HOTAIR levels were investigated by receiver operating characteristic curve and survival analyses, respectively. Pancreatic adenocarcinoma cell lines overexpressing HOTAIR and HK2 were established, and the effects on cell proliferation, lactate production, glucose uptake and ATP production were detected by Cell Counting Kit-8, lactate, glucose uptake and ATP assays, respectively. The protein expression was detected by western blot analysis. The results revealed that HOTAIR and HK2 expression levels were increased in tumor tissues compared with adjacent healthy tissues. The serum levels of HOTAIR and HK2 were higher in patients with pancreatic cancer compared with healthy controls. The serum levels of these two factors may be used to accurately predict pancreatic adenocarcinoma and its prognosis. HOTAIR and HK2 overexpression led to the promotion of tumor cell proliferation. HOTAIR overexpression increased lactate production, glucose uptake and ATP production. Furthermore, it promoted HK2 expression, however HK2 overexpression displayed no significant effects on HOTAIR expression levels. Therefore, it was concluded that the lncRNA HOTAIR may promote cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating HK2.

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Long non-coding RNA HOTAIR and STAT3 synergistically regulate the cervical cancer cell migration and invasion

Zhang

Cheng

Liu

et al. 2018

Chemico-Biological Interactions

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WITHDRAWN: Epigenetic silence of HOTAIR contributes to the metastasis of pancreatic cancer via targeting miR-138

Abstract: Ahead of Print article withdrawn by publisher.

Cited by 3 publications

References 0 publications

Long non-coding RNA HOTAIR regulates proliferation, migration and invasion of human cervical cancer cells by modulating expression of MAPK1

Long non-coding RNA HOTAIR regulates proliferation, migration and invasion of human cervical cancer cells by modulating expression of MAPK1

Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2

Long non-coding RNA HOTAIR and STAT3 synergistically regulate the cervical cancer cell migration and invasion

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