WITHDRAWN: Identification and Validation of Autophagy-related Genes in Sepsis-related Acute Kidney Injury (S-AKI) via Bioinformatics Analysis

Wang, Yuting; Bi, Xinwen; Sheng, Yu; Zeng, Ling; Zheng, Xiaoming; Chi, Xinjin

doi:10.2174/1386207326666230331075912

Cited by 2 publications

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“…Two additional studies published recently also indicated that MAP1LC3B may influence the development of sepsis and sepsis-related acute kidney injury. [36,37] Therefore, based on previous reports and the results of our study, we hypothesize that MAP1LC3B might have an important role in stimulating immune infiltration in blood tissues and thus contributes to the progression of septic shock. Currently, the specific relationships between MAP1LC3B and septic shock have not been revealed; thus, our findings provide reliable evidence for the correlation between the 2 and validate the fundamental roles of MAP1LC3B in septic shock-related immune infiltration.…”

Section: Discussionsupporting

confidence: 61%

Mitophagy-related genes could facilitate the development of septic shock during immune infiltration

Yang,

Zheng,

Liu

et al. 2023

Medicine

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Septic shock often occurs following critically low blood pressure in patients with sepsis, and is accompanied by a high death rate. Although mitophagy is associated with infection and immune responses, its role in septic shock remains unknown. This study screened effective mitophagy-related genes (MRGs) for medical practice and depicted immune infiltration situations in patients with septic shock. Gene expression profiles of GSE131761 from the Gene Expression Omnibus database were compiled for differential analysis, weighted gene co-expression network analysis, and immune infiltration analysis, while other GSE series were used as validation datasets. A series of validation methods were used to verify the robustness of hub genes, while a nomogram and prognosis model were established for medical practice. Six genes were screened via combinations of differentially expressed genes, weighted gene co-expression network analysis, and MRGs. From this, 3 hub genes (MAP1LC3B, ULK1, and CDC37) were chosen for subsequent analysis based on different validation methods. Gene set enrichment analysis showed that leukocyte trans-endothelial migration and the p53 signaling pathway were abnormally activated during septic shock. Immune infiltration analysis indicated that the imbalance of neutrophils and CD4 naive T cells was significantly correlated with septic shock progression. A nomogram was generated based on MAP1LC3B, ULK1, and CDC37, as well as age. The stability of our model was confirmed using a calibration plot. Importantly, patients with septic shock with the 3 highly expressed hub genes displayed worse prognosis than did patients without septic shock. MAP1LC3B, ULK1, and CDC37 are considered hub MRGs in the development of septic shock and could represent promising diagnostic and prognostic biomarkers in blood tissue. The validated hub genes and immune infiltration pattern expand our knowledge on MRG functional mechanisms, which provides guidance and direction for the development of septic shock diagnostic and therapeutic markers.

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Section: Discussionsupporting

confidence: 61%

Mitophagy-related genes could facilitate the development of septic shock during immune infiltration

Yang,

Zheng,

Liu

et al. 2023

Medicine

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The impact of ulinastatin on lymphocyte apoptosis and autophagy in sepsis patients

Zhang,

Song,

Zhan

et al. 2024

Sci Rep

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This study aimed to assess the influence of ulinastatin (UTI) on lymphocyte apoptosis and autophagy in sepsis patients, as well as its effect on inflammatory factors and vital organ function, with the goal of providing insights for improved clinical management of sepsis. A total of 40 sepsis patients were randomly assigned to the UTI group or the control group. The UTI group received standard treatment plus intravenous UTI, while the control group received standard treatment alone. Peripheral blood samples were collected at multiple time points for analysis of lymphocyte apoptosis, autophagy, inflammatory markers, and organ function. Various experimental techniques including Hoechst staining, transmission electron microscopy, and Western blot analysis were utilized to assess lymphocyte apoptosis, autophagy, and related protein expression levels. The study revealed that UTI treatment significantly inhibited lymphocyte apoptosis and promoted autophagy in sepsis patients. The levels of autophagy-related proteins LC3-II and Beclin-1 were substantially elevated, while the ratio of anti-apoptotic Bcl-2 to pro-apoptotic Bax was increased following UTI treatment. Furthermore, the levels of inflammatory markers IL-6, procalcitonin, and C-reactive protein were markedly reduced in the UTI group compared to the control group. Additionally, UTI treatment led to improved liver, kidney and cardiac function as evidenced by reduced levels of liver enzymes and creatinine, and cardiac enzymes. The findings of this study demonstrate that UTI exerts a protective effect on septic patients by inhibiting lymphocyte apoptosis, promoting autophagy, and attenuating systemic inflammation. Moreover, UTI treatment was associated with improved liver, kidney, and cardiac function in septic patients. These results contribute to a better understanding of the clinical management of sepsis and underscore the potential of UTI as a therapeutic intervention in septic patients. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-79878-y.

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WITHDRAWN: Identification and Validation of Autophagy-related Genes in Sepsis-related Acute Kidney Injury (S-AKI) via Bioinformatics Analysis

Cited by 2 publications

References 0 publications

Mitophagy-related genes could facilitate the development of septic shock during immune infiltration

Mitophagy-related genes could facilitate the development of septic shock during immune infiltration

The impact of ulinastatin on lymphocyte apoptosis and autophagy in sepsis patients

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