[WITHDRAWN] miR-144-3p Functions as a Tumor Suppressor in Endometrial Cancer by Targeting PRR11

Song, Hwangjun; Sun, Han‐Dong; Pang, Xuecheng; Qian, Sumin; Zhang, Xiang; Huang, Yue; Liu, Xueliang

doi:10.1016/j.amjms.2022.02.011

“…HOXA9 gene was a cluster A HOX gene located on chromosome 7. Most studies have shown that the progression of multiple malignancies was associated with aberrant HOXA9 expression [ 32 ]. The HOXA9 mRNA level in colorectal cancer tissues and cells were detected by qRT-PCR, and the results were similar to the study by Bhatlekar et al: HOXA9 is highly expressed in CRC, which revealed that HOXA9 promotes CRC progression [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis

Huang

¹

,

Bai

²

,

Wang

³

et al. 2022

Analytical Cellular Pathology

4

0

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Background. The mortality rate of colorectal cancer (CRC) ranks second. circRNAs are abnormal expression in some diseases, and their dysregulation is associated with cancer progression. Recent studies have shown that the malignant progression of colorectal cancer is inseparable from the abnormal expression of circRNAs. Methods. First, the circ_0052184 expression in clinical tissue and cell samples was analyzed by qRT-PCR. Then, we constructed circ_0052184-silenced CRC cells and detected by qRT-PCR. Furthermore, the proliferation ability of cells was detected by colony formation assay. Cell migration ability was tested by wound healing assay and transwell assay. Cell invasion ability was detected by transwell assay. Results. Expression of circ_0052184 was significantly increased in colorectal cancer cell lines and tissues. Silencing circ_0052184 affected the proliferation, migration, and invasion of colorectal cancer cells. miR-604 was targeted by circ_0052184. The downstream target of miR-604 was HOXA9, and silencing circ_0052184 inhibited HOXA9 expression. The existence of the circ_0052184/miR-604/HOXA9 regulatory network in colorectal cancer was validated. circ_0052184 promoted the occurrence and development of colorectal cancer by targeting the miR-604/HOXA9 axis. Conclusions. Our study revealed that the molecular mechanism of circ_0052184 regulated the miR-604/HOXA9 axis, which might promote the malignant progression of colorectal cancer cells.

show abstract

circ_0052184 promotes colorectal cancer progression via targeting miR-604/HOXA9 axis

Huang

¹

,

Bai

²

,

Wang

³

et al. 2022

Preprint

1

0

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Background:The mortality rate of colorectal cancer (CRC) ranks second. CircRNAs are abnormal expression in some diseases, and their dysregulation is associated with cancer progression. Recent studies have shown that the malignant progression of colorectal cancer is inseparable from the abnormal expression of circRNAs.Methods:First, the circ_0052184 expression in clinical tissue and cell samples was analyzed by qRT-PCR. Then, we constructed circ_0052184-silenced CRC cells and detected by qRT-PCR. Furthermore, the proliferation ability of cells was detected by colony formation assay. Cell migration ability was tested by wound healing assay and transwell assay. Cell invasion ability were detected by transwell assay.Results:Expression of circ_0052184 was significantly increased in colorectal cancer cell lines and tissues. Silencing circ_0052184 affected the proliferation, migration and invasion of colorectal cancer cells. MiR-604 was targeted by circ_0052184. The downstream target of miR-604 was HOXA9, and silencing circ_0052184 inhibited HOXA9 expression. The existence of circ_0052184/miR-604/HOXA9 regulatory network in colorectal cancer was validated. Circ_0052184 promoted the occurrence and development of colorectal cancer by targeting the miR-604/HOXA9 axis.Conclusions:Our study revealed that the molecular mechanism of circ_0052184 regulated the miR-604/HOXA9 axis, which might promote the malignant progression of colorectal cancer cells.

show abstract

circ_0052184 promotes colorectal cancer progression via targeting miR-604/HOXA9 axis

Huang

¹

,

Bai

²

,

Wang

³

et al. 2022

Preprint

0

View full text Add to dashboard Cite

Background:The mortality rate of colorectal cancer (CRC) ranks second. CircRNAs are abnormal expression in some diseases, and their dysregulation is associated with cancer progression. Recent studies have shown that the malignant progression of colorectal cancer is inseparable from the abnormal expression of circRNAs.Methods:First, the circ_0052184 expression in clinical tissue and cell samples was analyzed by qRT-PCR. Then, we constructed circ_0052184-silenced CRC cells and detected by qRT-PCR. Furthermore, the proliferation ability of cells was detected by colony formation assay. Cell migration ability was tested by wound healing assay and transwell assay. Cell invasion ability were detected by transwell assay.Results:Expression of circ_0052184 was significantly increased in colorectal cancer cell lines and tissues. Silencing circ_0052184 affected the proliferation, migration and invasion of colorectal cancer cells. MiR-604 was targeted by circ_0052184. The downstream target of miR-604 was HOXA9, and silencing circ_0052184 inhibited HOXA9 expression. The existence of circ_0052184/miR-604/HOXA9 regulatory network in colorectal cancer was validated. Circ_0052184 promoted the occurrence and development of colorectal cancer by targeting the miR-604/HOXA9 axis.Conclusions:Our study revealed that the molecular mechanism of circ_0052184 regulated the miR-604/HOXA9 axis, which might promote the malignant progression of colorectal cancer cells.

show abstract

[WITHDRAWN] miR-144-3p Functions as a Tumor Suppressor in Endometrial Cancer by Targeting PRR11

Cited by 3 publications

References 32 publications

circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis

circ_0052184 Promotes Colorectal Cancer Progression via Targeting miR-604/HOXA9 Axis

circ_0052184 promotes colorectal cancer progression via targeting miR-604/HOXA9 axis

circ_0052184 promotes colorectal cancer progression via targeting miR-604/HOXA9 axis

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