Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm

Waterhouse, Karin Elisabeth; Hofmo, P. O.; Tverdal, Aage; Miller, Robert C.

doi:10.1530/rep.1.01049

Cited by 161 publications

(121 citation statements)

References 30 publications

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“…The basic mechanisms of individual genetic differences connected with the susceptibility to damage induced by cryopreservation remain unknown, but they may be manifested in the varied biochemical composition of semen (Holt et al 2005). For example, Waterhouse et al (2006) stated that differences in individual sensitivity of boar spermatozoa to cryopreservation are connected with the amount of long-chain polyunsaturated fatty acids (PUFAs) contained in plasma membranes. Since boar spermatozoa are characterized by a high level of long-chain polyunsaturated fatty acids (PUFAs) and a low cholesterol level in membranes, they are particularly sensitive to cooling processes (Park andLynch 1992, Penny et al 2000).…”

Section: Discussionmentioning

confidence: 99%

Boar variability affects sperm metabolism activity in liquid stored semen at 5°C

Dziekońska¹,

Strzeżek²

2011

Polish Journal of Veterinary Sciences

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Metabolic activity of boar spermatozoa, liquid stored for three days at 5 o C, was measured using bioluminescence for ATP content, fluorescent assay (JC fluorochrome) of mitochondrial activity and oxygen consumption. Sperm motility and plasma membrane integrity (PMI) were simultaneously analyzed. Apart from the statistically significant effect (P < 0.001) of semen storage time, the importance of the individual source of the ejaculate for the analyzed parameters of metabolic efficiency of spermatozoa was shown. This phenomenon was manifested in the interaction of the individual source of the ejaculate with spermatozoa motility, integrity of their membranes and metabolic activity with the passing time of semen preservation. Recorded results indicate that the individual factor may have a significant influence on the technological usefulness of boar spermatozoa for liquid storage. Quality analyses conducted on boar semen stored at 5 o C may be used for pre-selection of boars producing sperm with an enhanced tolerance to cold shock.

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Section: Discussionmentioning

confidence: 99%

Boar variability affects sperm metabolism activity in liquid stored semen at 5°C

Dziekońska¹,

Strzeżek²

2011

Polish Journal of Veterinary Sciences

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“…The ratio of ∑ DPA and DHA/ ∑ all other membrane fatty acids was significantly related to survival rate (plasma membrane integrity) of sperm for both Norwegian Landrace and Duroc boars. Thus Waterhouse et al [11] concluded that male-tomale differences in sperm survival rate after freezing and thawing may be partly related to the amount of long-chain PUFA in the sperm plasma membranes.…”

Section: Boarmentioning

confidence: 99%

“…Lipid and fatty acid composition of sperm cells differ not only for different animals [7,8] but also for different species [9][10][11][12], even for fertile and subfertile population of same species [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Mandal

Badyakar

Chakrabarty

2014

Advances in Andrology

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Lipid is an important constituent of cell membrane. Membrane lipid composition of spermatozoa has been correlated to different function. Many researchers have related membrane lipid with survival success after cryopreservation or cold shock. Sperm maturation and acrosome reactions are natural phenomenon, but cryopreservation or cold shock is not. Therefore, sperm cells are not programmed for such change and undergo stress. So the change in membrane lipid composition due to cold shock or cryopreservation may be looked upon as response of spermatozoa to a certain stressed condition. A significant body of research worked on the relationship between membrane lipid and fatty acid composition and ability of cell to tolerate adverse change in temperature. However, as the approach of different research groups was different, it is very difficult to compare the changes. Studies have been done with different species, ejaculated/seminal or epididymal sperm. Lipid analyses have been done with whole cell membrane isolated by different methods. Fatty acids estimated were from whole cell, plasma membrane, head membrane, or phospholipids. The cryopreservation condition, media composition, and diluents/cryoprotectants were also different. At this onset a comprehensive review is needed to cover changes of sperm membrane lipid composition of different species under different cryopreservation conditions.

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“…The sperm suspensions were loaded into 0.5 mL polyvinyl chloride medium-straws (Bio-Vet, Fleurance, France) and sealed with plasticine. All straws were placed horizontally on a rack and put into a chamber of the controlledrate freezer set to +5ºC [19][20][21][22]. The cooling/freezing rate was as follows: 3ºC/min from +5ºC to −5ºC and 1 min of holding time and thereafter 50ºC/min from −5ºC to −140ºC.…”

Section: Semen Freezing Processmentioning

confidence: 99%

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Kaeoket¹,

Chanapiwat²,

Tummaruk³

et al. 2010

Asian J Androl

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Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L −1 (group I, control), 5 mmol L −1 (group II), 10 mmol L −1 (group III) and 15 mmol L −1 (group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P < 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In conclusion, 5 or 10 mmol L −1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.

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Within and between breed differences in freezing tolerance and plasma membrane fatty acid composition of boar sperm

Cited by 161 publications

References 30 publications

Boar variability affects sperm metabolism activity in liquid stored semen at 5°C

Boar variability affects sperm metabolism activity in liquid stored semen at 5°C

Role of Membrane Lipid Fatty Acids in Sperm Cryopreservation

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

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