Within-Plant Accumulation of Potato Leafroll Virus by Aggregated Green Peach Aphid Feeding

Mowry, Thomas M.

doi:10.1094/phyto-85-859

Cited by 13 publications

(11 citation statements)

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“…It is indeed possible that PLRV movement along phloem sieve elements is an essentially passive process. It is, after all, known that the movement of PLRV, and many other viruses, is strongly dependent on metabolite fluxes and that movement of the virus in potato plants can be manipulated by adjusting sink strength (Mowry, 1995).…”

Section: Discussionmentioning

confidence: 99%

Short and long distance spread of potato leafroll luteovirus: effects of host genes and transgenes conferring resistance to virus accumulation in potato.

Derrick¹,

Barker²

1997

Journal of General Virology

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Section: Discussionmentioning

confidence: 99%

Short and long distance spread of potato leafroll luteovirus: effects of host genes and transgenes conferring resistance to virus accumulation in potato.

Derrick¹,

Barker²

1997

Journal of General Virology

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“…In arboviruses, which infect animals, the insect’s saliva may contain substances that assist the virus in transmission to its host, a phenomenon termed Saliva Activated Transmission (SAT). Such factors are just now being discovered in the saliva of insect vectors of plant viruses, though it has been reported that aggregates of viruliferous aphids on a healthy host can form higher concentration of the virus in their feeding area than the rest of the plant [15,73,74,75,76,77]. On the other hand, no insect vector that has the ability to resist virus ingestion and/or transmission exists, although examples of viruses naturally losing their transmission ability have been described [70,71,78,79,80,81].…”

Section: Proteins Influencing the Circulative Transmission Of Planmentioning

confidence: 99%

The Role of Bacterial Chaperones in the Circulative Transmission of Plant Viruses by Insect Vectors

Kliot

Ghanim

2013

Viruses

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Persistent circulative transmission of plant viruses involves complex interactions between the transmitted virus and its insect vector. Several studies have shown that insect vector proteins are involved in the passage and the transmission of the virus. Interestingly, proteins expressed by bacterial endosymbionts that reside in the insect vector, were also shown to influence the transmission of these viruses. Thus far, the transmission of two plant viruses that belong to different virus genera was shown to be facilitated by a bacterial chaperone protein called GroEL. This protein was shown to be implicated in the transmission of Potato leafroll virus (PLRV) by the green peach aphid Myzus persicae, and the transmission of Tomato yellow leaf curl virus (TYLCV) by the sweetpotato whitefly Bemisia tabaci. These tri-trophic levels of interactions and their possible evolutionary implications are reviewed.

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“…In most studies it is assumed that when the controls show 100% of infection, the viral transmission was successful for the transgenic lines tested in parallel. The amount of luteoviruses has been reported to vary among different leaves on the same plant (Pereira and Lister, 1989;Mowry, 1995). This effect was minimised in this study by taking leaf discs from three different levels of each BWYV inoculated plant.…”

Section: Discussionmentioning

confidence: 99%

Transformation of Nicotiana benthamiana with different BWYV (Beet western yellows virus) sequences to test for virus resistance

Valenzuela

Laucke

Schiemann

2005

Plant Cell Tiss Organ Cult

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Nicotiana benthamiana plants were transformed by means of Agrobacterium tumefaciens carrying various constructs to test for resistance for BWYV (Beet western yellows virus), the constructs used included either the viral replicase (ORF1/2), one of two smaller sequences involving the 5¢ and 3¢ ends (5¢3¢S and 5¢3¢AS) of the viral genome of BWYV. According to different criteria such as ELISA, PCR, growth under kanamycin selection at 200 mg l )1 and phenotype some lines were chosen as candidates to be tested for resistance against BWYV. Five lines of each construct were randomly selected. These lines were analysed by Northern blot for expression of the transgene of interest and/or the nptII gene. Greenhouse resistance tests were performed in 15 transgenic N. benthamiana lines (5 per construct) and in two controls. The transgenic plantlets were transferred to the greenhouse and 20 plants from each line were inoculated with BWYV using the natural vector Myzus persicae, while other 20 were kept as uninoculated control. At 4, 6 and 8 weeks post-infection (wpi) a BWYV ELISA of the inoculated plants was carried out and the height of each plant was measured, while the weight was determined at the end of the experiment. None of the transgenic lines tested showed resistance to the virus.Abbreviations: 5¢3¢S -5¢3¢ sense; 5¢3¢AS -5¢3¢ antisense; BWYV -beet western yellows virus; CP -coat protein; dpi -days post infection; Km -kanamycin; ORF -open reading frame; PEBV -pea early browning virus; PLRV -potato leafroll virus; PMMV -pepper mild mosaic virus; wpi -weeks' postinfection

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Within-Plant Accumulation of Potato Leafroll Virus by Aggregated Green Peach Aphid Feeding

Cited by 13 publications

References 0 publications

Short and long distance spread of potato leafroll luteovirus: effects of host genes and transgenes conferring resistance to virus accumulation in potato.

Short and long distance spread of potato leafroll luteovirus: effects of host genes and transgenes conferring resistance to virus accumulation in potato.

The Role of Bacterial Chaperones in the Circulative Transmission of Plant Viruses by Insect Vectors

Transformation of Nicotiana benthamiana with different BWYV (Beet western yellows virus) sequences to test for virus resistance

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