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Abstract

Cissus latifolia Lam. (Vitaceae) is a woody climber with leafopposed tendrils. It is a medicinal plant used in the traditional system of medicine for the treatment of various ailments. The present study focused on the identification and qualitative determination of phytoconstituent types and establishment of the HPTLC fingerprint profile of the hot and cold extracts of C. latifolia. Preliminary phytochemical screening was done to identify the class of compounds present. HPTLC analyses of eight different extracts were carried out with the most suitable mobile phase system using the Camag HPTLC instrument consisting of Linomat-V automated spotter having a 100 μl syringe connected to a nitrogen cylinder, twintrough developing chamber, scanner-III and viewing cabinet with dual wavelength UV lamps (Camag, Muttenz, Switzerland). Qualitative phytochemical screening revealed the presence of flavonoids, coumarins, tannins, alkaloids, steroids, terpenoids, saponins, quinines, anthraquinones and phenol in the stem of C. latifolia. The HPTLC profiling of eight different extracts showed the presence of alkaloids, flavonoids, phenols, saponins, and tannins with different R f values. The results of preliminary phytochemical screening and HPTLC fingerprint obtained from this study can be used as a reference for the standardization and quality control of Cissus latifolia stem. INTRODUCTION: According to Angiosperm Phylogeny Group IV classification, the family Vitaceae consists of two subfamilies Vitoideae (Eaton) and Leeoideae (Burmeister) 1 and Cissus is the largest genus in the family with about 350 species 2. Cissus L. is seen widely distributed in the tropical regions such as Africa (ca.135 species), Southern Asia (ca. 85 species), Australia (ca.12 species) and the Americas (77 species) 3. C. latifolia is a woody climber with leaf-opposed tendrils, which may be modified to form an inflorescence.